Use of tissues embedded in epoxy resin for routine histological examination of renal biopsies

Abstract

SYNOPSIS The production of 0 4,u thick, full-length sections from renal tissue taken by needle biopsy is achieved by formalin and osmic acid fixation and embedding in epoxy resin as for ultramicrotomy. The marked improvement in histological clarity by using this technique is illustrated by comparison with material embedded in paraffin.

Demonstration of basement membrane in renal biopsies by silver-methenamine on thin epoxyresin sections.

“…They illustrate their excellent results with Jones's silver technique. In this laboratory, however, difficulty was experienced with their use of ammoniacal ethanol and as we, in agreement with Eastham and Essex (1969), believe that the thinner sections possible with polymer-embedding are of greater diagnostic value, the present note describes the successful application of a methenamine-silver method on sections from Araldite. …”

Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material.

“…The value of thin sections prepared from epoxy resin-embedded tissue for the routine histological examination of renal biopsies and other tissues was emphasized recently by Eastham and Essex (1969), thus confirming the use of thin sections for renal tissues as advocated earlier by Jones (1957a). However, most epoxy resin techniques, when compared with paraffin methods, possess several disadvantages.…”

The use of a low‐viscosity epoxy resin in the preparation of undecalcified bone sections for light microscopy

“…Vinylcyclohexene dioxide 10 g Diglycidyl ether of polypropyleneglycol 6 g Nonenyl succinic anhydride 26 g Dimethylaminoethanol 0.4 g (Obtained from Polysciences Inc., Warrington, Penna., 18976.) Sections 0.6-1 pm in thickness are cut on a Reichert OMU, ultramicrotome using a glass knife and affixed to a glass slide without the use of adhesives (Eastham & Essex, 1969). The slides are placed on a slide dryer at 60°C for 1 h and then allowed to stand at room temperature for a further 30 min.…”