Usefulness of the Nasal Allergen Provocation Test in the Diagnosis of Shellfish Allergy

Gelis, S; Rueda, M; Pascal, Mariona; Fernández-Caldas, E; Fernandez, Eva Abel; Araujo-Sánchez, G; Bartra, Joan; Valero, Antonio

doi:10.18176/jiaci.0736

Cited by 17 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The allergen used in the nasal provocation test was a lyophilisate of boiled shrimp, and the results were assessed with the help of acoustic rhynometry and a visual analog scale. The results of the study confirmed the usefulness of nasal allergen provocation testing in diagnosing shrimp allergy [23]. In order to diagnose food allergy in our study, apart from the established diagnostic method (DBPCFC) we used an objective method of assessing nasal mucosal reactivity and demonstrated its usefulness and safety.…”

Section: Discussionsupporting

confidence: 75%

Nasal mucosal reactivity assessment via a double-blind placebo-controlled food challenge with cow’s milk allergens

Krzych‐Fałta

Wojas

Samel-Kowalik

et al. 2022

Allergy Asthma Clin Immunol

View full text Add to dashboard Cite

Background Allergies, including food allergies, are a considerable clinical and public-health problem. The introduced preventive measures and differential diagnostics, including oral food challenges, are the gold standard for determining further treatment planning. Case presentation We present a case of an 18-year-old girl with a cow’s milk allergy who underwent an oral food challenge (double blind oral food challenge). Such a challenge may be confounded by inducing a response from other systems and organs, which provides theoretical grounds for the use of other methods of assessing the body’s response to food allergens (response demonstrated by the upper respiratory tract). Based on this idea, in order to assess the degree of mucosal response, we used optical rhinometry as an objective method for nasal patency evaluation, as well as identification of tryptase level in nasal lavage fluid and exfoliative cytology of nasal mucosa. The results of these tests confirmed positive reaction of the nasal mucosa in the course of the oral allergen challenge. Conclusions The observed increase in the nasal mucosal reactivity that accompanies oral food challenges may suggest a potential for using food allergens in nasal allergen provocation testing in order to diagnose food allergies.

show abstract

Section: Discussionsupporting

confidence: 75%

Nasal mucosal reactivity assessment via a double-blind placebo-controlled food challenge with cow’s milk allergens

Krzych‐Fałta

Wojas

Samel-Kowalik

et al. 2022

Allergy Asthma Clin Immunol

View full text Add to dashboard Cite

show abstract

“…Boiled shrimp lyophilisate was used for the nasal test and the results were assessed on the basis of acoustic rhinometry and visual analog scale. The results confirmed the usefulness of the nasal allergen provocation test in the diagnostics of shellfish food allergies [19].…”

Section: A B Csupporting

confidence: 73%

Reactivity of nasal cavity mucosa in the nasal cow’s milk allergen provocation test

Krzych‐Fałta¹,

Wojas²,

Namysłowski³

et al. 2023

pdia

View full text Add to dashboard Cite

Introduction:The nasal allergen provocation test plays an important role in differential diagnostics of rhinitis. Due to its informative potential, the test is also becoming increasingly used in other areas of diagnostics, including the diagnostics of food allergies. Aim: To assess the reactivity of nasal mucosa to the cow's milk protein allergens (as being widely used in powdered form in the food industry). Material and methods:The study material consisted of a group of 31 healthy subjects not sensitized to environmental allergens including cow's milk protein allergens. The study method involved an incremental nasal provocation test with cow's milk protein evaluated using the visual analog scale and acoustic rhinometry. Results: A total of 29 out of 31 volunteers presented with a significant decrease in nasal patency (control solution: 1.112 ±0.161 vs. local allergen application 1.005 ±0.157; p < 0.004) as measured by acoustic rhinometry following the allergen dose of 12.5 µg. Slight changes in complaints were observed using the visual analog scale. Exposure to the widespread food allergens (including powdered cow's milk allergens) presents a potential risk of positive response in non-sensitized individuals. Conclusions: Further studies on dose standardization are necessary in the study area.

show abstract

“…Our recent report clearly reveals that diagnosis based on clinical history and extract‐based serum‐specific IgE (sIgE) test was poor in differentiating shrimp allergy and tolerance in a cohort of double‐blind placebo‐controlled food challenge (DBPCFC) proven subjects. 6 While nasal allergen provocation test was also shown as a valuable test differentiating sensitized symptomatic shrimp allergic patients from sensitized tolerant subjects, 7 oral food challenges (OFCs) remain the gold standard in diagnosing shrimp allergy.…”

Section: Introductionmentioning

confidence: 99%

Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy

Wai

Leung

et al. 2022

Allergy

View full text Add to dashboard Cite

Background Clinical management of shrimp allergy is hampered by the lack of accurate tests. Molecular diagnosis has been shown to more accurately reflect the clinical reactivity but the full spectrum of shrimp allergens and their clinical relevance are yet to be established. We therefore sought to comprehend the allergen repertoire of shrimp, investigate and compare the sensitization pattern and diagnostic value of the allergens in allergic subjects of two distinct populations. Methods Sera were collected from 85 subjects with challenge‐proven or doctor‐diagnosed shrimp allergy in Hong Kong and Thailand. The IgE‐binding proteins of Penaeus monodon were probed by Western blotting and identified by mass spectrometry. Recombinant shrimp allergens were synthesized and analyzed for IgE sensitization by ELISA. Results Ten IgE‐binding proteins were identified, and a comprehensive panel of 11 recombinant shrimp allergens was generated. The major shrimp allergens among Hong Kong subjects were troponin C (Pen m 6) and glycogen phosphorylase (Pen m 14, 47.1%), tropomyosin (Pen m 1, 41.2%) and sarcoplasmic‐calcium binding protein (Pen m 4, 35.3%), while those among Thai subjects were Pen m 1 (68.8%), Pen m 6 (50.0%) and fatty acid‐binding protein (Pen m 13, 37.5%). Component‐based tests yielded significantly higher area under curve values (0.77–0.96) than shrimp extract‐IgE test (0.70–0.75). Yet the best component test differed between populations; Pen m 1‐IgE test added diagnostic value only in the Thai cohort, whereas sensitizations to other components were better predictors of shrimp allergy in Hong Kong patients. Conclusion Pen m 14 was identified as a novel shrimp allergen predictive of challenge outcome. Molecular diagnosis better predicts shrimp allergy than conventional tests, but the relevant component is population dependent.

show abstract

Usefulness of the Nasal Allergen Provocation Test in the Diagnosis of Shellfish Allergy

Cited by 17 publications

References 0 publications

Nasal mucosal reactivity assessment via a double-blind placebo-controlled food challenge with cow’s milk allergens

Nasal mucosal reactivity assessment via a double-blind placebo-controlled food challenge with cow’s milk allergens

Reactivity of nasal cavity mucosa in the nasal cow’s milk allergen provocation test

Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy

Contact Info

Product

Resources

About