Use of the Signature Fatty Acid 16:1<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi mathvariant="bold">ω</mml:mi></mml:mrow></mml:math>5 as a Tool to Determine the Distribution of Arbuscular Mycorrhizal Fungi in Soil

Ngosong, Christopher; Gabriel, Elke; Rueß, Liliane

doi:10.1155/2012/236807

Cited by 89 publications

(53 citation statements)

References 64 publications

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“…The sum of 18:2ω6,9, 18:3ω6, 18:3ω3 was used to represent total fungal PLFAs (Frostegård and Bååth 1996;Ruess and Chamberlain 2010). The NLFA 16:1ω5c was used as marker for arbuscular mycorrhizal fungi (AMF; nmol g −1 dry litter/soil material; Lekberg et al 2012;Ngosong et al 2012).…”

Section: Microorganismsmentioning

confidence: 99%

Impact of tropical lowland rainforest conversion into rubber and oil palm plantations on soil microbial communities

et al. 2015

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Focusing on Sumatra, a hotspot of tropical lowland rainforest transformation, we investigated effects of the conversion of rainforests into rubber agroforests (Bjungle rubber^), intensive rubber, and oil palm plantations on the communities of litter and soil microorganisms and identified factors responsible for these changes. Litter basal respiration, microbial biomass, total bacterial phospholipid fatty acids (PLFAs), and fungal PLFAs did not vary significantly with rainforest conversion. In litter of converted ecosystems, the concentration of certain PLFAs including the Gram-negative bacteria marker PLFA cy17:0 and the Gram-positive bacteria marker PLFA i17:0 was reduced as compared to rainforest, whereas the concentration of the arbuscular mycorrhizal fungi (AMF) marker neutral lipid fatty acid (NLFA) 16:1ω5c increased. As indicated by redundancy analysis, litter pH and carbon concentration explained most of the variation in litter microbial community composition. In soil, microbial biomass did not vary significantly with rainforest conversion, whereas basal respiration declined. Total PLFAs and especially that of Gram-negative bacteria decreased, whereas PLFA i17:0 increased with rainforest conversion. The concentration of fungal PLFAs increased with rainforest conversion, whereas NLFA 16:1ω5c did not change significantly. Redundancy analysis indicated that soil pH explained most of the variation in soil microbial community composition. Overall, the data suggest that conversion of rainforests into production systems results in more pronounced changes in microbial community composition in soil as compared to litter. In particular, the response of fungi and bacteria was more pronounced in soil, while the response of AMF was more pronounced in litter. Notably, only certain bacterial markers but not those of saprotrophic fungi and AMF were detrimentally affected by rainforest conversion.

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Section: Microorganismsmentioning

confidence: 99%

Impact of tropical lowland rainforest conversion into rubber and oil palm plantations on soil microbial communities

et al. 2015

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“…Nevertheless, based on the large number of studies in which typical higherplant-derived patterns of lipids are reported and used in soils (Table 1), including indicative ACL and CPI values, microbial input of longer chain length straight-chain lipids generally does not seem to be a major factor compared to directplant-derived input in the topsoil Bai et al, 2009). In contrast, for steroids and triterpenoids such as camposterol, stigmasterol, and lupeol, microbial input in soils can be considerable (Naafs et al, 2004a). As another example, arbuscular mycorrhizal fungi-derived β-sitosterol is by far the most abundant sterol identified in soils (Grandmougin-Ferjani et al, 1999).…”

Section: Microbial Inputmentioning

confidence: 99%

“…For instance, the oxidation of free fatty acids could be a source of ω-hydroxy fatty acids, whereas the microbial β-oxidation of unsaturated fatty acids and/or mid-chain hydroxy fatty acids may be a source of α-and ω-alkanediotic acids, thus clouding the cutin or suberin signal (Naafs et al, 2004b).…”

Section: Microbial Inputmentioning

confidence: 99%

“…Generally, sterols, diterpenes, and pentacyclic, triterpenes are reported to be turned over rapidly compared to straight-chain lipids in grassland and forest soils, hindering their application as molecular proxies for their sources (Bull et al, 2000;Naafs et al, 2004b;Jansen et al, 2007). However, observed the exact opposite trend, indicating a strong environmental control on the relative transformation rate of different classes of components.…”

Section: Isoprenoidsmentioning

confidence: 99%

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Opportunities and limitations related to the application of plant-derived lipid molecular proxies in soil science

Jansen

Wiesenberg

2017

SOIL

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Abstract. The application of lipids in soils as molecular proxies, also often referred to as biomarkers, has dramatically increased in the last decades. Applications range from inferring changes in past vegetation composition, climate, and/or human presence to unraveling the input and turnover of soil organic matter (SOM). The molecules used are extractable and non-extractable lipids, including ester-bound lipids. In addition, the carbon or hydrogen isotopic composition of such molecules is used. While holding great promise, the application of soil lipids as molecular proxies comes with several constraining factors, the most important of which are (i) variability in the molecular composition of plant-derived organic matter both internally and between individual plants, (ii) variability in (the relative contribution of) input pathways into the soil, and (iii) the transformation and/or (selective) degradation of (some of) the molecules once present in the soil. Unfortunately, the information about such constraining factors and their impact on the applicability of molecular proxies is fragmented and scattered. The purpose of this study is to provide a critical review of the current state of knowledge with respect to the applicability of molecular proxies in soil science, specifically focusing on the factors constraining such applicability. Variability in genetic, ontogenetic, and environmental factors influences plant n-alkane patterns in such a way that no unique compounds or specific molecular proxies pointing to, for example, plant community differences or environmental influences, exist. Other components, such as n-alcohols, n-fatty acids, and cutin-and suberin-derived monomers, have received far less attention in this respect. Furthermore, there is a high diversity of input pathways offering both opportunities and limitations for the use of molecular proxies at the same time. New modeling approaches might offer a possibility to unravel such mixed input signals. Finally, the transformation and turnover of SOM offer opportunities when tracing such processes is the purpose of applying a molecular proxy while imposing limitations when they obliterate the molecular proxy signals linked to other phenomena. For n-alkanes several modeling approaches have recently been developed to compensate for (selective) degradation. Still, such techniques are in their infancy and information about their applicability to classes of components other than n-alkanes is lacking. All constraining factors considered can have a significant influence on the applicability of molecular proxies in soil science. The degree of influence strongly depends on the type of molecular proxy and the environmental context in which it is applied. However, the potential impact of the constraining factors should always explicitly be addressed whenever molecular proxies are applied in a soil scientific context. More importantly, there is still a serious lack of available information, in particular for compound classes other than the n-alkanes. Therefore, we...

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“…The PLFA 16:1u5 has been proposed as a biomarker for AMF. However, it was not used here as it is also found in considerable quantities in Gram negative bacteria (Ngosong et al, 2012;Ruess and Chamberlain, 2010;Sharma and Buyer, 2015). Individual PLFA's were analysed for d 13 C using GC -combustionisotope ratio mass spectrometry (GC-C-IRMS) (Isoprime Ltd).…”

Section: -Plfa Analysesmentioning

confidence: 99%

Functional differences in the microbial processing of recent assimilates under two contrasting perennial bioenergy plantations

Elias

Rowe

Pereira

et al. 2017

Soil Biology and Biochemistry

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a b s t r a c tLand use change driven alteration of microbial communities can have implications on belowground C cycling and storage, although our understanding of the interactions between plant C inputs and soil microbes is limited. Using phospholipid fatty acids (PLFA's) we profiled the microbial communities under two contrasting UK perennial bioenergy crops, Short Rotation Coppice (SRC) willow and Miscanthus Giganteus (miscanthus), and used Functional differences in microbial communities were highlighted by contrasting processing of labelled C. SRC willow allocated 44% of total 13 C detected into fungal PLFA relative to 9% under miscanthus and 380% more 13 C was returned to the atmosphere in soil respiration from SRC willow soil compared to miscanthus. Our findings elucidate the roles that bacteria and fungi play in the turnover of recent plant derived C under these two perennial bioenergy crops, and provide important evidence on the impacts of land use change to bioenergy on microbial community composition. Crown

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Use of the Signature Fatty Acid 16:1ω5 as a Tool to Determine the Distribution of Arbuscular Mycorrhizal Fungi in Soil

Cited by 89 publications

References 64 publications

Impact of tropical lowland rainforest conversion into rubber and oil palm plantations on soil microbial communities

Impact of tropical lowland rainforest conversion into rubber and oil palm plantations on soil microbial communities

Opportunities and limitations related to the application of plant-derived lipid molecular proxies in soil science

Functional differences in the microbial processing of recent assimilates under two contrasting perennial bioenergy plantations

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