2024
DOI: 10.1021/acs.accounts.3c00682
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Using 2D-IR Spectroscopy to Measure the Structure, Dynamics, and Intermolecular Interactions of Proteins in H2O

Neil T. Hunt

Abstract: Metrics & MoreArticle Recommendations CONSPECTUS: Infrared (IR) spectroscopy probes molecular structure at the level of the chemical bond or functional group. In the case of proteins, the most informative band in the IR spectrum is the amide I band, which arises predominantly from the C�O stretching vibration of the peptide link. The folding of proteins into secondary and tertiary structures leads to vibrational coupling between peptide units, generating specific amide I spectral signatures that provide a fing… Show more

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Cited by 10 publications
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“…The major amide I band of the PTTs was observed at 1620 cm –1 , indicating that the β-sheet is the major secondary structure of PTTs (Figure b). D 2 O was used as the solvent because the vibrational frequency of the H 2 O bending mode overlaps in this region, whereas that of D 2 O is observed at 1100–1300, and does not interfere with the interpretation of the amide I band. , As the temperature increased from 20 to 50 °C, the ratio of absorbance at 1620 to 1454 cm –1 increased, suggesting that the β-sheet content partially increased during the sol-to-gel transition (Figure b inset) . In addition, 1 H NMR spectra were also monitored as the temperature increased from 20 to 50 °C.…”
Section: Resultsmentioning
confidence: 99%
“…The major amide I band of the PTTs was observed at 1620 cm –1 , indicating that the β-sheet is the major secondary structure of PTTs (Figure b). D 2 O was used as the solvent because the vibrational frequency of the H 2 O bending mode overlaps in this region, whereas that of D 2 O is observed at 1100–1300, and does not interfere with the interpretation of the amide I band. , As the temperature increased from 20 to 50 °C, the ratio of absorbance at 1620 to 1454 cm –1 increased, suggesting that the β-sheet content partially increased during the sol-to-gel transition (Figure b inset) . In addition, 1 H NMR spectra were also monitored as the temperature increased from 20 to 50 °C.…”
Section: Resultsmentioning
confidence: 99%