Use of the potential probiotic strain Lactobacillus salivarius SMXD51 to control Campylobacter jejuni in broilers

Saint-Cyr, Manuel Jimmy; Haddad, Nabila; Taminiau, Bernard; Poëzévara, Typhaine; Quesne, Segolénè; Amelot, Michel; Daube, Georges; Chemaly, Marianne; Dousset, Xavier; Guyard‐Nicodème, Muriel

doi:10.1016/j.ijfoodmicro.2016.07.003

Cited by 88 publications

(86 citation statements)

References 65 publications

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“…None of the three treatments (T3, T4, and T7) used from day 1 was able to prevent Campylobacter colonization detected at day 42. Similar results were observed in previous works testing several feed additives in experimental facilities with artificial Campylobacter contamination on fast-growing broilers (Gracia et al, 2016; Guyard-Nicodeme et al, 2016; Saint-Cyr et al, 2016b). Moreover, no treatment using single product (T2, T3, T4, T5, and T7) led to a significant reduction of Campylobacter in caeca, compared to the control group at the end of the rearing period.…”

Section: Discussionsupporting

confidence: 91%

“…Feed additives with non-antibiotic products such as probiotic bacteria, prebiotics, plant extracts or organic acids against Campylobacter colonization are still extensively studied. They give some promising results in experimental trials leading to at least 2 log 10 CFU/g reduction or more in Campylobacter colonization for some of them (Skanseng et al, 2010; Ghareeb et al, 2012; Guyard-Nicodeme et al, 2016; Saint-Cyr et al, 2016b). In these studies testing feed additives, trials were generally performed using conventional production conditions: indoor rearing, broiler breeds, whole rearing (Hilmarsson et al, 2006; Thibodeau et al, 2015; Gracia et al, 2016; Guyard-Nicodeme et al, 2016), or shorter periods (Solis de Los Santos et al, 2008; Skanseng et al, 2010; Ghareeb et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Feed Additives on Productivity and Campylobacter spp. Loads in Broilers Reared under Free Range Conditions

et al. 2017

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The poultry reservoir, especially broiler meat, is generally recognized as one of the most-important sources for human Campylobacteriosis. The measures to control Campylobacter targeted essentially the primary production level. The aim of this work was to evaluate the effectiveness of different treatments against natural Campylobacter colonization in a French experimental farm of free-range broilers during the whole rearing period. Five commercial products and a combination of two of them were tested and all the products were added to feed or to water at the dose recommended by the suppliers. Campylobacter loads in caeca and on carcasses of broilers at the slaughter were determined by culture methods. Natural contamination of the flock occurred at the end of the indoor rearing period between day 35 and day 42. At day 42, the multispecies probiotic added to the feed reduced the contamination of 0.55 log10 CFU/g (p = 0.02) but was not significant (p > 0.05) at the end of rearing at day 78. However, another treatment, a combination of a cation exchange clay-based product in feed and an organic acid mixture (formic acid, sodium formate, lactic acid, propionic acid) in water, led to a slight but significant reduction of 0.82 ± 0.25 log10 CFU/g (p = 0.02) compared to the control group at day 78. Testing this combination in field conditions in several flocks is needed to determine if it is biologically relevant and if it could be a valuable measure to reduce Campylobacter in broiler flocks.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effect of Feed Additives on Productivity and Campylobacter spp. Loads in Broilers Reared under Free Range Conditions

et al. 2017

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“…In order to prepare for the future ban on the use of antibiotics in North America, there is a growing need for new nonantibiotic alternatives to improve bird performance and simultaneously prevent the spread of zoonotic pathogens of human health importance, such as C. jejuni. Probiotics are already widely used as an alternative to antibiotics (9,29,30) in order to improve feed conversion. Several studies, with various results, also described the use of food additives, including oligofructose or organic acids (31,32), in addition to single or mixed cultures of bacteria such as Lactobacillus, Enterococcus, and Bifidobacterium species to reduce C. jejuni levels in chickens (9).…”

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confidence: 99%

Coadministration of the Campylobacter jejuni N-Glycan-Based Vaccine with Probiotics Improves Vaccine Performance in Broiler Chickens

Nothaft

Pérez-Muñoz

Gouveia

et al. 2017

Appl Environ Microbiol

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Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chickens, but only a fraction of birds respond to vaccination. Optimization of the vaccine for commercial broiler chickens has great potential to prevent the entry of the pathogen into the food chain. Here, we tested the same vaccination approach in broiler chickens and observed similar efficacies in pathogen load reduction, stimulation of the host IgY response, the lack of C. jejuni resistance development, uniformity in microbial gut composition, and the bimodal response to treatment. Gut microbiota analysis of leghorn and broiler vaccine responders identified one member of Clostridiales cluster XIVa, Anaerosporobacter mobilis, that was significantly more abundant in responder birds. In broiler chickens, coadministration of the live vaccine with A. mobilis or Lactobacillus reuteri, a commonly used probiotic, resulted in increased vaccine efficacy, antibody responses, and weight gain. To investigate whether the respondernonresponder effect was due to the selection of a C. jejuni "supercolonizer mutant" with altered phase-variable genes, we analyzed all poly(G)-containing loci of the input strain compared to nonresponder colony isolates and found no evidence of phase state selection. However, untargeted nuclear magnetic resonance (NMR)-based metabolomics identified a potential biomarker negatively correlated with C. jejuni colonization levels that is possibly linked to increased microbial diversity in this subgroup. The comprehensive methods used to examine the bimodality of the vaccine response provide several opportunities to improve the C. jejuni vaccine and the efficacy of any vaccination strategy.IMPORTANCE Campylobacter jejuni is a common cause of human diarrheal disease worldwide and is listed by the World Health Organization as a high-priority pathogen. C. jejuni infection typically occurs through the ingestion of contaminated chicken meat, so many efforts are targeted at reducing C. jejuni levels at the source. We previously developed a vaccine that reduces C. jejuni levels in egg-laying chickens. In this study, we improved vaccine performance in meat birds by supplementing the vaccine with probiotics. In addition, we demonstrated that C. jejuni colonization levels in chickens are negatively correlated with the abundance of clostridia, another group of common gut microbes. We describe new methods for vaccine op-

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“…The few studies that evaluated the impact of C. jejuni colonization on the chicken caecal microbiota all showed that the microbiota structure is somewhat affected, but different conclusions were reached when comparing microbiota compositional changes (Johansen et al, 2006; Sofka et al, 2015; Saint-Cyr et al, 2016). This might be due to the use of different DNA extraction methods, different 16S rRNA gene regions being sequenced, and different bioinformatics pipelines used to process raw sequences (de la Cuesta-Zuluaga and Escobar, 2016).…”

Section: Discussionmentioning

confidence: 99%

Lack of Evidence That Selenium-Yeast Improves Chicken Health and Modulates the Caecal Microbiota in the Context of Colonization by Campylobacter jejuni

et al. 2017

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Faced with ever-increasing demand, the industrial production of food animals is under pressure to increase its production. In order to keep productivity, quality, and safety standards up while reducing the use of antibiotics, farmers are seeking new feed additives. In chicken production, one of these additives is selenium. This element is expected to confer some advantages in terms of animal health and productivity, but its impact on chicken intestinal microbiota as well as on the carriage of foodborne pathogens is unknown. In this study, chickens raised in a level 2 animal facility were fed or not 0.3 ppm of in-feed selenium-yeast until 35 days of age and were inoculated or not with the foodborne pathogen Campylobacter jejuni at the age of 14 days. At the end of the study, body weight, seric IgY, intestinal IgA, seric gluthatione peroxydase activity, the caecal microbiota (analyzed by MiSeq 16S rRNA gene sequencing), and C. jejuni caecal levels were analyzed. The experiment was completely replicated twice, with two independent batches of chickens. This study revealed that, for healthy chickens raised in very good hygienic conditions, selenium-yeast does not influence the bird’s body weight and lowers their seric gluthatione peroxidase activity as well as their intestinal IgA concentrations. Furthermore, selenium-yeast did not modify the caecal microbiota or the colonization of C. jejuni. The results also showed that C. jejuni colonization does not impact any of the measured chicken health parameters and only slightly impacts the caecal microbiota. This study also clearly illustrated the need for true biological replication (independent animal trials) when assessing the microbiota shifts associated with treatments as the chickens microbiotas clearly clustered according to study replicate.

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Use of the potential probiotic strain Lactobacillus salivarius SMXD51 to control Campylobacter jejuni in broilers

Cited by 88 publications

References 65 publications

Effect of Feed Additives on Productivity and Campylobacter spp. Loads in Broilers Reared under Free Range Conditions

Effect of Feed Additives on Productivity and Campylobacter spp. Loads in Broilers Reared under Free Range Conditions

Coadministration of the Campylobacter jejuni N-Glycan-Based Vaccine with Probiotics Improves Vaccine Performance in Broiler Chickens

Lack of Evidence That Selenium-Yeast Improves Chicken Health and Modulates the Caecal Microbiota in the Context of Colonization by Campylobacter jejuni

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