“…In plants, Sandery et al (1991) first applied the microdissection technique toward isolating B-chromosomes from rye ( Secale cereale Linnaeus, 1753) and were able to identify a DNA sequence on these rye B-chromosomes. With the development of PCR, microdissection techniques have widely been used with genetic studies of Secale cereale ( Houben et al 1996 ; Zhou et al 1999 ), Triticum aestivum Linnaeus, 1753 ( Hu et al 2004 ), Zea mays Linnaeus, 1753 ( Stein et al 1998 ), Avena sativa Linnaeus, 1753 ( Chen and Armstrong 1995 ; Sanz et al 2012 ), Gossypium arboreum Linnaeus, 1753 ( Renhai et al 2012 ), Citrus grandis Osbeck, 1757 ( Huang et al 2004a , b ), Silene latifolia Poiret, 1789 ( Hobza et al 2004 , 2007 ), Populus tremula Linnaeus, 1753 ( Zhang et al 2005 ), an addition line of wheat- Thinopyrum intermedium Barkworth & Dewey, 1985 ( Deng et al 2013a ) and Spinacia oleracea Linnaeus, 1753 ( Deng et al 2013b ). Chromosome microdissection and cloning are powerful tools that combine cytogenetics with molecular genetics and have played an important role in research on genome structure ( Fominaya et al 2005 ; Hobza and Vyskot 2007 ).…”