Use of Ultraviolet Light and Fluorescent Dyes in Bacterial Counting

Natural and Induced Fluorescence in Microscopic Organisms

“…These may be standard, colored dyes, which visibly stain the specimen and which, on irradiation, fluoresce with a similar or dissimilar color; other fluors remain colorless until irradiated. Some of the uses of fluors include labeling for localization of antigen in tissue cells (Coons and Kaplan, 1950;Coons et al, 1942); labeling for bacterial and viral infiltration (Hagemann, 1937;Maximovick and Mitchenko, 1959;Rapp, Gordon, and Baker, 1960); discrimination of certain types of cancer cells (Bertalanffy, 1960;Masin, 1956, 1958;Friedman, 1950); identification of specific bacterial genera (Kaufman and Weaver, 1960); studies on bone structure and development (Ribelin, Masri, and Deeds, 1960); staining techniques for tubercle bacilli (Hagemann, 1937;Kuper and May, 1960), trypanosomes (Jansco, 1932;Strugger, 1948a), and malarial organisms (Fishl and Singer, 1935); bacterial counts (Greene and Hesseltine, 1950;Strugger, 1948b); localization of inhaled dust (Yogoda and Donahue, 1946); and detection of antibiotics in milk (Hargrove, Lehman and Matthews, 1958).…”