2018
DOI: 10.20546/ijcmas.2018.705.090
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Uses of Transmission Electron Microscope in Microscopy and its Advantages and Disadvantages

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Cited by 13 publications
(3 citation statements)
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“…C-Dots were observed on the lumps after being characterized using a high-resolution transmission electron microscope (HRTEM). TEM has a superior resolution of 0.1 nm -0.2 nm, which is higher than that of SEM (1 nm -3 nm) [22]. The SEM images that were processed using Image-J digital data processing software with Analyze Particles feature are shown in Figure 11.…”
Section: Resultsmentioning
confidence: 99%
“…C-Dots were observed on the lumps after being characterized using a high-resolution transmission electron microscope (HRTEM). TEM has a superior resolution of 0.1 nm -0.2 nm, which is higher than that of SEM (1 nm -3 nm) [22]. The SEM images that were processed using Image-J digital data processing software with Analyze Particles feature are shown in Figure 11.…”
Section: Resultsmentioning
confidence: 99%
“…The sample preparation can lead to artifacts and the double membrane is harder to detect in formalin-fixed samples. Therefore, the operator needs to be well-trained in using TEM and also in the analysis and interpretation of the images [145]. A recent study has reported that snap-frozen cryo-fixation of tissue pieces followed by 1.5% paraformaldehyde post-fixation can help to reveal autophagosome structures [146].…”
Section: Current Autophagy Markers and Their Limitationsmentioning
confidence: 99%
“…For example, the images (micrographs) generated by transmission electron microscopy, a widely used microanalysis technique, currently rely on manual labeling by experienced researchers [16], but manually labeling large numbers of micrographs is infeasible, and the need for skilled professionals continues to grow [17,18]. Additionally, obtaining the correct configuration of transmission electron microscopy, which is related to quality parameters and research subjects, is still a complex process.…”
Section: Introductionmentioning
confidence: 99%