Use of wounds in the parietal bone of the rat for evaluating bone marrow for grafting into periodontal defects

Turnbull, Robert S.; Freeman, Eric

doi:10.1111/j.1600-0765.1974.tb00651.x

Cited by 35 publications

(22 citation statements)

References 11 publications

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“…CSDs in the calvaria have been established for different mammalian species [9][10][11][12][13][14]. Modelling on parietal bone has been applied on rats from different strains and at various ages [10,[15][16][17][18]. Three months old Sprague-Dawley rats did not spontaneously fill 8 mm diameter lacunae 18 weeks post-surgery [6].…”

Section: Introductionmentioning

confidence: 99%

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

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Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promote bone repair in vivo when combined with porous scaffolds based on poly(L-lactic acid) (PLA) obtained by supercritical gas foaming and reinforced with 5 wt.% β-tricalcium phosphate (TCP).Bone regeneration was assessed by radiography and histology after implantation of PLA/TCP scaffolds alone, seeded with primary fetal bone cells, or coated with demineralized bone matrix. Craniotomy critical size defects and drill defects in the femoral condyle in rats were employed. In the cranial defects, polymer degradation and cortical bone regeneration were studied up to 12 months postoperatively. Complete bone ingrowth was observed after implantation of PLA/TCP constructs seeded with human fetal bone cells. Further tests were conducted in the trabecular neighborhood of femoral condyles, where scaffolds seeded with fetal bone cells also promoted bone repair.We present here a promising approach for bone tissue engineering using human primary fetal bone cells in combination with porous PLA/TCP structures. Fetal bone cells could be selected regarding osteogenic and immune-related properties, along with their rapid growth, ease of cell banking and associated safety.

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Section: Introductionmentioning

confidence: 99%

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Montjovent

Mark

Mathieu

et al. 2008

Bone

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“…One of the factors affecting bone repair is defect size 18) , and a critical size defect is an experimental defect where natural osseous healing does not occur 19) . Freeman and Turnbull prepared 2-mm bone defects in the temporal bone of albino Wistar rats weighing 500 g and reported that the defects were not repaired by new bone even after 12 weeks 20,21) .…”

Section: Experimental Bone Defectmentioning

confidence: 99%

Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats

Togari

Miyazawa

Yagihashi

et al. 2012

J. Hard Tissue Biology.

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Alveolar bone defects may result in functional and morphological abnormalities. In such cases, bone can be regenerated by grafting fresh autogenous bone into the defect. Subsequently, we focused on the dentin of third molars, which are usually discarded after extraction, or teeth removed faor orthodontic therapy. As in bone, type I collagen is the main component of the organic matrix in dentin. Also, it has been proven that the organic matrix of dentin contains osteoinductive bone morphogenetic protein (BMP). It would be safe to perform grafting with the dentin of extracted teeth used as an autogenous bone substitute. Therefore, the objective of the present study was to reuse extracted teeth as a bone substitute material. Demineralized dentin matrix (DDM), an organic material derived from the dentine of bovine teeth, was grafted in rat skull defects to determine its bone regeneration ability and the possibility of its use as a bone substitute. In the present study, new bone formed from the defect margin in the control group, but in the graft group, new bone formation occurred from individual DDM granules within the defect, and not just from the margin. At Week 8, defect repair was limited in the control group. In contrast, most of the defect was covered by osseous tissue in the graft group. In conclusion, DDM may be a useful bone substitute that serves as a scaffold for bone regeneration by inducing a high level of new bone formation soon after surgery.

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“…21 Modeling on parietal bone has been applied to rats from different strains and at various ages. [7][8][9]22,23 The SpragueDawley rats used herein did not spontaneously fill 8 mm diameter full thickness defects 12-weeks postsurgery. 24 Different types of bone graft substitutes can be used to fill a CSD.…”

Section: Introductionmentioning

confidence: 99%

“…4 CSDs in the calvaria have been established for different animal species. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] The rat calvarial model offers advantages: the parietal bone is a large plate that facilitates the operation and the analysis (histology, imaging), no implant fixation is required, and the costs are limited in comparison with large animal models. Furthermore, as this experimental approach is widely used, a precise comparison of different grafted materials is possible.…”

Section: Introductionmentioning

confidence: 99%

Repair of critical size defects in the rat cranium using ceramic‐reinforced PLA scaffolds obtained by supercritical gas foaming

Montjovent

Mathieu

Schmoekel

et al. 2007

J Biomedical Materials Res

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Bioresorbable scaffolds made of poly(L-lactic acid) (PLA) obtained by supercritical gas foaming were recently described as suitable for tissue engineering, portraying biocompatibility with primary osteoblasts in vitro and interesting mechanical properties when reinforced with ceramics. The behavior of such constructs remained to be evaluated in vivo and therefore the present study was undertaken to compare different PLA/ceramic composite scaffolds obtained by supercritical gas foaming in a critical size defect craniotomy model in Sprague-Dawley rats. The host-tissue reaction to the implants was evaluated semiquantitatively and similar tendencies were noted for all graft substitutes: initially highly reactive but decreasing with time implanted. Complete bonebridging was observed 18 weeks after implantation with PLA/ 5 wt % b-TCP (PLA/TCP) and PLA/5 wt % HA (PLA/HA) scaffolds as assessed by histology and radiography. We show here for the first time that this solvent-free technique provides a promising approach in tissue engineering demonstrating both the biocompatibility and osteoconductivity of the processed structures in vivo.

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Use of wounds in the parietal bone of the rat for evaluating bone marrow for grafting into periodontal defects

Cited by 35 publications

References 11 publications

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats

Repair of critical size defects in the rat cranium using ceramic‐reinforced PLA scaffolds obtained by supercritical gas foaming

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