User-friendly analysis of droplet array images

A Guide to Biodetection in Droplets

“…An overall ideal solution would be to omit fluorescence by use of label-free optical detection as exhibited by several studies covered in this review. ,− Nevertheless, the main drawback with label-free detection in droplet emulsions that researchers should be aware of is the need to often apply complicated and custom-made scripts for data analysis. − Analysis is also complicated in experiments where polydisperse droplets are generated via, for example, simple and quick vortexing. ,, Yet, when assessing the current state and future direction of optical biodetection with droplet emulsion methods, as well as ways to improve accessibility to the general scientific public, polydisperse generation could be one part of the solution. In particular, this is true because analysis processing via deep learning algorithms is quickly advancing , and the development of user-friendly and freely accessible pipelines from droplet image analysis is robust. − Making droplet emulsion generation quick and simple, moreover, especially correlates with the current trend seen in the field of nucleic acid detection. The focus is shifting toward not only high-resolution single-cell analysis ,, but also rapid and easy to use diagnostic and monitoring tools. ,,,− Nevertheless, when addressing detection of extremely small molecules in complex matrices (e.g., neurotransmitters and electrolytes), options are restrictive due to current scientific technological limitations.…”

Droplet-Based Technology for Studying the Phenotypic Effect of Microplastics on Antimicrobial Resistance

“…In parallel, we encapsulated single cells of E. coli into droplets with different concentrations of cefotaxime and with or without PS. After overnight incubation at 37 • C, we imaged droplets as a monolayer via confocal microscopy and analyzed droplets via Software Ilastik [8], CellProfiler TM [9] and EasyFlow [10]. Our results show that E. coli's minimal inhibitory concentration (MIC) shifts slightly towards a higher cefotaxime concentration when PS is present in droplets.…”

Critical Review of Techniques for Food Emulsion Characterization

“…Dynamic light scattering, small-angle X-ray scattering, ultrasonic spectrometry, electrical pulse counting, field-flow fractionation, and capillary hydrodynamic fractionation [8] are key tools used for droplet size analysis. The understanding of emulsion behaviors can be further enhanced by microscopic analysis, which provides fundamental information about the morphology of the droplets, their concentration, and distribution [9][10][11]. Various techniques, such as optical microscopy, atomic force microscopy, transmission, or scanning electron microscopy, enable researchers to assess the structural properties of emulsions [12].…”