Use of the viability reagent PrestoBlue in comparison with alamarBlue and MTT to assess the viability of human corneal epithelial cells

Xu, Mengyue; McCanna, David J.; Sivak, J. G.

doi:10.1016/j.vascn.2014.11.003

Cited by 153 publications

(130 citation statements)

References 21 publications

Supporting

Mentioning

128

Contrasting

Order By: Relevance

“…This effect is typical for many cytotoxic drugs and anticancer agents and can be interpreted as a rescue mechanism of the exposed cells [36]. In MTT assay, a minimum of 1000 cells per well is mandatory in comparison to PrestoBlue, which, according to Boncler et al [37] and Xu et al [38] requires only 12 cells per well. Therefore, PrestoBlue is a more sensitive assay than MTT.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Odorous Compounds from Poultry Manure

Nowak

Matusiak

Borowski

et al. 2016

IJERPH

View full text Add to dashboard Cite

Long-term exposure and inhalation of odorous compounds from poultry manure can be harmful to farm workers and the surrounding residents as well as animals. The aim of the present study was to determine the cytotoxicity and IC50 values of common odorous compounds such as ammonium, dimethylamine, trimethylamine, butyric acid, phenol, and indole in the chick liver hepatocellular carcinoma cell line LMH (Leghorn Male Hepatoma), in vitro, using MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and PrestoBlue cytotoxicity assays. The cells were microscopically examined for any morphological changes post treatment. Dimethylamine exhibited the strongest cytotoxic effect on LMH cells with an IC50 value of 0.06% and 0.04% after an exposure of 24 h and 48 h, respectively. Both ammonium and trimethylamine had comparable cytotoxicity and their IC50 values were 0.08% and 0.04% after 24 h and 48 h, respectively. Of note, indole had the lowest cytotoxicity as the majority of cells were viable even after 72 h exposure. Thus, the IC50 for indole was not calculated. Results achieved from both MTT and PrestoBlue assays were comparable. Moreover, the morphological changes induced by the tested odours in LMH cells resulted in monolayer destruction, cytoplasm vacuolisation, chromatin condensation, and changes in nucleus and cell shape. Our study showed harmful effects of odorous compounds in chick tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Odorous Compounds from Poultry Manure

Nowak

Matusiak

Borowski

et al. 2016

IJERPH

View full text Add to dashboard Cite

show abstract

“…Despite this complexity, numerous short-term colorimetric assays are being increasingly used to assess radiosensitivity and chemosensitivity [30,31,32,33,34,35]. Such assays are often performed in a multi-well plate format and the results (absorbance or fluorescence) are recorded in a plate reader.…”

Section: Introductionmentioning

confidence: 99%

Multinucleated Giant Cancer Cells Produced in Response to Ionizing Radiation Retain Viability and Replicate Their Genome

Mirzayans

Andrais

Scott

et al. 2017

IJMS

View full text Add to dashboard Cite

Loss of wild-type p53 function is widely accepted to be permissive for the development of multinucleated giant cells. However, whether therapy-induced multinucleation is associated with cancer cell death or survival remains controversial. Herein, we demonstrate that exposure of p53-deficient or p21WAF1 (p21)-deficient solid tumor-derived cell lines to ionizing radiation (between 2 and 8 Gy) results in the development of multinucleated giant cells that remain adherent to the culture dish for long times post-irradiation. Somewhat surprisingly, single-cell observations revealed that virtually all multinucleated giant cells that remain adherent for the duration of the experiments (up to three weeks post-irradiation) retain viability and metabolize 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), and the majority (>60%) exhibit DNA synthesis. We further report that treatment of multinucleated giant cells with pharmacological activators of apoptosis (e.g., sodium salicylate) triggers their demise. Our observations reinforce the notion that radiation-induced multinucleation may reflect a survival mechanism for p53/p21-deficient cancer cells. With respect to evaluating radiosensitivity, our observations underscore the importance of single-cell experimental approaches (e.g., single-cell MTT) as the creation of viable multinucleated giant cells complicates the interpretation of the experimental data obtained by commonly-used multi-well plate colorimetric assays.

show abstract

“…PrestoBlue, like AlamarBlue, is a resazurin‐based cell viability indicator recently indicated as a fast, sensitive viability assay for a broad range of cell types including bacterial . In viable cells, aerobic respiration reduces nonfluorescent resazurin to fluorescent resorufin, inducing PrestoBlue to fluoresce and indicating viability . Here, MRSA, VRE, S. warneri , and B. subtilis were susceptible to the dendrimers, becoming nonviable as the concentration of the dendrimers increased (Figures A,B).…”

Section: Resultsmentioning

confidence: 99%

“…We, therefore, used PrestoBlue cell viability assay to assess the susceptibility of Gram‐positive MRSA, VRE, S. warneri , and B. subtilis to DEN7 and DEN9 . PrestoBlue, like AlamarBlue, is a resazurin‐based cell viability indicator recently indicated as a fast, sensitive viability assay for a broad range of cell types including bacterial . In viable cells, aerobic respiration reduces nonfluorescent resazurin to fluorescent resorufin, inducing PrestoBlue to fluoresce and indicating viability .…”

Section: Resultsmentioning

confidence: 99%

Quaternized and Thiazole‐Functionalized Free Radical‐Generating Organometallic Dendrimers as Antimicrobial Platform against Multidrug‐Resistant Microorganisms

Abd‐El‐Aziz

Agatemor

Etkin

et al. 2017

Macromolecular Bioscience

View full text Add to dashboard Cite

New macromolecules such as dendrimers are increasingly needed to drive breakthroughs in diverse areas, for example, healthcare. Here, the authors report hybrid antimicrobial dendrimers synthesized by functionalizing organometallic dendrimers with quaternary ammonium groups or 2-mercaptobenzothiazole. The functionalization tunes the glass transition temperature and antimicrobial activities of the dendrimers. Electron paramagnetic resonance spectroscopy reveals that the dendrimers form free radicals, which have significant implications for catalysis and biology. In vitro antimicrobial assays indicate that the dendrimers are potent antimicrobial agents with activity against multidrug-resistant pathogens such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium as well as other microorganisms. The functionalization increases the activity, especially in the quaternary ammonium group-functionalized dendrimers. Importantly, the activities are selective because human epidermal keratinocytes cells and BJ fibroblast cells exposed to the dendrimers are viable after 24 h.

show abstract

Use of the viability reagent PrestoBlue in comparison with alamarBlue and MTT to assess the viability of human corneal epithelial cells

Cited by 153 publications

References 21 publications

Cytotoxicity of Odorous Compounds from Poultry Manure

Cytotoxicity of Odorous Compounds from Poultry Manure

Multinucleated Giant Cancer Cells Produced in Response to Ionizing Radiation Retain Viability and Replicate Their Genome

Quaternized and Thiazole‐Functionalized Free Radical‐Generating Organometallic Dendrimers as Antimicrobial Platform against Multidrug‐Resistant Microorganisms

Contact Info

Product

Resources

About