Usefulness of Heterologous Promoters in the<i>Pseudozyma flocculosa</i>Gene Expression System

Avis, Tyler J.; Anguenot, Raphaël; Neveu, Bertrand; Bolduc, Sébastien; Zhao, Yingyi; Cheng, Yan; Labbé, Caroline; Belzile, François; Bélanger, Richard R.

doi:10.1271/bbb.70563

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…The authors showed that a silent mutant for the biosynthesis of ergosterol of Saccharomyces cerevisiae (ERG4-) was unable to grow in the presence of medium and long chain fatty acids. Similarly, [58] reported an increased activity of cis-9-heptadecenoic acid against Phytophthora infestans, P. aphanidermatum and B. cinerea, pathogens characterized by a low or zero membrane sterol content.…”

Section: Discussionmentioning

confidence: 89%

“…The results showed that a FAm mixture at concentrations of 2 and 4% inhibited the growth of the tested phytopathogens, with an effect comparable to commercial fungicides. There are several fatty acids already known for their antifungal activity [41,57,58]. As reported by [57], some of them, such as butyric, caproic, caprylic, capric, lauric, myristic, palmitic, oleic, and linoleic acids, are able to inhibit the growth and development of phytopathogenic fungi as Alternaria solani, Colletotrichum lagenarium, Fusarium oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 98%

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Combined Biostimulant Applications of Trichoderma spp. with Fatty Acid Mixtures Improve Biocontrol Activity, Horticultural Crop Yield and Nutritional Quality

et al. 2022

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The growing demand for safer foods reflects the public perception of the adverse consequences of chemicals used in agriculture. This research aimed at developing new biological formulations based on the combination of small microbial consortia containing two Trichoderma spp., with a medium–long chain fatty acids mixture (FAm). The bioactivity of these formulations was investigated on different vegetable crops in terms of biocontrol, growth promotion, yield and quality improvements. FAm application reduced Botrytis cinerea necrosis by up to 90% compared to the infected control plants and some of the assayed Trichoderma spp. + FAm combinations contained Rhizoctonia disease, reaching more than 90% reduction of tomato and lettuce plant mortality. An increasing yield, ranging to 25 and 90%, was recorded on treated tomato, lettuce and kohlrabi compared to untreated plants. A significant enrichment in carotenoids (+60%) and glucosinolates (+39%) was measured on biotreated plants compared to controls. Untargeted LC-MS analysis revealed a higher content of tomatine and dehydro-phytosphingosine, glycoalkaloids involved in defense responses, in Trichoderma spp. + FAm combination-treated plants. The combined biostimulant applications of Trichoderma spp. with FAm were able to improve the performances of different horticultural plant species, producing a premium quality marketable vegetable with higher antioxidant content.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 98%

Combined Biostimulant Applications of Trichoderma spp. with Fatty Acid Mixtures Improve Biocontrol Activity, Horticultural Crop Yield and Nutritional Quality

et al. 2022

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“…In P. flocculosa , random mutations were accomplished by insertional mutagenesis and screened for their antagonistic properties (Cheng et al , 2003). Recently, development of the gene expression vectors and the procedure for transformation in the genus Pseudozyma were reported (Morita et al , 2007a; Avis et al , 2005, 2008; Cheng et al , 2008). Further genetic study of the genus Pseudozyma should enable us not only to understand the biological function of MELs but also to facilitate the practical use of the glycolipids on an industrial scale.…”

Section: Discussionmentioning

confidence: 99%

Identification of the gene PaEMT1 for biosynthesis of mannosylerythritol lipids in the basidiomycetous yeast Pseudozyma antarctica

Morita

Ito

Kitamoto

et al. 2010

Yeast

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The yeast Pseudozyma antarctica produces a large amount of glycolipid biosurfactants known as mannosylerythritol lipids (MELs), which show not only excellent surfaceactive properties but also versatile biochemical actions. To investigate the biosynthesis of MELs in the yeast, we recently reported expressed sequence tag (EST) analysis and estimated genes expressing under MEL production conditions. Among the genes, a contiguous sequence of 938 bp, PA 004, showed high sequence identity to the gene emt1, encoding an erythritol/mannose transferase of Ustilago maydis, which is essential for MEL biosynthesis. The predicted translation product of the extended PA 004 containing the two introns and a stop codon was aligned with Emt1 of U. maydis. The predicted amino acid sequence shared high identity (72%) with Emt1 of U. maydis, although the amino-terminal was incomplete. To identify the gene as PaEMT1 encoding an erythritol/mannose transferase of P. antarctica, the gene-disrupted strain was developed by the method for targeted gene disruption, using hygromycin B resistance as the selection marker. The obtained PaEMT1 strain failed to produce MELs, while its growth was the same as that of the parental strain. The additional mannosylerythritol into culture allowed PaEMT1 strain to form MELs regardless of the carbon source supplied, indicating a defect of the erythritol/mannose transferase activity. Furthermore, we found that MEL formation is associated with the morphology and low-temperature tolerance of the yeast.

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“…Recently, an EST (expressed sequence tag) analysis was attempted with P. antarctica grown in a medium containing soybean oil, and the genes expressed during MEL production were tentatively categorized on the basis of putative function, although the details of each gene are almost unknown [47]. The tools for genetic engineering of the yeast – the construction of the expression vector carrying efficient promoters and a simple transformation method by electroporation in Pseudozyma yeast – were also developed in the past few years [48–50].…”

Section: Biosynthetic Pathway Of Melmentioning

confidence: 99%

Production of glycolipid biosurfactants by basidiomycetous yeasts

Morita

Fukuoka

Imura

et al. 2009

Biotech and App Biochem

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BSs (biosurfactants) produced by various micro-organisms show unique properties (e.g. mild production conditions, lower toxicity, higher biodegradability and environmental compatibility) compared with chemically synthesized surfactants. The numerous advantages of BSs have prompted applications not only in the food, cosmetic and pharmaceutical industries but also in environmental protection and energy-saving technology. Among BSs, glycolipid types are the most promising, owing to their high productivity from renewable resources and versatile biochemical properties. MELs (mannosylerythritol lipids), which are glycolipid BSs abundantly produced by basidiomycetous yeasts such as strains of Pseudozyma, exhibit not only excellent interfacial properties, but also remarkable differentiation-inducing activities against human leukaemia cells. MELs also show high binding affinity towards different immunoglobulins and lectins. Recently, a cationic liposome bearing MEL has been demonstrated to increase dramatically the efficiency of gene transfection into mammalian cells. These features of BSs should broaden their application in new advanced technologies. In the present review the current status of research and development on glycolipid BSs, especially their production by Pseudozyma yeasts, is described.

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Usefulness of Heterologous Promoters in thePseudozyma flocculosaGene Expression System

Cited by 11 publications

References 24 publications

Combined Biostimulant Applications of Trichoderma spp. with Fatty Acid Mixtures Improve Biocontrol Activity, Horticultural Crop Yield and Nutritional Quality

Combined Biostimulant Applications of Trichoderma spp. with Fatty Acid Mixtures Improve Biocontrol Activity, Horticultural Crop Yield and Nutritional Quality

Identification of the gene PaEMT1 for biosynthesis of mannosylerythritol lipids in the basidiomycetous yeast Pseudozyma antarctica

Production of glycolipid biosurfactants by basidiomycetous yeasts

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