Use of the johnin PPD interferon-gamma assay in control of bovine paratuberculosis

Jungersen, Gregers; Mikkelsen, Heidi; Grell, S. N.

doi:10.1016/j.vetimm.2011.05.010

Cited by 43 publications

(36 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This has previously been noted by Jungersen et al in relation to bovine paratuberculosis (Jungersen et al, 2012). We are able to expand on this and report that although all exposed sheep have an IFNγ response, the strength of this early IFNγ response reflects future disease outcome.…”

Section: Discussionsupporting

confidence: 80%

“…IFNγ activates bystander macrophages and facilitates intracellular killing of MAP. While the IFNγ response is important in the cell-mediated control of intracellular pathogens like MAP it is not always a predictor of disease outcome (Jungersen et al, 2012). To counteract the host's immune response, MAP can also actively induce certain cytokines to suppress and evade immune cells; interleukin (IL)-10 and tumour growth factor (TGF)β are two such cytokines (Weiss and Souza, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Can early host responses to mycobacterial infection predict eventual disease outcomes?

Silva

Begg

Plain

et al. 2013

Preventive Veterinary Medicine

View full text Add to dashboard Cite

This is a pre-copyedited, author-produced PDF of an article published in [Prev Vet Med] AbstractDiagnostic tests used for Johne's disease in sheep either have poor sensitivity and specificity or only detect disease in later stages of infection. Predicting which of the infected sheep are likely to become infectious later in life is currently not feasible and continues to be a major hindrance in disease control. We conducted this longitudinal study to investigate if a suite of diagnostic tests conducted in Mycobacterium avium subspecies paratuberculosis (MAP) exposed lambs at 4 months post infection can accurately predict their clinical status at 12 months post infection. We tracked cellular and humoral responses and quantity of MAP shedding for up to 12 months post challenge in 20 controls and 37 exposed sheep. Infection was defined at necropsy by tissue culture and disease spectrum by lesion type. Data were analysed using univariable and multivariable logistic regression models and a subset of variables from the earliest period post inoculation (4 months) was selected for predicting disease outcomes later on (12 months). Sensitivity and specificity of tests and their combinations in series and parallel were determined. Early elevation in faecal MAP DNA quantity and a lower interferon gamma (IFNγ) response were significantly associated with sheep becoming infectious as well as progressing to severe disease. Conversely, early low faecal MAP DNA and higher interleukin-10 responses were significantly associated with an exposed animal developing protective immunity. Combination of early elevated faecal MAP DNA or lower IFNγ response had the highest sensitivity (75%) and specificity (81%) for identifying sheep that would become infectious. Collectively, these results highlight the potential for combined test interpretation to aid in the early prediction of sheep susceptibility to MAP infection.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Can early host responses to mycobacterial infection predict eventual disease outcomes?

Silva

Begg

Plain

et al. 2013

Preventive Veterinary Medicine

View full text Add to dashboard Cite

show abstract

“…As expected, the sensitivity of serological PTB diagnosis strongly varied between both profiles (Nielsen & Toft, 2008;Jungersen et al, 2012).…”

Section: Discussionsupporting

confidence: 75%

“…From a diagnostic point of view, difficulties for invivo detection of latent form cases compared to patent form cases have been repeatedly reported, and are the main cause of failure of some radical testing and culling eradication programs (Jungersen et al, 2012). As expected, the sensitivity of serological PTB diagnosis strongly varied between both profiles (Nielsen & Toft, 2008;Jungersen et al, 2012).…”

Section: Discussionmentioning

confidence: 83%

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

Vázquez¹,

Garrido²,

Molina³

et al. 2014

Span. j. agric. res.

View full text Add to dashboard Cite

Paratuberculosis is a chronic mycobacterial infection causing granulomatous enteritis in ruminants, whose pathogenesis and epidemiology poses numerous challenges, including latency and reactivation. The most recent and complete classification of paratuberculosis immunopathological types in cattle recognized five categories. In this study, 1031 slaughtered Friesian cattle were submitted to serological, microbiological and pathological examinations with the aim of maximizing the rate of case detection. In most cases, infected animals had minimal lesions and almost no other proof of infection (38.9%), while the more characteristic types with the whole constellation of microbiological and immunological evidences accounted for a lower proportion (7.7%). As these findings in cattle suggest similarities with the epidemiology of tuberculosis in humans, we propose to re-group the original immunopathological types into two broader paratuberculosis epidemio-pathogenic forms or states: latent and patent. The former term would define infections with focal lesions and might constitute an apparent resilience status representing a difficult to detect reservoir of infection whose role could become critical if later immune-compromising factors lead to re-activation. The latter would group those cases with multifocal and diffuse inflammatory lesions with higher mycobacterial load and viability suggestive of a more immediate epidemiological risk. Interestingly, the relative frequency of presentation of each profile varied with age. The proportion of latent forms remained relatively constant between 33.8% and 54.3% through adulthood from 3 years of age, while patent forms were more frequent during the first years of age and tended to decrease among the oldest individuals.

show abstract

“…The classical response pattern to mycobacteria infection involves firstly a T helper (Th) 1 response which then switches to a Th2 response (Stabel, 2000(Stabel, , 2006. The IFN-γ release assay (IGRA) takes advantage of this by assessing the cell mediated immune (CMI) response to purified protein derivatives (PPDs) (Jungersen et al, 2011) or recombinant antigens selected for their specificity (Gurung et al, 2014;Hughes et al, 2013;Mikkelsen et al, 2011a). This method requires antigens capable of eliciting a sensitive and specific response, and which are currently under investigation (Mikkelsen et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

Interferon gamma response to Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigen L5P in cattle

Holbert

Branger

Souriau

et al. 2015

Research in Veterinary Science

View full text Add to dashboard Cite

Use of the johnin PPD interferon-gamma assay in control of bovine paratuberculosis

Cited by 43 publications

References 16 publications

Can early host responses to mycobacterial infection predict eventual disease outcomes?

Can early host responses to mycobacterial infection predict eventual disease outcomes?

Latent infections are the most frequent form of paratuberculosis in slaughtered Friesian cattle

Interferon gamma response to Mycobacterium avium subsp. paratuberculosis specific lipopentapeptide antigen L5P in cattle

Contact Info

Product

Resources

About