Usefulness of Two-Step Algorithm with Earlier Growth Detection in Anaerobic Bottle and Time to Positivity to Predict <i>Candida glabrata</i> Fungemia

Byun, Jung Hyun; Lee, Dong-Hyun; Kim, Sun‐Joo

doi:10.5145/acm.2018.21.2.23

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…The TTD of C. glabrata in the FN Plus bottle was shorter than that of the Old and New FA Plus bottles (Old FA Plus 52.47 hours and New FA Plus 19.92 hours versus FN Plus 16.4 hours; P < 0.001). The increased proliferation of C. glabrata in the anaerobic bottle was consistent with several previous studies [ 11 – 13 ]. The yeast cell count was significantly higher in the FN Plus bottle compared with the Old FA Plus ( P <0.001), but yeast cell count difference was not statistically significant with New FA Plus ( P = 0.1581).…”

Section: Resultssupporting

confidence: 92%

Comparison of new and old BacT/ALERT aerobic bottles for detection of Candida species

Choe,

Lim,

Lee

2023

PLoS ONE

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Purpose A new version of aerobic blood culture media has been developed for the BacT/ALERT (bioMérieux) blood culture system. We evaluated the time to detection and yeast cell counts in positive blood cultures for each Candida spp. according to changes in media. Methods Isolates from defibrinated horse blood were inoculated into three types of bottles: the old version of aerobic bottle, new version of aerobic bottle, and anaerobic bottle. All bottles were incubated in the BacT/ALERT Virtuo blood culture system. The time to detection was monitored for each bottle, and yeast cell counts were performed immediately after testing positive, determined via the plate count method. Clinical retrospective data of the candidemia samples before and after aerobic bottle change also were analyzed. Results The median time to detection was 52.47 hours in the old aerobic bottles versus 19.92 hours in the new aerobic bottles (P < 0.001) for Candida glabrata, and standard and clinical strains showed similar results. C. albicans (27.6 to 24.95 hours) and C. guilliermondii (28.92 to 26.9 hours) had shorter time to detection. However, C. auris (25.43 to 28.25 hours) had a longer time to detection in the new aerobic bottle. The retrospective clinical analysis showed a significant decrease in time to detection (45.0 to 19.4 hours) for C. glabrata, which is consistent with our simulated study result for C. glabrata. As a result of analysis including all blood specimens, C. tropicalis showed a significant delay in time to detection in new aerobic bottles. In an analysis limited to peripheral blood specimens, the time to detection of C. parapsilosis was longer in new aerobic bottles than in old aerobic bottles. Conclusion Most Candida species did not show remarkable TTD differences, but TTD of C. glabrata was markedly reduced in the New FA Plus bottle. The reduction of time to detection enables faster detection and therapeutic approach for C. glabrata infections.

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Section: Resultssupporting

confidence: 92%

Comparison of new and old BacT/ALERT aerobic bottles for detection of Candida species

Choe,

Lim,

Lee

2023

PLoS ONE

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“…The TTD values were compared among the 14 isolates performed in triplicate for each bottle type (Table 2). The mean TTD of C. glabrata was 51. previous studies [11][12][13]. The yeast cell count was significantly higher in the FN Plus bottle compared with the old and new aerobic bottles (P = 0.002).…”

Section: Simulated Study Datamentioning

confidence: 59%

Comparison of New and Old BacT/Alert Aerobic Bottles for Detection ofCandidaspp.

Choe

Lim

Lee

2023

Preprint

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Purpose A new version of aerobic blood culture media has been developed for the BacT/Alert (bioMerieux) blood culture system. We evaluated the TTD and yeast cell counts in positive blood cultures for each Candida spp. according to changes in media. Methods Isolates from defibrinated horse blood were inoculated into three types of bottles: the old version of FA Plus (Old FA Plus), New FA Plus, and FN Plus. All bottles were incubated in the BacT/Alert Virtuo blood culture system. The TTD was monitored for each bottle, and yeast cell counts were performed immediately after testing positive, determined via the plate count method. Clinical retrospective data of the candidemia samples before and after aerobic bottle change also were analyzed. Results The mean TTD was 51.2 hours in the Old FA Plus bottles versus 19.5 hours in the New FA Plus bottles (P < 0.001) for Candida glabrata, and standard and clinical strains showed similar results. C. albicans (27.6 to 25.1 hours) and C. guilliermondii (28.8 to 27.3 hours) had shorter TTDs. However, C. auris (25.6 to 28.1 hours) had a longer TTD in the New FA Plus bottle. The retrospective clinical analysis showed a significant decrease in TTD (45.0 to 19.4 hours) for C. glabrata, which is consistent with our simulated study. Conclusion This study shows that the TTD of C. glabrata was markedly reduced in the New FA Plus bottle due to the optimized growth performance. The reduction of TTD enables faster detection and therapeutic approach for C. glabrata infections.

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Usefulness of Two-Step Algorithm with Earlier Growth Detection in Anaerobic Bottle and Time to Positivity to Predict Candida glabrata Fungemia

Cited by 2 publications

References 16 publications

Comparison of new and old BacT/ALERT aerobic bottles for detection of Candida species

Comparison of new and old BacT/ALERT aerobic bottles for detection of Candida species

Comparison of New and Old BacT/Alert Aerobic Bottles for Detection ofCandidaspp.

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