Use of ultrasonic pachymetry for measurement of changes in corneal thickness in mouse corneal transplant rejection

Abstract

In vivo graft pachymetry provides a new and reliable way to objectively diagnose rejection in the mouse model of corneal transplantation.

Use of Anterior Segment Optical Coherence Tomography to Predict Corneal Graft Rejection in Small Animal Models

“…6,10,11 Compared to larger animal models, rats are genetically identically inbred, allowing control for many experimental variables. 5 In the present study, we demonstrated that moderate to severe AC inflammation during the early stage after PK could predict graft failure earlier than other indicators. In the rat model, eyes with grade 3 or higher AC inflammation at 1 week or with ‡400 lm central graft thickness at 2 weeks (normal rat corneal thickness, 170 lm), were significant predictors of graft failure at 4 weeks.…”

“…Ultrasonic pachymetry has been evaluated as a possible method for determining allograft rejection in a recent clinical trial, 12 and corneal thickness has been reported to serve as a predictor of graft survival. 16 Flynn et al 5 reported that in vivo graft ultrasound pachymetry provided a reliable way to objectively diagnose rejection in a mouse model of corneal transplantation. Compared to ultrasonic pachymetry, ASOCT is sterile and noncontact, providing more in-depth imaging and micrometric measurements for corneal thickness.…”

“…4 However, this is graded subjectively, and it can be difficult, especially in cases of mild edema or when the severity of graft opacity or neovascularization varies in different regions of the transplanted cornea. 5 In our previous study using a rat PK model, 6 we also encountered this problem in diagnosing graft rejection. Flynn et al, 5 therefore, reported the use of in vivo graft pachymetry as a useful tool to objectively diagnose graft rejection in a mouse model of corneal transplantation.…”

“…5 In our previous study using a rat PK model, 6 we also encountered this problem in diagnosing graft rejection. Flynn et al, 5 therefore, reported the use of in vivo graft pachymetry as a useful tool to objectively diagnose graft rejection in a mouse model of corneal transplantation.…”

The effect of perioperative allergic conjunctivitis on corneal lymphangiogenesis after corneal transplantation

“…Corneal transplantation and diagnosis of graft rejection were performed as previously described 16. Briefly, recipient mice were anaesthetised with an i.p.…”