Usefulness of Sperm Quality Analyzer-v (Sqa-V) for the Assessment of Sperm Quality in Infertile Men

Akashi, Takuya; Mizuno, Ichiro; Okumura, Akiou; Fuse, Hideki

doi:10.1080/014850190959081

Cited by 16 publications

(12 citation statements)

References 20 publications

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“…Our study shows that sperm concentration results obtained by the SQA-V are in agreement with manual results, and our findings are similar to those reported earlier by Akashi et al (24) and Fuse et al (25). We found no significant linear deviation when quantifying the agreement between the two methods.…”

Section: Discussionsupporting

confidence: 94%

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Agarwal

Sharma

2007

Fertility and Sterility

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Section: Discussionsupporting

confidence: 94%

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Agarwal

Sharma

2007

Fertility and Sterility

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“…These systems are pre-calibrated vs. manual analysis with strict conformity to WHO 5th Edition and follow regular quality control protocols [9]. Several studies have demonstrated that automated sperm analyzers could provide an accurate and highly correlated alternative to manual sperm analysis [9][10][11][12][13][14][15][16][17]. Basically, there are two categories of automated sperm analyzers on the market which can be characterized by their detection technology.…”

Section: Introductionmentioning

confidence: 99%

“…The SQA-V GOLD is a fully automated system, which is based on the detection of electro-optical signals generated by moving spermatozoa and interpreted by proprietary algorithms. This signal processing for sperm motility is coupled with spectrophotometry technology to determine sperm concentration [9,12,15]. The Computer Assisted Sperm Analysis (CASA) systems are based on another principle: capturing microscopic images and image processing to detect motile and immotile spermatozoa through acquisition of rapid and successive frames [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Lammers¹,

Splingart

Barrière

et al. 2013

J Assist Reprod Genet

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Purpose Despite controversy regarding its clinical value, male fertility investigation mainly relies on semen analysis. Even though reference guidelines are available, manual sperm analysis still suffers from analytical variability, thus questioning the interest of automated sperm analysis systems. The aim of this study is to compared automated computerized semen analysis systems (SQA-V GOLD and CASA CEROS) to the conventional manual method in terms of accuracy and precision. Methods We included 250 men in this double-blind prospective study. The SQA-V GOLD (Medical Electronic Systems) and CEROS, CASA system (Hamilton Thorne) were compared to the standard manual assessment based on the WHO 5th Edition. The main outcome measures were sperm concentration, total sperm number, total motility, progressive motility, non-progressive motility, morphology, motile sperm concentration (MSC) and progressively motile sperm concentration (PMSC) with the three methods. Results Statistical analysis of the test results from the automated systems and the manual method demonstrated no significant differences for most of the semen parameters. The Spearman coefficients of rank correlation (rho) for CASA and the SQA-V GOLD automated systems vs. the manual method were: Sperm concentration (0.95 and 0.95), total sperm number (0.95 and 0.95), MSC (0.94 and 0.96) and PMSC (0.94 and 0.93) correspondingly. Concerning sperm morphology, both automated systems demonstrated high specificity (Sp) and negative predictive values (NPV), despite significantly different medians (CASA: 83.7 % for Sp and 95.2 % for NPV, SQA-V: 97.9 % for Sp and 92.5 %). The highest precision (lowest 95 % confidence interval for duplicate tests) for all semen variables was found in the SQA-V GOLD. Conclusions The advantages of using automated semen analysers are: Standardization, speed (lower turnaround time), precision, reduced potential for human error, automated data recording and less need for highly skilled professionals to run the systems. The disadvantages of using automated systems are: notably the problem with testing some atypical samples and the inability to perform an assessment of morphology abnormalities. Based on the results of this study, the SQA-V Gold demonstrated better agreement vs. the manual method. In conclusion, automated semen analyzers can be used for routine semen analysis providing rapid clinically acceptable results with higher precision, and positively impacting laboratory standardization.

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“…Moreover, different CASA systems use different mathematical algorithms to calculate various sperm quality parameters, leading to the greatest disadvantage in the clinical application of CASA, with unreliability of comparative parameters across all devices (Akashi, Mizuno, Okumura, & Fuse, 2005;Amann & Waberski, 2014;Dearing, Kilburn, & Lindsay, 2014;Kanakasabapathy et al, 2017;Lammers, Splingart, Barriere, Jean, & Freour, 2014). Moreover, different CASA systems use different mathematical algorithms to calculate various sperm quality parameters, leading to the greatest disadvantage in the clinical application of CASA, with unreliability of comparative parameters across all devices (Akashi, Mizuno, Okumura, & Fuse, 2005;Amann & Waberski, 2014;Dearing, Kilburn, & Lindsay, 2014;Kanakasabapathy et al, 2017;Lammers, Splingart, Barriere, Jean, & Freour, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Most CASA systems allow partial automation in routine sperm analysis, but they have also shown limited success due to poor accuracy at low and high sperm counts and the high cost of virtually all CASA devices. Moreover, different CASA systems use different mathematical algorithms to calculate various sperm quality parameters, leading to the greatest disadvantage in the clinical application of CASA, with unreliability of comparative parameters across all devices (Akashi, Mizuno, Okumura, & Fuse, 2005;Amann & Waberski, 2014;Dearing, Kilburn, & Lindsay, 2014;Kanakasabapathy et al, 2017;Lammers, Splingart, Barriere, Jean, & Freour, 2014). Although CASA provides quantitative data on sperm kinetics, most CASA systems still rely greatly on highly trained technicians and require an additional bulky component for data collection and computational units for data analysis.…”

mentioning

confidence: 99%

Automation of human semen analysis using a novel artificial intelligence optical microscopic technology

et al. 2019

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Current semen analysis still commonly depends on a manual microscopy method in clinical laboratories worldwide. However, some of the major disadvantages of this technique are that it is labour-intensive, subjective, laboratory-based and time-consuming. Although computer-assisted semen analysers (CASAs) have enabled partial automation of routine semen analysis, they lack wider acceptance due to their complicated operation. Therefore, the development of an accessible, rapid and standardised method for semen analysis is urgently needed. Here, we describe the development and clinical testing of a novel, automated, artificial intelligence optical microscopic (AIOM)-based technology, LensHooke™ X1 PRO (X1 PRO), designed for the quantitative measurement of sperm concentration, motility and seminal pH. We observed high degree of correlation in the results of concentration, progressive motility and progressively motile sperm concentration between the X1 PRO semen analyser and manual method using 135 clinical semen samples. In addition, the seminal pH results obtained by X1 PRO and manual methods were comparable (p = .12). In summary, our results showed that new X1 PRO semen analyser is a reliable diagnostic tool for routine semen analysis providing clinically acceptable results based on World Health Organization (WHO) 5th Edition guidelines.

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Usefulness of Sperm Quality Analyzer-v (Sqa-V) for the Assessment of Sperm Quality in Infertile Men

Cited by 16 publications

References 20 publications

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Automation of human semen analysis using a novel artificial intelligence optical microscopic technology

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