Background-Left ventricular dyssynchrony is often diagnosed by comparing velocity curves from Doppler tissue images (DTI) of 2 or more myocardial regions. Velocity curves are generated by placing sample volumes or "regions of interest" (ROIs) within the myocardium. ROIs need to be manually relocated to maintain a mid-myocardial location as the heart moves, but are frequently left in a stationary position. The error caused by use of a stationary ROI may affect the diagnosis of dyssynchrony, but this has not been quantified.