Usefulness of UF-5000 automatic screening system in UTI diagnosis

Toledo, Héctor; Punzón, Sara G.; Martín-Gutiérrez, Guillermo; Pérez, Juan A.; Lepe, José A.

doi:10.1007/s42770-023-01052-9

Cited by 2 publications

(5 citation statements)

References 22 publications

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“…In this study, positive urine culture results were 13.0% (513/3956). This result is lower than that reported in previous studies involving adults, researchers report 20% -40% of positive cultures in most studies [ 6 , 12 , 13 , 16 ], but 10.4% in one study of children [ 17 ], aligning with our laboratory results. The reasons for this variance may be due to the non-specific clinical manifestations of children, making the diagnosis of UTIs in the pediatric population challenging.…”

Section: Discussionsupporting

confidence: 61%

“…For BACT and WBC counts in urinary samples, a recent study showed that UF-5000 exhibited acceptable SEs (96.1% and 91%) and lower SPs (32.2% and 28%) at 100 bacteria/μL and 21 WBCs/μL, respectively [ 16 ]. In another study, a BACT count at a cut-off of 135/μL, the SE and SP were 92.1% and 85.4%.…”

Section: Discussionmentioning

confidence: 99%

“…However, De Rosa et al reached 55% [ 6 ]. In addition, Toledo et al reduced unnecessary cultivation by 40% at a sensitivity of 95% [ 16 ], but these data are all from studies on adults. Our laboratory achieved a 57.8% reduction in unnecessary cultivation at a sensitivity of 95%.…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon may be related to the tendency of GP cocci to form bacterial aggregates, which interfere with cytometer readings. Toledo et al [ 16 ] and Manoni et al [ 24 ] reported that GP cocci were obtained from over half of the urine samples that were false-negative. This finding is consistent with our conclusions.…”

Section: Discussionmentioning

confidence: 99%

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Enhancing clinical decision-making: Sysmex UF-5000 as a screening tool for bacterial urinary tract infection in children

Liu,

Ban,

Wang

et al. 2024

PLoS ONE

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Background A rapid screening test for urinary tract infections (UTIs) in children is needed to avoid unnecessary cultures and provide prompt reports to make appropriate clinical decisions. We have evaluated for the first time the performance of the Sysmex UF-5000 flow cytometer as a screening tool for UTIs in children. Methods This study included 4445 pediatric patients, with urinary sediment and urine culture data collected from January 2020 to September 2023. The Sysmex UF-5000 analyzer was utilized to measure urine white blood cell (WBC) and bacteria (BACT), with the findings being compared to the culture results. Results At ≥ 104 colony-forming unit (CFU)/mL, 513 samples were culture-positive (400 samples presented 104–105 CFU/mL, and 113 demonstrated ≥ 105 CFU/mL bacterial growth). Optimal indicators for positive cultures were BACT counts of 92.2/μL (AUC: 0.944) and WBC counts of 40.8/μL (AUC:0.863). False negative rate were 0.9% when using a 7.8 bacteria/μL cut-off and avoiding unnecessary cultures in 28.1%. The UF-5000 has a higher consistency rate for Gram-negative (GN) bacteria (90.3%) than Gram-positive (GP) bacteria (86.8%). For samples with 105 CFU/mL, UF-5000’s Bacteria -Information flags showed superior concordance for samples with 104−105 CFU/mL bacteria. Conclusions Screening pediatric urine cultures with the UF-5000 showed potential application value in identifying negative cultures and significant bacterial growth, although performance may vary depending on the study population. Furthermore, detecting Gram typing aids in guiding early clinical empirical medication, particularly for UTIs caused by GN bacteria.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Enhancing clinical decision-making: Sysmex UF-5000 as a screening tool for bacterial urinary tract infection in children

Liu,

Ban,

Wang

et al. 2024

PLoS ONE

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“…Consequently, automated flow cytometry-based analyzers have been introduced to allow for a more robust screening of urine samples for WBC counts and bacterial load differentiation [39,40]. Although negative results from these analyzers have yielded excellent negative predictive value and could streamline the workflow, the specificities and positive predictive values for UTI diagnosis have been reported to be suboptimal, and their ability to discriminate between Gram-negative and Gram-positive UTIs is low [41][42][43]. In our locality, a recent study found that the concordance rate between a flow cytometry analyzer and culture results for predicting Gram-positive or Gram-negative UTIs was 38% [41].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples

Tang,

Lee,

et al. 2023

Antibiotics

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Infections caused by extended-spectrum β-lactamase-producing Enterobacterales have increased rapidly and are mainly attributed to the production of CTX-M enzymes. This study evaluated the NG-Test® CTX-M MULTI lateral flow assay (CTX-M LFA) and the Rapid ESBL NP® test (ESBL NP test) for rapid detection of CTX-M-producing Enterobacterales directly in midstream urine (MSU) samples. Testing was performed on 277 clinical MSU samples in a hospital microbiology laboratory from November 2022 to January 2023; 60 of these samples (30 positive for ESBL producers and 30 positive for non-ESBL producers) were tested retrospectively after the identification and susceptibility results were obtained, and 217 samples were tested prospectively immediately after a Gram stain showing the presence of Gram-negative bacilli. The results were compared against phenotypic detection of ESBL and molecular testing as the reference methods. Overall, 67 of the 277 samples were culture-positive for ESBL-producing Enterobacterales. PCR for the blaCTX-M gene was positive for all ESBL-producing Enterobacterales isolates. All CTX-M LFA results were interpretable, while three of the ESBL NP test results were noninterpretable. The sensitivity of the CTX-M LFA (100%, 95% CI 94.6–100%) was higher than that of the ESBL NP test (86.6%, 95% CI 76.0–93.7%). Both tests had high specificities (CTX-M LFA, 99.1%, 95% CI 96.6–99.9% and ESBL NP test, 100%, 95% CI 98.2–100%). In conclusion, both the CTX-M LFA and the ESBL NP test can deliver rapid results that could improve antimicrobial stewardship for urinary tract infections.

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Usefulness of UF-5000 automatic screening system in UTI diagnosis

Cited by 2 publications

References 22 publications

Enhancing clinical decision-making: Sysmex UF-5000 as a screening tool for bacterial urinary tract infection in children

Enhancing clinical decision-making: Sysmex UF-5000 as a screening tool for bacterial urinary tract infection in children

Evaluation of Two Tests for the Rapid Detection of CTX-M Producers Directly in Urine Samples

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