Use of voltage-sensitive dyes and optical recordings in the central nervous system

Ebner, Timothy J.; Chen, Gang

doi:10.1016/0301-0082(95)00010-s

Cited by 195 publications

(102 citation statements)

References 186 publications

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“…Both extensive lateral cortico-cortical connections (35,40) and diffuse nonlemniscal inputs (32) might contribute to this significant lateral spread of neuronal activity. Because voltage-sensitive dye measurements are sensitive to subthreshold neuronal activity (41,42), this observation also advocates a significant contribution of subthreshold synaptic activity to hemodynamic signals.…”

Section: Discussionmentioning

confidence: 93%

Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity

Devor

Ulbert

Dunn

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

214

180

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Accurate interpretation of functional MRI (fMRI) signals requires knowledge of the relationship between the hemodynamic response and the neuronal activity that underlies it. Here we address the question of coupling between pre-and postsynaptic neuronal activity and the hemodynamic response in rodent somatosensory (Barrel) cortex in response to single-whisker deflection. Using full-field multiwavelength optical imaging of hemoglobin oxygenation and electrophysiological recordings of spiking activity and local field potentials, we demonstrate that a point hemodynamic measure is influenced by neuronal activity across multiple cortical columns. We demonstrate that the hemodynamic response is a spatiotemporal convolution of the neuronal activation. Therefore, positive hemodynamic response in one cortical column might be explained by neuronal activity not only in that column but also in the neighboring columns. Thus, attempts at characterizing the neurovascular relationship based on point measurements of electrophysiology and hemodynamics may yield inconsistent results, depending on the spatial extent of neuronal activation. The finding that the hemodynamic signal observed at a given location is a function of electrophysiological activity over a broad spatial region helps explain a previously observed increase of local vascular response beyond the saturation of local neuronal activity. We also demonstrate that the oxy-and total-hemoglobin hemodynamic responses can be well approximated by space-time separable functions with an antagonistic center-surround spatial pattern extending over several millimeters. The surround ''negative'' hemodynamic activity did not correspond to observable changes in neuronal activity. The complex spatial integration of the hemodynamic response should be considered when interpreting fMRI data.Barrel cortex ͉ blood oxygenation ͉ intrinsic signals ͉ optical imaging T he advent of noninvasive imaging methods such as functional MRI (fMRI) has made it possible to obtain spatial maps of hemodynamic ''activation'' in the human brain under a variety of conditions (1, 2). However, the indirect and poorly understood nature of the coupling between these hemodynamic signals and the underlying neuronal activity has greatly limited the interpretability of neuroimaging results. Recently, several groups have attempted to characterize this coupling in the form of a linear or nonlinear neurovascular ''transfer function '' (3-8). In principle, if such a function could be defined, it would provide a basis for inferring time-averaged local neuronal activity based on hemodynamic measurements. Furthermore, it would permit more accurate integration of hemodynamic imaging methods with noninvasive electrophysiological recordings such as electroencephalography and magnetoencephalography (9, 10).In a previous publication (3), using simultaneous spectroscopic optical imaging and electrophysiological measurements in rodent somatosensory cortex during brief and spatially localized stimuli, we found a strongly nonlinear r...

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Section: Discussionmentioning

confidence: 93%

Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity

Devor

Ulbert

Dunn

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

214

180

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“…For those techniques, waking animal experiments that require the animal to be trained to tolerate restraint in a holder and experimental set-up have been established (Berwick et al, 2002;Martin et al, 2002). Compared with other imaging methods, one major advantage that optical imaging techniques offer in neurovascular coupling studies is that they allow for imaging of neural activity on a mesoscopic scale similar to that of the hemodynamic imaging accomplished by measuring activity-induced changes in membrane potentials (Obrenovitch et al, 2009;Takashima et al, 2001;Ebner and Chen, 1995), intracellular pH (Sun et al, 2011), calcium ion concentrations (Homma et al, 2009) combined with exogenous fluorescent compounds, and endogenous fluorescent changes arising from activity-dependent cellular autofluorescence (Reinert et al, 2007;Shibuki et al, 2003). These techniques enable us to directly examine the spatial gaps between neural and vascular response regions (Weber et al, 2004).…”

Section: Why Is Anesthesia Needed?mentioning

confidence: 99%

Anesthesia and the Quantitative Evaluation of Neurovascular Coupling

Masamoto

Kanno²

2012

J Cereb Blood Flow Metab

245

191

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Anesthesia has broad actions that include changing neuronal excitability, vascular reactivity, and other baseline physiologies and eventually modifies the neurovascular coupling relationship. Here, we review the effects of anesthesia on the spatial propagation, temporal dynamics, and quantitative relationship between the neural and vascular responses to cortical stimulation. Previous studies have shown that the onset latency of evoked cerebral blood flow (CBF) changes is relatively consistent across anesthesia conditions compared with variations in the time-to-peak. This finding indicates that the mechanism of vasodilation onset is less dependent on anesthesia interference, while vasodilation dynamics are subject to this interference. The quantitative coupling relationship is largely influenced by the type and dosage of anesthesia, including the actions on neural processing, vasoactive signal transmission, and vascular reactivity. The effects of anesthesia on the spatial gap between the neural and vascular response regions are not fully understood and require further attention to elucidate the mechanism of vascular control of CBF supply to the underlying focal and surrounding neural activity. The in-depth understanding of the anesthesia actions on neurovascular elements allows for better decision-making regarding the anesthetics used in specific models for neurovascular experiments and may also help elucidate the signal source issues in hemodynamic-based neuroimaging techniques.

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“…Because the turtle Cb is thin, a novel approach is developed here to simultaneously record physiological responses across the entire Cb surface using voltage-sensitive dye recording techniques developed by Salzberg and Grinvald (Baker et al 2005; see modern reviews by Ebner and Chen 1995;Salzberg et al 1977;Wu et al 1998). This technique permits us to measure physiological activity with submillisecond resolution from a two-dimensional sheet of cortical tissue during afferent stimulation.…”

Section: Introductionmentioning

confidence: 99%

Topography and Response Timing of Intact Cerebellum Stained With Absorbance Voltage-Sensitive Dye

Brown¹,

Ariel²

2009

Journal of Neurophysiology

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Use of voltage-sensitive dyes and optical recordings in the central nervous system

Cited by 195 publications

References 186 publications

Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity

Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity

Anesthesia and the Quantitative Evaluation of Neurovascular Coupling

Topography and Response Timing of Intact Cerebellum Stained With Absorbance Voltage-Sensitive Dye

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