Usefulness of direct fluorescent in buffy coat in the diagnosis of Candida sepsis in neonates

Higareda-Almaraz, Martha Alicia; Loza-Barajas, H; Maldonado-González, J G; Higareda-Almaraz, Enrique; Benítez-Godínez, V; Murillo-Zamora, Efrén

doi:10.1038/jp.2016.96

Cited by 4 publications

(2 citation statements)

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“…In recent years, fluorescence staining has attracted more attention due to its potential for rapid diagnosis of fungal infections. Accumulated evidence has shown that fluorescence staining has good diagnostic efficacy in diagnosis of tinea unguium, tinea pedis, tinea capitis, corneal fungal infection and deep fungal infections (Chen et al, ; He et al, ; Higareda‐Almaraz et al, ). The mechanism involves binding of fluorescent dyes, including calcofluor white (CFW), fluorescein isothiocyanate (FITC) and acridine orange (AO), among others (Chick & Behar, ; Moussa, Tayel, & Al‐Turki, ; Sanketh, Patil, & Rao, ), to cellulose or chitin in the cell wall of the fungal through a chemical bond (Roncero & Duran, ; Roncero, Valdivieso, Ribas, & Duran, ).…”

Section: Introductionmentioning

confidence: 99%

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Fluorescence staining vs. routine KOH smear for rapid diagnosis of oral candidiasis—A diagnostic test

Zhou

Zhao

et al. 2020

Oral Diseases

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Objective A diagnostic test was designed to evaluate the accuracy and applicability of fluorescence staining with fluorescein‐labelled chitinase versus routine 10% potassium hydroxide (KOH) smear for rapid diagnosis of oral candidiasis. Methods In total, 124 subjects with suspected oral candidiasis symptoms/signs were sequentially enrolled in this study. The diagnostic efficacy indexes (sensitivity, specificity, Youden index, predictive value, likelihood ratio, diagnostic odds ratio, diagnostic accuracy and area under the curve [AUC]) were compared between 10% KOH smear and fluorescence staining. Results The sensitivity (85.48% vs. 64.52%) and specificity (91.94% vs. 72.58%) of fluorescence staining were higher than those of KOH smear. The AUC of fluorescence staining (0.887) was remarkably higher than that of 10% KOH smear (0.685), demonstrating that the diagnostic efficacy of fluorescence staining is significantly higher than that of KOH smear (p = .0005). Furthermore, fluorescence staining showed higher sensitivity than that of KOH smear (84.75% vs. 62.71%) in diagnosis of erythematous type oral candidiasis, which is the most common type and the type most challenging to differentially diagnose. Conclusion The advantages of fluorescence staining with fluorescein‐labelled chitinase in rapid diagnosis of oral candidiasis and its ease of operation suggest its potential use in clinical practice.

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Section: Introductionmentioning

confidence: 99%

“…Accumulated evidence has shown that fluorescence staining has good diagnostic efficacy in diagnosis of tinea unguium, tinea pedis, tinea capitis, corneal fungal infection and deep fungal infections (Chen et al, 2016;He et al, 2018;Higareda-Almaraz et al, 2016).…”

Section: Introductionmentioning

confidence: 99%