Usefulness of the Helicobacter pylori stool antigen test for detection of Helicobacter pylori infection in young Bangladeshi children

Sarker, Shafiqul Alam; Bardhan, Pradip Kumar; Rahaman, Motiur; Beglinger, Christoph; Gyr, Niklaus

doi:10.1016/s0016-5085(03)80885-5

Cited by 3 publications

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“…The specificity of the test was also very high, 92.5% (95%CI, 89–94.1%). It was generally comparable with previous HpSA reports [ 20 , 29 ], higher than some [ 25 – 27 ] and slightly lower than others [ 23 , 24 ]. This high specificity could increase the reliability of the rapid test in identifying an active infection and easy discrimination from a previous exposure.…”

Section: Discussionsupporting

confidence: 91%

“…Likewise, the negative predictive value (NPV) was also very high, 97.6% (95%CI, 93.9–99.3%). It was comparable [ 19 , 25 ], higher [ 23 , 24 ] and lower [ 26 , 27 ] when compared to previous studies. The high positive predictive value (PPV) and NPV values could show the higher accuracy of the rapid test.…”

Section: Discussionsupporting

confidence: 59%

“…The positive predictive value (PPV) of the test in the current study was also high 86.7% (95%CI, 80.5–89.6%). It was comparable [ 30 ], higher [ 25 , 26 ] and lower [ 23 , 24 ] when compared to other studies. Likewise, the negative predictive value (NPV) was also very high, 97.6% (95%CI, 93.9–99.3%).…”

Section: Discussionsupporting

confidence: 58%

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Evaluation of SD BIOLINE H. pylori Ag rapid test against double ELISA with SD H. pylori Ag ELISA and EZ-STEP H. pylori Ag ELISA tests

et al. 2018

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BackgroundHelicobacter pylori antibody titters fall very slowly even after successful treatment. Therefore, tests detecting H. pylori antibody lack specificity and sensitivity. On the other hand, H. pylori stool antigen tests are reported as an alternative assay because of their reliability and simplicity. However, the comparative performance of H. pylori stool antigen tests for detecting the presence of the bacterium in clinical specimens in the study area is not assessed. Therefore, in this study we evaluated the performance of SD BIOLINE H. pylori Ag rapid test with reference to the commercially available EZ- STEP ELISA and SD BIOLINE H. pylori Ag ELISA tests.MethodsStool samples were collected to analyse the diagnostic performance of SD BIOLINE H. pylori Ag rapid test kit using SD H. pylori Ag ELISA kit and EZ- STEP ELISA tests as a gold standard. Serum samples were also collected from each patient to test for the presence of H. pylori antibodies using dBest H. pylori Test Disk. Sensitivity, specificity, predictive values and kappa value are assessed. P values < 0.05 were taken statistically significant.ResultsStool and serum samples were collected from 201 dyspeptic patients and analysed. The sensitivity, specificity, positive and negative predictive values of the SD BIOLINE H. pylori Ag rapid test were: 95.6% (95% CI, 88.8–98.8), 92.5% (95%CI, 89–94.1%), 86.7% (95% CI, 80.5–89.6), and 97.6% (95% CI, 993.9–99.3) respectively.ConclusionThe performance of SD BIOLINE H. pylori Ag rapid test was better than the currently available antibody test in study area. Therefore, the SD BIOLINE Ag rapid stool test could replace and be used to diagnose active H. pylori infection before the commencement of therapy among dyspeptic patients.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 59%

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Evaluation of SD BIOLINE H. pylori Ag rapid test against double ELISA with SD H. pylori Ag ELISA and EZ-STEP H. pylori Ag ELISA tests

et al. 2018

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“…Eighty‐nine studies, including 10,858 patients, fulfilled the inclusion criteria and evaluated the accuracy of the stool antigen test for the diagnosis of H. pylori in untreated patients (Table 1) [14–95]. Some of the studies reviewed included, in the same protocol, patients before and after therapy, without separating them depending on the time at which the H. pylori stool antigen test was performed, thus preventing us from calculating the true diagnostic accuracy in each circumstance [96–100].…”

Section: Resultsmentioning

confidence: 99%

“…The first developed H. pylori stool antigen test, Premier Platinum HpSA™ (Meridian Diagnostics), used polyclonal antibodies to H. pylori as capture antibodies, adsorbed to microwells. The results of the test are read by spectrophotometry, although the concordance of H. pylori stool antigen test interpreted by visual reading and spectrophotometry has been very high in some studies [22,95,113]. More recently, a new stool antigen test named FemtoLab H. pylori ™ (Connex Diagnostics), a quantitative enzyme immunoassay (EIA) based on monoclonal (instead of polyclonal) antibodies, has been developed.…”

Section: Resultsmentioning

confidence: 99%

Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection: a Systematic Review

Gisbert¹,

Jm²

2004

Helicobacter

185

140

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Our aim was to review systematically the diagnostic accuracy of the Helicobacter pylori stool antigen test. Bibliographical searches were performed in several electronic databases and abstracts from congresses up to May 2003. Eighty-nine studies (10,858 patients) evaluated the stool antigen test in untreated patients. Mean sensitivity, specificity, positive predictive value and negative predictive value were 91%, 93%, 92% and 87%, respectively. Analysis of the eight studies (1399 patients) in which pretreatment evaluation of the monoclonal stool antigen test was performed showed better (p < .001) results (96%, 97%, 96% and 97%, respectively), with a clearer distinction between positive and negative results. Thirty-nine studies (3147 patients) evaluated the stool antigen test for the confirmation of H. pylori eradication 4-8 weeks after therapy, with accuracies of 86%, 92%, 76% and 93% for mean sensitivity, specificity, positive predictive value and negative predictive value, respectively. Results were similar when a gold standard based on at least two methods was used. Relatively low accuracy was reported in some posttreatment studies with the polyclonal stool antigen test. However, excellent results (p < .001) were achieved in all the six studies evaluating the monoclonal stool antigen test 4-8 weeks posttreatment. Results evaluating the stool antigen test < 4 weeks posttreatment are contradictory. Proton-pump inhibitors seem to affect the accuracy of the stool antigen test. Sensitivity and/or specificity in patients with gastrointestinal bleeding may be suboptimal. The stool antigen test performs well in children. Finally, the stool antigen test seems to be a cost-effective method.

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Non-invasive diagnostic tests forHelicobacter pyloriinfection

Best

Takwoingi

Siddique

et al. 2018

Cochrane Database of Systematic Reviews

157

130

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In people without a history of gastrectomy and those who have not recently had antibiotics or proton ,pump inhibitors, urea breath tests had high diagnostic accuracy while serology and stool antigen tests were less accurate for diagnosis of Helicobacter pylori infection.This is based on an indirect test comparison (with potential for bias due to confounding), as evidence from direct comparisons was limited or unavailable. The thresholds used for these tests were highly variable and we were unable to identify specific thresholds that might be useful in clinical practice.We need further comparative studies of high methodological quality to obtain more reliable evidence of relative accuracy between the tests. Such studies should be conducted prospectively in a representative spectrum of participants and clearly reported to ensure low risk of bias. Most importantly, studies should prespecify and clearly report thresholds used, and should avoid inappropriate exclusions.

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Usefulness of the Helicobacter pylori stool antigen test for detection of Helicobacter pylori infection in young Bangladeshi children

Cited by 3 publications

References 0 publications

Evaluation of SD BIOLINE H. pylori Ag rapid test against double ELISA with SD H. pylori Ag ELISA and EZ-STEP H. pylori Ag ELISA tests

Evaluation of SD BIOLINE H. pylori Ag rapid test against double ELISA with SD H. pylori Ag ELISA and EZ-STEP H. pylori Ag ELISA tests

Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection: a Systematic Review

Non-invasive diagnostic tests forHelicobacter pyloriinfection

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