Use of the receptor tyrosine kinase-like orphan receptor 1 (ROR1) as a diagnostic tool in chronic lymphocytic leukemia (CLL)

Uhrmacher, Sabrina; Schmidt, Christina; Erdfelder, Felix; Poll-Wolbeck, Simon Jonas; Gehrke, Iris; Hallek, Michael; Kreuzer, Karl‐Anton

doi:10.1016/j.leukres.2011.04.006

Cited by 41 publications

(50 citation statements)

References 17 publications

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“…The prerequisite of investigating the behaviors of patient cancer cells is recognizing the cancer cells in the biopsy samples prepared from the patient. In recent years, studies have shown that ROR1 is an effective biomarker which is exclusively expressed on some leukemias and B-cell lymphoma cells (e.g., mantle cell lymphoma cell, follicular lymphoma cell, and marginal zone lymphoma cell) but not on almost all normal cells (Uhrmacher et al, 2011;Baskar et al, 2012;Zhang et al, 2012). Here we collected the bone marrow from a mantle cell lymphoma patient with bone marrow invasion.…”

Section: Discussionmentioning

confidence: 99%

“…After washing the slides with PBS for three times, the slides were put on the stage of an inverted fluorescence microscope (Ti, Nikon, Japan) for experiments. ROR1 is a cellular surface marker which is expressed only on some types of cancer cells (such as chronic lymphocytic leukemia cell, mantle cell lymphoma cell, follicular lymphoma cell, and marginal zone lymphoma cell) but not expressed on normal cells (Uhrmacher et al, 2011;Baskar et al, 2012). Hence, for lymphoma patients whose bone marrow had been invaded by cancer cells, we can discern the cancer cells in the bone marrow via ROR1 fluorescence labeling.…”

Section: Patient Biopsy Cell Sample Preparationmentioning

confidence: 99%

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Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

Xiao

Liu

et al. 2016

Journal of Immunological Methods

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Section: Discussionmentioning

confidence: 99%

Section: Patient Biopsy Cell Sample Preparationmentioning

confidence: 99%

Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

Xiao

Liu

et al. 2016

Journal of Immunological Methods

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“…To investigate the CD20-rituximab interactions directly on cancer cells from lymphoma patients, the cancer cells from the clinical biopsy samples should first be identified because in biopsy samples (such as bone marrow), cancer cells and normal cells are mixed together. Receptor tyrosine-like orphan receptor 1 (ROR1) is a specific cell surface marker that is highly expressed on B lymphoma cancer cells, but not on normal cells [94,95] . Therefore, we used ROR1 to identify the cancer cells in the bone marrow samples prepared from B-cell lymphoma patients.…”

Section: Wwwchinapharcom LI M Et Almentioning

confidence: 99%

Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy

Liu

et al. 2015

Acta Pharmacol Sin

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Knowledge of the nanoscale changes that take place in individual cells in response to a drug is useful for understanding the drug action. However, due to the lack of adequate techniques, such knowledge was scarce until the advent of atomic force microscopy (AFM), which is a multifunctional tool for investigating cellular behavior with nanometer resolution under near-physiological conditions. In the past decade, researchers have applied AFM to monitor the morphological and mechanical dynamics of individual cells following drug stimulation, yielding considerable novel insight into how the drug molecules affect an individual cell at the nanoscale. In this article we summarize the representative applications of AFM in characterization of drug actions on cell membrane, including topographic imaging, elasticity measurements, molecular interaction quantification, native membrane protein imaging and manipulation, etc. The challenges that are hampering the further development of AFM for studies of cellular activities are aslo discussed.

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“…We are interested in the behaviors of tumor B cells, because their behaviors are directly related to the treatment outcomes. Recent studies have shown that the receptor tyrosine kinase-like orphan receptor 1 (ROR1) is exclusively expressed on B cell leukemia and lymphomas but not on normal B cells and other healthy tissues [41][42][43]. Hence, ROR1 is an adequate marker for identifying tumor B cells from lymphoma patients.…”

Section: Rituximab Actions On Patient Tumor Cellsmentioning

confidence: 99%

Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale

Liu

et al. 2015

BioNanoSci.

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Rituximab, a monoclonal antibody against the CD20 molecule expressed on B cells, has revolutionized the treatment of B cell lymphomas. The use of rituximab significantly improves the long-term survival rates of lymphoma patients. However, there are still many patients who develop resistance to rituximab. Hence, the urgent need is enhancing the potency of anti-CD20 antibodies beyond that achieved with rituximab to provide effective therapies for more patients. Traditional studies about the killing mechanisms of rituximab are commonly based on optical microscopy, which cannot reveal the detailed situations due to the limit of 200-nm resolution. Quantitatively investigating the actions of rituximab at the nanoscale is still scarce. The advent of atomic force microscopy (AFM) provides a powerful and versatile platform for in situ investigating the nanoscale behaviors of individual live cells. The applications of AFM in life sciences in the past decade have yielded a lot of novel insights into cell biology and related diseases. In this article, the typical applications (e.g., ultra-microstructures, mechanical properties, and molecular recognition) of AFM in investigating the three killing mechanisms of rituximab at the nanoscale are summarized; the challenges facing AFM single-cell assay and its future directions are also discussed.

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Use of the receptor tyrosine kinase-like orphan receptor 1 (ROR1) as a diagnostic tool in chronic lymphocytic leukemia (CLL)

Cited by 41 publications

References 17 publications

Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy

Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy

Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale

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