Use of the polymerase chain reaction to study the relationship between human papillomavirus infections and cervical cancer

Melchers, Willem J. G.; Claas, H. C. J.; Quint, W. G. V.

doi:10.1007/bf01972496

Cited by 17 publications

(6 citation statements)

References 67 publications

(62 reference statements)

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“…We found a high incidence of HPV in women with normal cervical smears (38%) or with atypical squamous cells of undetermined significance in their cervical smears (51%) compared to other data obtained in the Dutch population or European women. 16 This reflects both the higher sensitivity of the system compared to other general HPV detection systems as described previously, as well as (and probably mainly) patient selection bias. 9,32 Women were incorporated into the study who were referred to the gynecologist after at least two successive cervical smears containing cells consistent with atypical squamous cells of undetermined significance.…”

Section: Discussionmentioning

confidence: 77%

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Short Fragment Polymerase Chain Reaction Reverse Hybridization Line Probe Assay to Detect and Genotype a Broad Spectrum of Human Papillomavirus Types

Melchers

Bakkers

Wang

et al. 1999

The American Journal of Pathology

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The purpose of this study was to detect and genotype 16 different human papilloma virus (HPV) types simultaneously using a short fragment polymerase chain reaction (SPF) hybridization line probe assay (LiPA). 152 women who were referred to the gynecologist because of abnormal cervical smear underwent colposcopic examination and repeat cervical smear. In addition, the cervical scrapes were analyzed for the presence of HPV by a novel general HPV polymerase chain reaction assay followed by a single reaction genotyping assay allowing for a simultaneous detection and identification of 16 different HPV types. HPV DNA was detected in 38% of normal follow-up cervical scrapes, 51% of scrapes with atypical squamous cells of undetermined significance, 78% of scrapes with mild dysplasia (low grade squamous intraepithelial lesions), 86% of scrapes with moderate dysplasia (high grade squamous intraepithelial lesions), and in 88% of scrapes with severe dysplasia and carcinoma in situ. One case of invasive squamous cell carcinoma was positive for HPV 16. Overall, a single HPV type was detected in 56% of HPV positive scrapes, with HPV 16 being the most common and accounting for 45% of all single infections. Forty-four percent of the positive scrapes contained multiple HPV types, of which double infections prevailed. Follow-up results proved the reproducibility and reliability of SPF HPV LiPA. In conclusion, we have used and evaluated the SPF-HPV-LiPA system for the detection and genotyping of HPV infections. The combined detection-typing method proved to be sensitive, specific, simple, and fast, making mass screening of cervical scrapes accessible for routine practice and facilitating individual patient management.

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Section: Discussionmentioning

confidence: 77%

“…The distribution of high risk HPV types (16,18,31,33,35,45, 51, 52, 56, and 58) and low risk HPV types (6, 11, 40, 42, 43, and 44) in the scrapes is shown in Figure 2. Prevalence of high risk HPV types was increasing with the severity of cervical smear abnormality.…”

Section: Low Risk Versus High Risk Hpv Typesmentioning

confidence: 99%

Short Fragment Polymerase Chain Reaction Reverse Hybridization Line Probe Assay to Detect and Genotype a Broad Spectrum of Human Papillomavirus Types

Melchers

Bakkers

Wang

et al. 1999

The American Journal of Pathology

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“…PCR may be the method of choice for screening because of high sensitivity and the ease of obtaining adequate specimens. [27][28][29] For special purposes, when single infected cells need to be identified and/or morphological relationships clarified, ISH is the method of choice. In our study, this was exemplified by the finding of additional HPV positive cases with few infected cells that were not detected by PCR.…”

Section: Discussionmentioning

confidence: 99%

HPV detection in cytological cases with condylomatous or dysplastic changes: A study with PCR and in situ hybridization on cytological material

Skyldberg

Hagmar

Johansson

et al. 1995

Diagnostic Cytopathology

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Cytobrush samples of 80 patients, who previously had a cytological or histopathological diagnosis of condyloma and/or dysplasia were investigated for human papillomavirus infection (HPV) by polymerase chain reaction (PCR) and in situ DNA hybridization technique (ISH). The results were compared with concomitantly obtained cytological Pap-stained smears or, in some cases, histological sections. The time between the diagnosis of the original and the concomitant cytology/histopathology was less than 1 yr. Six additional patients had similar morphological diagnoses 2-4 yr before. Five more cases were included on clinical diagnosis of HPV. Compared with the original morphological diagnoses, 70% of the cases were positive by PCR and/or ISH. The concomitant morphology was not diagnostic of HPV in 44 out of 80 cases (55%), showing a relatively high percentage of cases morphologically normalized in the interval since the first specimen was taken. After detection with PCR, 30 cases (37.5%) were negative for HPV. Only one of the patients with a previous disease 2-4 yr before was HPV positive by PCR and two out of five patients with a clinical diagnosis of HPV. ISH could be performed on 67/80 cases, 43 of which were positive for HPV. There was a good agreement between the results of ISH and PCR, but there were six cases positive by ISH and negative by PCR. In these cases, few infected cells may have escaped detection by PCR. Both methods seem to be able to detect silent HPV infections and comparison with concomitant cytology/histopathology shows that morphology alone is insufficient for HPV detection in these cases.

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“…In lymph nodes, the rate of viral detection drops to 31‐42% of samples. The viral serotype detected in the tumor has previously been shown to be prognostic 235. HPV‐16 and HPV‐18 are associated with more malignant tumors than HPV‐6 and HPV‐8.…”

Section: Clinical Studiesmentioning

confidence: 99%

Utilization of polymerase chain reaction technology in the detection of solid tumors

Raj

Moreno

Gomella

1998

Cancer

160

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PCR can detect tumor marker-expressing cells that are otherwise undetectable by other means in patients with localized or metastatic cancer. Reports from various study groups have lacked uniformity in their protocols, and this has prevented adequate comparison. The clinical utility of this assay as a tool for the prognosis and management of cancer patients remains and area of active investigation. PCR is a powerful tool in the study of the biology of cancer metastasis and will likely serve as a useful adjunct to clinical decision-making in the future.

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Use of the polymerase chain reaction to study the relationship between human papillomavirus infections and cervical cancer

Cited by 17 publications

References 67 publications

Short Fragment Polymerase Chain Reaction Reverse Hybridization Line Probe Assay to Detect and Genotype a Broad Spectrum of Human Papillomavirus Types

Short Fragment Polymerase Chain Reaction Reverse Hybridization Line Probe Assay to Detect and Genotype a Broad Spectrum of Human Papillomavirus Types

HPV detection in cytological cases with condylomatous or dysplastic changes: A study with PCR and in situ hybridization on cytological material

Utilization of polymerase chain reaction technology in the detection of solid tumors

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