Usefulness of a commercial enzyme-linked immunosorbent assay kit for Candida mannan antigen for detecting Candida in oral rinse solutions

Kitamura, Hiroshi; Kamata, Toshihide; Zhao, Chen yao; Narikawa, Jun-nosuke; Koike, Takeshi; Kurashina, Kenji

doi:10.1016/j.tripleo.2008.12.025

Cited by 12 publications

(8 citation statements)

References 10 publications

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“…The sensitivity and of Candida antigen detection in the present study was similar to previous study (Alam et al, 2007) and it was less than that reported by Kurita et al (2009). While the specificity was higher than that previously reported (Badiee et al, 2014).…”

Section: Mannan Antigen Detectionsupporting

confidence: 83%

“…While the specificity was higher than that previously reported (Badiee et al, 2014). The difference in sensitivity may be attributed to the fact that mannan antigen of Candida species is rapidly cleared from the patient's blood (Kurita et al, 2009) The study tried to detect the best cutoff value for quantitative detection of mannan antigen. This increases the sensitivity of the test with lowering the specificity.…”

Section: Mannan Antigen Detectionmentioning

confidence: 83%

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Evaluation of semi-nested polymerase chain reaction (PCR) and mannan antigen detection compared to blood culture for diagnosis of candidemia

Al-Kasaby¹,

Kheir²,

Mefreh³

et al. 2017

Afr. J. Microbiol. Res.

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Invasive Candida infections have emerged as an important pathogen in the last decade, especially in immunocompromized patients. The aim of the present study was to evaluate the detection of Candida species in blood samples from pediatric patients with sepsis by blood culture method versus antigen detection method by enzyme linked immunosorbent assay (ELISA) and molecular method by polymerase chain reaction (PCR). This cross-sectional study was carried out on children at Mansoura University Children Hospital (MUCH) with the presence of clinical signs suggesting sepsis with absence of prior antifungal therapy. Laboratory diagnosis included blood culture, mannan antigen detection of Candida species by ELISA and semi-nested PCR. Candida species were detected by blood culture in 15.6% of the children and was detected by PCR and antigen detection in 20% for each. Candidemia was more frequently detected among patients with central venous lines (38.5%), children with Diabetes Mellitus (DM) (23.1%) and children with frequent blood transfusions (15.4%). However, these risk factors were not statistically significant. Mortality rate among children with candidemia was 42.9%. The sensitivity, specificity, and accuracy of antigen detection method by ELISA compared to culture, were 71.4, 89.5 and 86.7%, respectively. The sensitivity, specificity and accuracy of semi-nested PCR compared to culture were 85.7, 92.1 and 91.1% respectively. It can be concluded from this study that Candida species is a frequent pathogen associated with pediatric sepsis. Blood culture though is a reliable laboratory method for its diagnosis may lack sensitivity and requires prolonged time. Seminested PCR for detection of candidemia is sensitive, specific and accurate method. Mannan antigen detection by ELISA is rapid and easy; however, it may lacks specificity; it can be used as a preliminary method for screening. Further studies are recommended to detect the appropriate laboratory algorithm for early diagnosis of candidemia to start antifungal therapy appropriately.

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Section: Mannan Antigen Detectionsupporting

confidence: 83%

Section: Mannan Antigen Detectionmentioning

confidence: 83%

Evaluation of semi-nested polymerase chain reaction (PCR) and mannan antigen detection compared to blood culture for diagnosis of candidemia

Al-Kasaby¹,

Kheir²,

Mefreh³

et al. 2017

Afr. J. Microbiol. Res.

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“…To date, saliva samples for clinical diagnosis of fungal infections are only used for pathogen isolation and not for direct clinical assay applications. Yet, the performance of a commercial enzyme-linked immunosorbent assay (ELISA) kit to detect Candida’s mannan antigen in oral rinse solutions has been reported, but further assay optimization is needed for oral specimens [94]. Experimental attempts have also been made to detect salivary IgA or IgG antibodies to Candida [95, 96, 97], but immunodiagnosis remains elusive because of differences observed in sensitivity and specificity of different assays when detecting various Candida antigen preparations.…”

Section: Salivary Diagnostics Of Common Oral Diseasesmentioning

confidence: 99%

Saliva as a Diagnostic Fluid

Malamud

2011

Dental Clinics of North America

402

303

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“…The mannan antigen test is used to detect systemic candidiasis [27]. The sensitivity and specificity of the MN test were reported to be 90.9% and 46.2%, respectively [28]. Only one patient in the present study was diagnosed with Candida IFR on the basis of the culture results, whereas the results of the mannan antigen test were negative for all patients.…”

Section: Discussionmentioning

confidence: 65%

Comparing immunological and molecular tests with conventional methods in diagnosis of acute invasive fungal rhinosinusitis

Badiee

Moghadami

Rozbehani

2016

J Infect Dev Ctries

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Introduction: This study compared immunological and molecular tests with conventional methods in the diagnosis of acute invasive fungal rhinosinusitis. Methodology: All tissue samples from maxillary sinuses were cultured and stained using periodic acid-Schiff. Two blood samples were cultured by bedside inoculation into BACTEC medium. Diagnostic tests were used: enzyme-linked immunosorbent assay for detection of galactomannan and mannan in serum samples, real-time PCR assays for Aspergillus spp. and Candida spp., and nested PCR for the Mucoraceae family. Results: Among 31 immunocompromised patients, based on host factors, clinical, radiological and mycological findings, 18 patients were diagnosed with documented infection. By direct smear examination, 9 patients (50%) had positive results for Mucoraceae family, but only 5 samples showed growth in the culture (55.5%). Nested PCR results for species in this family were negative in all serum samples from patients. In addition, 9 patients had positive direct microscopic findings, of which 7 specimens produced positive growth for Aspergillus flavus (77.7%). The galactomannan test was positive in 6/9 (66.6%), and Aspergillus PCR were positive in 6 patients (66.6%). The isolated agent was C. albicans in one patient. The mannan antigen test to detect Candida was negative. None of the blood cultures was positive for fungal infection. Conclusions: The efficient method to diagnose fungal rhinosinusitis was direct microscopic examination of tissue samples. Immunological and molecular methods, which are available for some important fungi, can help clinicians with the diagnosis and management of infections in patients in critical condition when tissue sampling is not available.

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Usefulness of a commercial enzyme-linked immunosorbent assay kit for Candida mannan antigen for detecting Candida in oral rinse solutions

Cited by 12 publications

References 10 publications

Evaluation of semi-nested polymerase chain reaction (PCR) and mannan antigen detection compared to blood culture for diagnosis of candidemia

Evaluation of semi-nested polymerase chain reaction (PCR) and mannan antigen detection compared to blood culture for diagnosis of candidemia

Saliva as a Diagnostic Fluid

Comparing immunological and molecular tests with conventional methods in diagnosis of acute invasive fungal rhinosinusitis

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