Use of vitamin C in delayed tooth replantation

Abstract

This study evaluated microscopically the effects of root surface treatment with three different solutions in delayed rat teeth replantation. Central incisors from 30 rats (Rattus norvegicus, albinus Wistar) were extracted and left on a bench for 6 h. The pulps were extirpated and root canals were irrigated with 1% sodium hypochlorite. After endodontic treatment, the root surfaces of all teeth were submitted to a 10-min treatment with 1% sodium hypochlorite, changed every 5 min. The teeth were then rinsed with saline for 10 min and assigned to 3 groups with ten specimens each. Groups I, II and III were treated, respectively, with 2% acidulated-phosphate sodium fluoride, vitamin C solution and effervescent vitamin C (2 g, Redoxon ® ). After root surface treatment, the teeth were filled with calcium hydroxide and replanted. The animals were sacrificed after 10 and 60 days. Group I (fluoride) presented the largest areas of replacement resorption and ankylosis. Comparing both vitamin C groups, Group III (effervescent vitamin C) yielded better results, showing more areas of ankylosis and replacement resorption than areas of inflammatory resorption.

Delayed tooth replantation after treatment of necrotic periodontal ligament with citric acid

“…Most studies referring to the use of acidic substances (hydrochloric, citric, phosphoric and ascorbic acids) in cases of delayed tooth replantation have investigated the repair process after complete PDL removal by mechanical or chemical means and/or the treatment of root surface with the abovementioned acid solutions [7][8][9][10][11][12]. However, the biocompatibility of the necrotic periodontal ligament treated with citric acid is unknown.…”

“…Acidic solutions, such as citric acid, phosphoric acid, hydrochloric acid and ascorbic acid have been employed for root surface treatment after removal of periodontal ligament [7][8][9][10][11][12]. The goals of using acidic solutions include pH neutralization (if sodium hypochlorite is used for chemical removal of periodontal ligament), reattachment of collagen fibers to cementum surface [7][8][9][10][11][12][13] and elimination of bacterial endotoxin [14].…”

Treatment of root surface in delayed tooth replantation: a review of literature

“…This can be performed either mechanically by scraping with curettes (20,(23)(24)(25)(26)(27), scalpel blade (17,28,29), rubber cup polishing with pumice/water slurry (30), diamond bur and sandpaper disk (31,32) or chemically with sodium hypochlorite (21,22,(33)(34)(35). This can be performed either mechanically by scraping with curettes (20,(23)(24)(25)(26)(27), scalpel blade (17,28,29), rubber cup polishing with pumice/water slurry (30), diamond bur and sandpaper disk (31,32) or chemically with sodium hypochlorite (21,22,(33)(34)(35).…”

“…Among the fluoride solutions, the use of 2% acidulated sodium phosphate fluoride has shown a decrease in inflammatory root resorption and the predominance of areas of ankylosis and replacement resorption (23,33,36,37,48). Fluoride probably acts directly on the bone tissue, cementum and dentin, by converting hydroxyapatite into fluorapatite, or by a specific inhibitory action on the clastic cells, or even an association of both hypotheses (38).…”

“…The best results have been achieved with lower concentrations and consisted predominantly of long-term replacement resorption and ankylosis and short-term absence of resorption (21,22,33,34). Therefore, the removal of necrotic periodontal ligament remnants has been recommended in an attempt to increase the survival rate of replanted teeth.…”