Usefulness of Basic Fibroblast Growth Factor (bFGF) Loaded Dissolving Microneedles for Local Therapy of Skin Wounds

Takada, Kanji; Ito, Yoshiaki; Matsumoto, Kengo; Sato, Yuka; Nishio, Maimi; Tadano, Yurie; Kamei, Yuri; Takemura, Yutaro; Inoue, Nana; Akasaka, Y.; Ono, Isao

doi:10.4236/jbnb.2013.43032

Cited by 5 publications

(6 citation statements)

References 26 publications

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“…However, there has been little interest in them for the treatment of chronic wounds and burns. In one example, Takeda et al developed microneedles using chondroitin sulfate as the base material loaded with basic fibroblast growth factor (bFGF), a growth factor that is released during the early stages of wound healing and triggers endothelial cells to exert behavior typical for wound healing processes [209]. The needle arrays were tested on rat models with wounds inflicted using a surgical scalpel.…”

Section: Passive Drug Delivery Systemsmentioning

confidence: 99%

“…The needle arrays were tested on rat models with wounds inflicted using a surgical scalpel. ELISA assays on bFGF levels in the tissue showed initially elevated concentrations that slowly declined over time [209]. Caffarel et al developed a dissolving microneedle system incorporating a photosensitizing compound that has antimicrobial effects to treat infected wounds [210].…”

Section: Passive Drug Delivery Systemsmentioning

confidence: 99%

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Drug delivery systems and materials for wound healing applications

Saghazadeh

Rinoldi

Schot

et al. 2018

Advanced Drug Delivery Reviews

619

434

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Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug delivery systems. In this review, we will first discuss the pathophysiology of chronic wounds and then the materials used for engineering drug delivery systems. Different passive and active drug delivery systems used in wound care will be reviewed. In addition, the architecture of the delivery platform and its ability to modulate drug delivery are discussed. Emerging technologies and the opportunities for engineering more effective wound care devices are also highlighted.

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Section: Passive Drug Delivery Systemsmentioning

confidence: 99%

Section: Passive Drug Delivery Systemsmentioning

confidence: 99%

Drug delivery systems and materials for wound healing applications

Saghazadeh

Rinoldi

Schot

et al. 2018

Advanced Drug Delivery Reviews

619

434

View full text Add to dashboard Cite

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“…Multiple methods for MN-mediated drug administration are used, including solid, coated, hollow, hydrogel-forming, and soluble MNs [7]. MNs have been investigated in the following areas: (a) antimicrobial wound therapy (b) endothelial cell [8][9] proliferation, c) scar repair [10][11] and (d) the treatment of chronic wounds [12][13][14] and burns over various physiological time scales related to wound type and wound healing [15][16]. Examples include biointerfacing, wound detection, and smart bandages that integrate detection and distribution [17][18].…”

Section: Introductionmentioning

confidence: 99%

Stereolithography (SLA) 3D Printing Technology in Microneedles

Kaur

2021

JPRI

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Many of the drugs show enzymatic degradation in gastrointestinal tract (GIT) or they show difficulty in permeation. In these cases, microneedles (MN) based transdermal drug delivery system offers attractive alternative to conventional needle-based and oral drug delivery systems. Microneedle drug delivery system consists of an arrangement of micrometric arrays which can be formulated with the use of different polymers and technologies. This present review is related to manufacturing of biocompatible microneedles” formulated with an aid of stereolithography (SLA) - a 3D printing technique in which microneedle patches of different shapes are constructed in the form of layers. The MN patches could be coated using inkjet printing. An SLA printer could be employed to print pyramid needle-based arrays. X-ray computer micro tomography (CT) and scanning electronic microscopy (SEM) could be used to assess the standard of the formed microneedles and subsequent coatings. In vitro studies using Franz diffusion cells could be done further to analyze drug permeation rate and calculation of flux. Microneedles could be constructed by using a 3D printing stereolithographic technology, and combining it with a highly appropriate coating method like inkjet printing, which can lead to a high-paced drug delivery microneedle systems via skin.

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“…Across multiple physiological time scales associated with wound type and wound healing [ 17 ] the use of MNs has been investigated in the following areas: (a) antimicrobial wound treatment [ 18 , 19 , 20 ]; (b) proliferation of endothelial cells [ 21 , 22 ]; (c) scar repair [ 22 , 23 ]; and, (d) treatment of chronic wounds and burns [ 24 , 25 ]. Related cutting edge applications of MNs include biointerfacing [ 26 ], wound detection [ 27 ], and smart bandages under development combining detection and delivery [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

et al. 2018

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Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant difference between the viability of the sheared and control samples for extrusion times of 2 h (p = 0.14, α = 0.05) and 24 h (p = 0.5, α = 0.05) post-atomization. Factoring the increase in extrusion yield from 21.2% to 56.4% attributed to hydrogel bioerosion quantifiable by a loss in resilience from 5470 (J/m3) to 3250 (J/m3), there was no significant difference in percentage relative payload (p = 0.2628, α = 0.05) when extrusion occurred 24 h (12.2 ± 4.9%) when compared to 2 h (9.9 ± 2.8%) post-atomization. Results from this paper highlight the feasibility of encapsulated cell extrusion, specifically protection from shear, through a hollow microneedle assembly reported for the first time in literature.

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Usefulness of Basic Fibroblast Growth Factor (bFGF) Loaded Dissolving Microneedles for Local Therapy of Skin Wounds

Cited by 5 publications

References 26 publications

Drug delivery systems and materials for wound healing applications

Drug delivery systems and materials for wound healing applications

Stereolithography (SLA) 3D Printing Technology in Microneedles

Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

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