Usefulness of collagen gel droplet embedded culture drug sensitivity testing in ovarian cancer

Yabushita, Hiromitsu; Ohnishi, Masafumi; Komiyama, Megumi; Mori, Takeshi; Noguchi, M.; Kishida, Tameko; Noguchi, Yasuyuki; Sawaguchi, K; Noguchi, Masayoshi

doi:10.3892/or.12.2.307

Cited by 10 publications

(11 citation statements)

References 14 publications

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“…In addition, Takamura et al [18] showed the CD-DST had good predictive value for the clinical response in breast cancer patients, especially for chemotherapy using TXT or some combined anticancer drugs. Several investigators [19,20] reported the clinical application of the CD-DST in gynecological cancer. Another method of in vitro chemosensitivity test is the HDRA.…”

Section: Discussionmentioning

confidence: 99%

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

et al. 2010

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“…CD-DST can mimic in vivo tumor growth using type I collagen to create a three-dimensional culture system and individually evaluate the response to chemotherapeutic drugs. Several clinical studies have reported that CD-DST may be useful when devising optimal treatment strategies for NSCLC (23), ovarian (32), gastrointestinal (33), or colon cancer (34). In patients with HNSCC, CD-DST can be also used to predict CDDP sensitivity and guide individualized chemotherapy (35).…”

Section: Discussionmentioning

confidence: 99%

Ribonucleotide reductase M2 is a promising molecular target for the treatment of oral squamous cell carcinoma

Iwamoto

Nakashiro

Tanaka

et al. 2015

International Journal of Oncology

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In our previous study, ribonucleotide reductase M2 (RRM2) was identified as a cancer-related gene commonly overexpressed in human oral squamous cell carcinoma (OSCC) cell lines. Herein, we attempted to determine whether targeting RRM2 may be a plausible therapeutic approach for the treatment of patients with OSCC. First, we examined the expression levels of RRM2 in human OSCC cell lines and tissues. Overexpression of RRM2 in OSCC was confirmed by western blot analysis. Subsequently, we investigated the effects of a synthetic small interfering RNA specific for RRM2 and gemcitabine (GEM), an inhibitor of RRM2 enzymatic activity, on the growth of human OSCC cell lines and primary cultured cells. Targeting RRM2 by RNA interference almost completely suppressed the expression of RRM2 and markedly suppressed the growth of both types of cells by >54.8%. GEM also reduced the growth rate of these cells by >83.0%. Finally, we evaluated the antitumor effects of GEM, cisplatin (CDDP), 5-fluorouracil (5-FU), and docetaxel (DOC) against OSCC cells using the collagen gel droplet embedded culture drug sensitivity test. OSCC cells were more sensitive to GEM and DOC than to CDDP and 5-FU, regardless of the expression level of RRM2 mRNA. These results suggested that RRM2 supported the growth of human OSCC cells and that targeting of RRM2, e.g., via GEM treatment, may be a promising therapeutic strategy for OSCC.

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“…Iwahashi et al reported that overall survival was significantly better in a chemosensitivity-guided chemotherapy group than in a standard chemotherapy or non-chemotherapy group in advanced gastric cancer (10). There have been several studies employing the chemosensitivity assay in ovarian cancer, breast cancer and leukemia (11)(12)(13)(14), while the number of studies using CD-DST in particular are increasing (15)(16)(17)(18)(19)(20)(21)(22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the individual 50% inhibitory area under the concentration curve of 5-fluorouracil based on the collagen gel droplet embedded culture drug sensitivity test in colorectal cancer

Ochiai¹

2009

Mol Med Rep

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Abstract.We have previously reported the 5-fluorouracil (5-FU) sensitivity of cancer cells from colorectal cancer (CRC) patients using the collagen gel droplet embedded culture-drug sensitivity test (CD-DST) under multiple drug concentrations and contact durations. Moreover, the area under the concentration curve (AUC) and growth inhibition rate (IR) were combined, resulting in the AUC-IR curve, which was approximated to the logarithmic curve. In the present study, we used the AUC-IR curve to calculate the individualized AUC IR50 , the AUC value that imparts 50% growth inhibition. Individual AUC IR50 was calculated in CRC patients, and its distribution was evaluated. The cumulative distribution of individual AUC IR50 was regressed over two lines (logarithmic scale). Among the 45 resectable CRC patients, those who achieved more than the individual AUC IR50 during post-operative 5-FUbased chemotherapy demonstrated a trend towards better disease-free survival compared to those who did not achieve AUC IR50 . Of the Dukes' D patients (n=10), those who achieved more than twice the individual AUC IR50 during post-operative 5-FU-based chemotherapy demonstrated significantly better survival rates (p=0.05) than those who did not. In this study, the distribution of the individual AUC IR50 suggested that approximately 6% of patients demonstrated very low 5-FU sensitivity. Therefore, the individual AUC IR50 was useful in classifying good, intermediate and poor 5-FU response. Achievement of the individual AUC IR50 may be a prerequisite for individualized 5-FU-based adjuvant chemotherapy. As well, the early achievement of twice the individual AUC IR50 may indicate an improved prognosis in Dukes' D patients. The individual AUC IR50 using CD-DST is useful in determining the individualized chemotherapy of CRC patients, thus CD-DST has the potential to facilitate the establishment of individualized chemotherapy for CRC. IntroductionIndividualized chemotherapy is a therapeutic strategy based on the features of a respective tumor, and has been proposed to improve patient clinical outcome while maintaining quality of life. In the field of gastrointestinal cancer, 5-fluorouracil (5-FU) is the drug most often used to assess antitumor effect. The drug sensitivity of tumor cells is one of the key factors that must be assessed in individualized chemotherapy for cancer patients. We have reported the 5-FU sensitivity of cancer cells from colorectal cancer (CRC) patients using the collagen gel droplet embedded culture-drug sensitivity test (CD-DST) under multiple drug concentrations and contact durations. Moreover, the area under the concentration curve (AUC) and growth inhibition rate (IR) were combined, resulting in the AUC-IR curve, which was approximated to the logarithmic curve (1). We have also reported that the growth inhibition rate, calculated from the AUC-IR curve, and the actual growth inhibition rate, at an AUC of 48 μg x h/ml, are identical. Based on these results, we proposed that the in vitro antitumor effect of 5-FU depends o...

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Usefulness of collagen gel droplet embedded culture drug sensitivity testing in ovarian cancer

Cited by 10 publications

References 14 publications

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

Prediction of chemotherapeutic effect on postoperative recurrence by in vitro anticancer drug sensitivity testing in non-small cell lung cancer patients

Ribonucleotide reductase M2 is a promising molecular target for the treatment of oral squamous cell carcinoma

Evaluation of the individual 50% inhibitory area under the concentration curve of 5-fluorouracil based on the collagen gel droplet embedded culture drug sensitivity test in colorectal cancer

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