Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites

Al-Badrani, B. A.; Hammed, Sara Mohammed

doi:10.21608/ejvs.2021.96527.1298

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Hansen et al (36) described acridine orange staining as a fluorochrome stain for the detection of deoxyribonucleic acid (DNA) in bovine babesiosis (37,38). Ravindran et al (39) described the acridine orange staining technique as an accurate, simple, and rapid screening of many blood samples. It was also reported that time for identification of first parasite was only 1/3 by acridine orange staining as compared to giemsa-stained smears (40,41).…”

Section: Discussionmentioning

confidence: 99%

A comparison between different laboratory methods and stains for detection microfilaremic dogs

Al-Malachi¹,

Al-Farwachi²

2023

IJVS

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For microscopical and statistical evaluation of different laboratory methods and stains for detecting circulating microfilariae of Dirofilaria immitis in ten naturally infected local stray dog's positive to canine heartworm Ag ELISA kit. All blood samples were examined by three methods modified Knott's technique, serum concentration test, and Türk's solution concentration test and three laboratory stains methylene blue, giemsa, and acridine orange stains were used to dye the precipitate of the modified Knott's technique. Results showed that the slides prepared from the serum were easy to examine (there are no erythrocytes) compared with the slides prepared from the precipitate of the modified Knott's technique. The slides prepared using the Türk's solution were more transparent and freer of cell debris. The length and width of the microfilariae detected by the three methods did not change significantly. Our conclusion from this study is that Türk's solution concentration test and the serum concentration test can be used in place of the modified Knott's technique to detect microfilaremic dogs. Acridine orange staining can also substitute for methylene blue and giemsa stains for faster results.

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Section: Discussionmentioning

confidence: 99%

A comparison between different laboratory methods and stains for detection microfilaremic dogs

Al-Malachi¹,

Al-Farwachi²

2023

IJVS

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Cross-sectional study on prevalence of theileriosis in Kendrapada sheep, Odisha

Mohanty,

Acharya,

Mishra

et al. 2024

Preprint

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Ovine theileriosis represents a significant threat to both animal welfare and global agricultural productivity, particularly impacting economically disadvantaged farmers. Vector-borne haemoparasitic diseases like theileriosis have a profound effect on the health of livestock, leading to increased illness and mortality, especially in the coastal regions of Odisha. This research aimed to investigate and comprehend the prevalence, clinical manifestations, hematological and biochemical parameters, as well as molecular aspects of theileriosis in sheep from Kendrapada. Among 148 blood samples collected from Kendrapada sheep across four blocks, 13% tested positive for theileriosis, indicating a notable prevalence. The prevalence rate was highest among sheep aged 1–4 years (19%), followed by those younger than 1 year (17.9%) and older than 4 years (13.3%). Female sheep exhibited a higher prevalence (19%) compared to males (16%). Block-wise prevalence was highest in Aul (27.9%), followed by Marshaghai (19.4%), Kendrapada (16.1%), and Derabish (5.88%). Hematological analysis revealed deviations in parameters such as hemoglobin, total erythrocyte count, total leukocyte count, and mean corpuscular volume, while packed cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and differential leukocyte count remained within normal ranges. Significant differences (P < 0.05) were observed in various parameters between Theileria-positive and negative sheep. However, there were no substantial variations noted among different age groups and genders of Theileria-positive sheep. PCR analysis identified an overall incidence of theileriosis at 17.57% in the study population, with sequences closely resembling Theileria luwenshuni isolates. This study confirms the high variability of the 18S small subunit rRNA gene among Theileria spp.

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Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites

Cited by 2 publications

References 26 publications

A comparison between different laboratory methods and stains for detection microfilaremic dogs

A comparison between different laboratory methods and stains for detection microfilaremic dogs

Cross-sectional study on prevalence of theileriosis in Kendrapada sheep, Odisha

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