Usefulness of the 5′ region of the cDNA encoding acidic ribosomal phosphoprotein P0 conserved among rats, mice, and humans as a standard probe for gene expression analysis in different tissues and animal species

Akamine, Rie; Yamamoto, Toshiharu; Watanabe, Masahiro; Yamazaki, Naoshi; Kataoka, Masatoshi; Ishikawa, Mitsuru; Ooie, Toshihiko; Baba, Yoshinobu; Shinohara, Yasuo

doi:10.1016/j.jbbm.2006.11.008

Cited by 108 publications

(85 citation statements)

References 11 publications

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“…Q-PCR is most economical, versatile, less time consuming and highly sensitive method, which generates a T/S ratio that is proportional to a cell's average telomere length. We used single copy acidic ribosomal phosphoprotein P0 expressing 36B4 gene to compare the telomere repeat sequence, because, its cDNA nucleotide sequence has highly conserved in the 5-prime end of its open reading frame among the tissues and species making it as most preferred reference gene [30].…”

Section: Discussionmentioning

confidence: 99%

Analysis of telomere length in couples experiencing idiopathic recurrent pregnancy loss

Thilagavathi

Mishra

Kumar

et al. 2013

J Assist Reprod Genet

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Purpose Telomere length plays a significant role in various disorders; however, its role in idiopathic recurrent pregnancy loss (iRPL) is not known. The objective of this study was to assess telomere length in peripheral blood leukocytes in couples experiencing unexplained recurrent pregnancy loss (iRPL). Methods The study included 25 couples experiencing iRPL and 20 controls. The mean relative telomere length was measured by quantitative Real Time PCR (Q-PCR) based assay, which measures the average ratio of telomere repeat copy number to a single copy gene (36B4) copy number (T/S ratio) in each sample. Results The relative leukocyte mean telomere length (T/S) in both men and women from iRPL group was significantly lower (p<0.05) when compared to controls. A significant (P<0.05) negative correlation was found between age and leukocyte telomere length (T/S ratio). Among the sperm parameters seminal volume was found to be negatively (r = −0.4679) associated with the telomere T/S ratio. The DNA fragmentation index of sperm showed positive correlation (r=0.4744) with telomere length. In this preliminary study, we found that shorter telomere length in both men and women may be associated with early pregnancy loss. Conclusion In conclusion, shorter telomere length in both male and female partners appears to play a role in the idiopathic recurrent pregnancy loss. Loss of telomeric DNA due to oxidative stress needs further analysis. Analysis of telomere length in germ cells are needed to further substantiate the findings of this study.

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Section: Discussionmentioning

confidence: 99%

Analysis of telomere length in couples experiencing idiopathic recurrent pregnancy loss

Thilagavathi

Mishra

Kumar

et al. 2013

J Assist Reprod Genet

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“…All reactions were performed with Power SyBR ® Green Master Mix (Applied Biosystems) according to manufacturer's instructions. The relative amount of transcripts was calculated using comparative C T method and Acidic Ribosomal Phosphoprotein P0 mRNA was used for normalization [13].…”

Section: Atp Measurementmentioning

confidence: 99%

Mitochondrial and sarcoplasmic reticulum abnormalities in cancer cachexia: Altered energetic efficiency?

Fontes-Oliveira

Busquets

Toledo

et al. 2013

Biochimica et Biophysica Acta (BBA) - General Subjects

105

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“…Products were amplified on Applied Biosystems 7500 Real-Time PCR System (Life Technologies Co.) using iQ SYBR Green Supermix. Cycle parameters were 50 8C for 2 min and 95 8C for 10 min, followed by 44 cycles at 95 8C for 15 s, 53 8C for 30 s, and 72 8C for 45 s. The intron spanning primers used in this manuscript have been described earlier by our own group and by others (Rodgers et al 2005, Akamine et al 2007, Machado et al 2009, Paula et al 2010, 2012, Santiago et al 2011. Efficiency of each reaction was calculated using a serial dilution and varied from 94% for SIRT1 to 98% for 36B4, and products' purity was confirmed by agarose gel analysis.…”

Section: Animal Care and Treatmentsmentioning

confidence: 99%

Thyroid hormone regulation of Sirtuin 1 expression and implications to integrated responses in fasted mice

Cordeiro¹,

Souza²,

Oliveira³

et al. 2012

Journal of Endocrinology

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Sirtuin 1 (SIRT1), a NAD C -dependent deacetylase, has been connected to beneficial effects elicited by calorie restriction. Physiological adaptation to starvation requires higher activity of SIRT1 and also the suppression of thyroid hormone (TH) action to achieve energy conservation. Here, we tested the hypothesis that those two events are correlated and that TH may be a regulator of SIRT1 expression. Forty-eight-hour fasting mice exhibited reduced serum TH and increased SIRT1 protein content in liver and brown adipose tissue (BAT), and physiological thyroxine replacement prevented or attenuated the increment of SIRT1 in liver and BAT of fasted mice. Hypothyroid mice exhibited increased liver SIRT1 protein, while hyperthyroid ones showed decreased SIRT1 in liver and BAT. In the liver, decreased protein is accompanied by reduced SIRT1 activity and no alteration in its mRNA. Hyperthyroid and hypothyroid mice exhibited increases and decreases in food intake and body weight gain respectively. Food-restricted hyperthyroid animals (pair-fed to euthyroid group) exhibited liver and BAT SIRT1 protein levels intermediary between euthyroid and hyperthyroid mice fed ad libitum. Mice with TH resistance at the liver presented increased hepatic SIRT1 protein and activity, with no alteration in Sirt1 mRNA. These results suggest that TH decreases SIRT1 protein, directly and indirectly, via food ingestion control and, in the liver, this reduction involves TRb. The SIRT1 reduction induced by TH has important implication to integrated metabolic responses to fasting, as the increase in SIRT1 protein requires the fasting-associated suppression of TH serum levels.

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Usefulness of the 5′ region of the cDNA encoding acidic ribosomal phosphoprotein P0 conserved among rats, mice, and humans as a standard probe for gene expression analysis in different tissues and animal species

Cited by 108 publications

References 11 publications

Analysis of telomere length in couples experiencing idiopathic recurrent pregnancy loss

Analysis of telomere length in couples experiencing idiopathic recurrent pregnancy loss

Mitochondrial and sarcoplasmic reticulum abnormalities in cancer cachexia: Altered energetic efficiency?

Thyroid hormone regulation of Sirtuin 1 expression and implications to integrated responses in fasted mice

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