Use of TLC for the Quantitative Determination of Acetylsalicylic Acid, Caffeine, and Ethoxybenzamide in Combined Tablets

Bocheńska, Paulina; Pyka, Alina

doi:10.1080/10826076.2013.790766

Cited by 9 publications

(11 citation statements)

References 5 publications

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“…The results of this study and those presented in our previous papers [39–43] indicate that the validated TLC-densitometric method can be successfully applied to the determination of biological active compounds in selected pharmaceutical formulations.…”

Section: Resultssupporting

confidence: 65%

“…Based on the results of the robustness test (Table 2, experimental design matrix (2 3 ) for robustness), which were determined according to the guidelines described in the papers by Nagy-Turák and Ferenczi-Fodor et al, it was stated that the proposed TLC-densitometric method can be regarded as robust. Moreover, it can be suggested that the modification of robustness test procedure in comparison to the methodology presented by Nagy-Turák and Ferenczi-Fodor et al [35, 37] and to our previous investigations [39–43] is suitable for robustness study of TLC-densitometric method used in acetaminophen determination in combined pharmaceutical dosage form. …”

Section: Resultsmentioning

confidence: 97%

“…The levels of factors investigated and the experimental design matrix (2 3 ) are shown in Tables 1 and 2, respectively. The ways of calculation of the effects ( E ) characterizing the particular individual factors and rank probabilities [38] were early presented [35, 37, 39–43]. …”

Section: Methodsmentioning

confidence: 99%

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Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

Pyka

Budzisz

Dołowy

2013

BioMed Research International

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Adsorption thin layer chromatography (NP-TLC) with densitometry has been established for the identification and the quantification of acetaminophen in three leading commercial products of pharmaceutical tablets coded as brand: P1 (Product no. 1), P2 (Product no. 2), and P3 (Product no. 3). Applied chromatographic conditions have separated acetaminophen from its related substances, namely, 4-aminophenol and and 4′-chloroacetanilide. UV densitometry was performed in absorbance mode at 248 nm. The presented method was validated by specificity, range, linearity, accuracy, precision, detection limit, quantitative limit, and robustness. The TLC-densitometric method was also compared with a pharmacopeial UV-spectrophotometric method for the assay of acetaminophen, and the results confirmed statistically that the NP-TLC-densitometric method can be used as a substitute method. It could be said that the validated NP-TLC-densitometric method is suitable for the routine analysis of acetaminophen in quantity control laboratories.

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Section: Resultssupporting

confidence: 65%

Section: Resultsmentioning

confidence: 97%

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Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

Pyka

Budzisz

Dołowy

2013

BioMed Research International

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“…Various analytical methods have been employed to determine the amount of CAF in many matrices, including spectroscopic methods such as spectrophotometry and spectrofluorimetry . Chromatographic separation methods have been addressed for the detection of CAF, including high‐pressure liquid chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC–MS/MS) , thin layer chromatography (TLC), gas chromatography and capillary zone electrophoresis . Furthermore, potentiometry and voltammetry as electrochemical methods have been reported for CAF detection.…”

Section: Introductionmentioning

confidence: 99%

Prospects for using a new sequential chemiluminescence strategy for monitoring the caffeine content in soft and energy drinks via the catalytic activities of different nano‐metal oxides

et al. 2019

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This article suggests a new sequential injection analysis chemiluminescence (SIA-CL) strategy for monitoring the caffeine (CAF) content in soft and energy drinks using the catalytic activities of different nano-metal oxides. The present study describes three different SIA-CL systems (luminol-ferricyanide (III) coupled with Fe 2 O 3 or ZnO nanoparticles (NPs), and luminol-H 2 O 2 coupled with CuONPs. All experimental conditions were optimized and the linear concentration ranges of pure CAF were evaluated using the calibration graphs. The selectivity of the developed SIA-CL systems was studied under the influence of various interfering species that may be present in soft or energy drinks such as sodium ions, sucrose, glucose, sodium benzoate, sodium citrate, riboflavin, niacin, citric, phosphoric and ascorbic acids. International Council for Harmonization (ICH) guidelines were obeyed for the validation of the suggested CL methods. The developed SIA-CL systems displayed linear relationships over the concentration ranges 1.0-350, 5.0-400 and 10.0-400 μg ml −1 with Fe 2 O 3 NPs, ZnO NPs and CuO NPs, respectively. The recorded lower limits of detection and quantification were 0.7, 2.7 and 7.8 μg ml −1 , and 1.0, 5.0 and 10.0 μg ml −1 for the previously mentioned SIA-CL systems. The results revealed high selectivity for CAF determination and were in good agreement with those obtained by other reported methods. KEYWORDS caffeine, chemiluminescence, energy drinksmetal nano-oxidessequential injection analysissoft drinks

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“…So far, various analytical techniques have been developed for the determination of ASA in pharmaceutical tablets such as UV-spectrophotometry [2], spectrofluorometry [3], HPLC (high-performance liquid chromatography) [4], and TLC (thin-layer chromatography) [3,5,6,7,8]. The method recommended by Polish and United States Pharmacopoeias for ASA is HPLC with UV detection [9,10].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Cost-Effective TLC-Densitometric Method for the Quantitative Determination of Acetylsalicylic Acid and Ascorbic Acid in Combined Effervescent Tablets

et al. 2018

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A new, simple, and cost-effective TLC-densitometric method has been established for the simultaneous quantitative determination of acetylsalicylic acid and ascorbic acid in combined effervescent tablets. Separation was performed on aluminum silica gel 60F254 plates using chloroform-ethanol-glacial acid at a volume ratio of 5:4:0.03 as the mobile phase. UV densitometry was performed in absorbance mode at 200 nm and 268 nm for acetylsalicylic acid and ascorbic acid, respectively. The presented method was validated as per ICH guidelines by specificity, linearity, accuracy, precision, limit of detection, limit of quantification, and robustness. Method validations indicate a good sensitivity with a low value of LOD and LOQ of both examined active substances. The linearity range was found to be 1.50–9.00 μg/spot and 1.50–13.50 μg/spot for acetylsalicylic and ascorbic acid, respectively. A coefficient of variation that was less than 3% confirms the satisfactory accuracy and precision of the proposed method. The results of the assay of combined tablet formulation equal 97.1% and 101.6% in relation to the label claim that acetylsalicylic acid and ascorbic acid fulfill pharmacopoeial requirements. The developed TLC-densitometric method can be suitable for the routine simultaneous analysis of acetylsalicylic acid and ascorbic acid in combined pharmaceutical formulations. The proposed TLC-densitometry may be an alternative method to the modern high-performance liquid chromatography in the quality control of above-mentioned substances, and it can be applied when HPLC or GC is not affordable in the laboratory.

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Use of TLC for the Quantitative Determination of Acetylsalicylic Acid, Caffeine, and Ethoxybenzamide in Combined Tablets

Cited by 9 publications

References 5 publications

Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method

Prospects for using a new sequential chemiluminescence strategy for monitoring the caffeine content in soft and energy drinks via the catalytic activities of different nano‐metal oxides

A Simple and Cost-Effective TLC-Densitometric Method for the Quantitative Determination of Acetylsalicylic Acid and Ascorbic Acid in Combined Effervescent Tablets

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