Use of X-Irradiations in Reducing the Waste of Aflatoxin-Contaminated Pistachios and Evaluation of the Physicochemical Properties of the Irradiated Product

Abstract

This study investigates the effects of electron beam irradiation (0, 1, 2, 4, and 6 kGy) on Aspergillus flavus, aflatoxin B1 (AFB1), and the physicochemical properties of pistachios. The findings suggested that e-beam significantly reduced the spore population of A. flavus and the concentration of AFB1 at doses of 4 and 6 kGy. Three AFB1 degradation products were detected via LC-MS analysis and their structures were presented. Total phenolic content was improved at a dose of 2 kGy, while antioxidant activity was decreased in all treatments in both DPPH and ABTS assays. The chlorophyll and carotenoid content declined and the color indices changed, leading to a darker color. E-beam at a dose of 2 kGy raised the soluble protein levels and changed the intensity and pattern of protein bands. Irradiation doses of up to 6 kGy enhanced the content of malondialdehyde and total saturated fatty acids while leading to a decline in unsaturated fatty acids. The quality features were adversely affected at doses > 4 kGy. The findings suggest that as an alternative method, e-beam at doses ≥ 2 kGy can effectively decrease fungal load and aflatoxin B1 contamination, and e-beam application at doses ≤ 2 kGy can maintain the physicochemical attributes of pistachios to an acceptable extent.

A New Method for Extraction of Aflatoxin B1 from Nuts for Competitive Enzyme-linked Immuno Assay

“…The sample extract is filtered, diluted with water and injected into an affinity chromatography column containing antibodies specific for aflatoxins B1, B2, G1, G2. Aflatoxins are quantified using reverse phase high-performance liquid chromatography (HPLC) with fluorescence detection and post-column derivatization [37,38].…”