Usefulness of the organ culture system in the in vitro diagnosis of Celiac Disease: A multicenter study

Picarelli, Antonio; Tola, Marco Di; Sabbatella, L.; Anania, M.C.; Calabrò, Antonino; Renzi, Daniela; Bai, Jing; Sugai, Emilia; Carroccio, Antonio; Prima, Lidia Di; Bardella, M.T.; Barisani, Donatella; Ribes-Koninckx, Carmen; Aliaga, Ester Donat; Gasparin, Maurizio; Bravi, E.; Casale, C.; Vetrano, Stefania; Lettieri, F.; Maffia, C.; Picchi, C.; Turriziani, S.; Calella, Francesca; Grappone, Cecilia; Paoli, B.; Niveloni, Sonia I.; Mauriño, Eduardo; Vitali, Roberta; Elli, Luca; Miquel, Begoña Polo

doi:10.1016/s1590-8658(06)80212-4

Cited by 2 publications

(4 citation statements)

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“…Celiac disease was diagnosed in accordance with the procedure adopted worldwide [22], which includes the serological screening of at-risk populations and duodenal biopsy histology. As previously suggested [23][24][25], the organ culture system was used as an additional tool to support the diagnosis. All the participants in the study were screened for CD by using serum IgA/IgG EMA and/or antitissue transglutaminase (anti-tTG) antibody tests.…”

Section: Diagnosis Of Celiac Diseasementioning

confidence: 99%

Type 1 diabetes mellitus and celiac disease: endothelial dysfunction

et al. 2011

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Many reports indicate a hypercoagulative state in diabetes mellitus as result of endothelial damage. Experimental evidence suggests that a metabolic derangement triggers a cascade of biochemical events that lead to vascular dysfunction. The net effect is to convert the endothelium from thromboresistant to thrombogenic surface. In literature, a strong association between type 1 diabetes mellitus (DM1) and celiac disease (CD) has been reported. We do not have information about the hemostatic system in these associated conditions. Our study aims at evaluating whether the presence of CD in a group of DM1 patients is associated with a different expression of some hemostatic factors and with a different manifestation and/or progression of microvascular complications of DM1 in comparison with patients with only diabetes. Ninety-four adult DM1 patients were enrolled in the study and subsequently screened for CD. Anti-endomysial antibodies (EMA) were positive in 13 of 94 DM1 patients (13.8%). CD diagnosis was confirmed by histology and organ culture. The mean age and duration of DM1 of patients also affected by CD were similar to those of only diabetic patients, but the metabolic control and the hemocoagulative parameters were significantly different between the two groups: DM1 patients also affected by CD presented significantly lower concentrations of glycosylated hemoglobin (HbA1c) (P < 0.05), cholesterol (P < 0.001), triglycerides (P < 0.001), factor VII antigen (FVII:ag) (P < 0.005), factor VII coagulant activity (FVII:c) (P < 0.05), and prothrombin degradation fragments (F1+2) (P < 0.001), as well as higher values of activated C protein (APC) (<0.001). No retinal abnormalities and no signs of renal damage were observed in DM1 patients also affected by CD. Our results suggest a potential protective role of CD in the prothrombotic state of DM1.

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Section: Diagnosis Of Celiac Diseasementioning

confidence: 99%

Type 1 diabetes mellitus and celiac disease: endothelial dysfunction

et al. 2011

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“…The presence of EMA and anti‐tTG measured by ELISA in duodenal culture media of all CD patients but not of disease controls firstly confirm the reliability of the organ culture system in identifying CD . Using ICA, anti‐tTG antibodies are detectable in duodenal culture media of most CD patients but not of disease controls, highlighting the reliability and usefulness of this method in identifying the organ cultures as positive, while negative results need to be confirmed by the standard ELISA technique.…”

Section: Discussionmentioning

confidence: 70%

“…As a consequence, some in vitro methods have been proposed as supporting tools to diagnose CD . Among these, the organ culture system has been proposed as a supporting tool for duodenal histology, especially in cases with patchy atrophy or potential CD where, using only the conventional histology, the risk of missing the diagnosis is very high . This system is further able to evaluate the production/release of antibodies pathognomonic of CD directly from duodenal mucosa, adding advantages in terms of both sensitivity and specificity with respect to detection of the same antibodies in serum.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that EMA and anti‐tTG can also be found in culture supernatants of duodenal biopsies from untreated CD patients . This organ culture system has been proposed in the diagnostic procedure of CD, especially for cases with patchy atrophy or potential CD where, using only the conventional histology, the risk of missing the diagnosis is very high . However, some conflicting opinions and a number of technical difficulties have limited its widespread use in the diagnosis of CD …”

Section: Introductionmentioning

confidence: 99%

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