Using a combined oxygen‐supply and substrate‐feeding strategy to improve 2,3‐butanediol production by metabolically engineered <i>Klebsiella oxytoca</i> KMS005

Chan, Sitha; Kanchanatawee, Sunthorn; Jantama, Sirima Suvarnakuta; Jantama, Kaemwich; Joannis-Cassan, Claire; Taillandier, Patricia

doi:10.1002/jctb.5409

Cited by 5 publications

(10 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…47 Various metabolic products (e.g., ethanol, acetate, lactate, succinate, formate) could be produced depending on the microorganism and culture conditions. 50 Bacterial growth, BDO, acetoin, ethanol, and organic acid synthesis are influenced by oxygen availability. As observed in this study, the production of byproducts varied with glycerol purity and k L a value (Supporting Information, Figure S4).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Bioprocess Development for 2,3-Butanediol Production from Crude Glycerol and Conceptual Process Design for Aqueous Conversion into Methyl Ethyl Ketone

Maina

Dheskali²,

Papapostolou

et al. 2021

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

The transition to the circular economy era necessitates the sustainable production of chemicals using crude renewable resources via integrated biological and chemical conversion routes. Within this concept, this study combined experimental fermentation development with conceptual process design and technoeconomic evaluation to demonstrate the feasibility of bio-based methyl ethyl ketone (MEK) production from crude glycerol derived from industrial biodiesel production using soybean oil alone or mixed with tallow fat. 2,3-Butanediol (BDO) production via fermentation of crude glycerol was initially developed using a Klebsiella michiganensis strain leading to 76.1 g/L BDO concentration with a productivity of 1.38 g/(L h) at a volumetric mass transfer coefficient of 77 h–1. Direct aqueous BDO conversion into MEK followed by purification via pressure swing distillation was subsequently evaluated via conceptual process design. The bio-based MEK minimum selling price (MSP) is close to the 11 year average market price of petroleum-derived MEK ($1.8/kg) when the crude glycerol cost is up to $0.1/kg at annual MEK production capacities higher than ca. 20 000 t. Monte Carlo simulations showed ca. 30% probability of obtaining an MSP lower than $1.8/kg at a crude glycerol cost of $0.1/kg. This study demonstrated the feasibility of cost-competitive bio-based MEK production as an industrial solvent and an intermediate chemical.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

Bioprocess Development for 2,3-Butanediol Production from Crude Glycerol and Conceptual Process Design for Aqueous Conversion into Methyl Ethyl Ketone

Maina

Dheskali²,

Papapostolou

et al. 2021

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…To develop an efficient process for 2,3‐BD production from cassava starch, a fed‐batch strategy was carried out under constant feeding rate. In a previous study, fed‐batch fermentation by the use of an initial sugar concentration of 90 g L −1 derived from maltodextrin enhanced 2,3‐BD production with less residual sugar concentration 16 . Thus the initial cassava starch concentration of 90 g L −1 was used to produce 2,3‐BD under fed‐batch mode.…”

Section: Resultsmentioning

confidence: 99%

“…The percentage of carbon recovery was calculated by dividing total carbon moles of fermentative products produced (2,3‐BD, acetate, succinate, ethanol, CO 2 and biomass) by total carbon moles of equivalent glucose (from cassava starch) utilized during fermentation. The formula was represented in Chan et al 16 …”

Section: Methodsmentioning

confidence: 99%

“…Several microbial species, including Paenibacillus polymyxa , 6 Serratia marcescens , 7 Bacillus amyloliquifaciens , 8 Enterobacter aerogenes , 9 Klebsiella pneumonia and K. oxytoca 10 , can convert sugars to 2,3‐BD. Most studies of 2,3‐BD production by these strains involved investigation using pure or easily fermentable substrates such as glycerol, sucrose, glucose, xylose or maltodextrin 7,11–16 . However, these processes may not be economically feasible for industrial applications due to the high costs of these pure sugars.…”

Section: Introductionmentioning

confidence: 99%

“…Chan et al . 15,16 investigated the fermentation of maltodextrin and glucose for efficient 2,3‐BD production, with the highest yield (0.49 g g −1 ) by the strain under a combined substrate feeding strategy and optimized oxygen supply. However, K. oxytoca KMS006 does not efficiently ferment cassava starch owing to the low expression of amylolytic enzymes.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Khunnonkwao

Jantama

et al. 2020

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

BACKGROUND 2,3‐butanediol (2,3‐BD) is one of the important platform chemicals used for biofuels and biochemicals. To make an economically feasible production of 2,3‐BD, an optimization process with a cheap substrate is required. The best conditions for enzymatic hydrolysis and fermentation were investigated to achieve high concentration and yield of 2,3‐BD from cassava starch while reducing the production cost regarding enzyme usage, energy input and medium preparation. RESULTS A 2.5 L bioreactor containing 1 L working volume of sterilized and gelatinized starch (130 g L−1) was placed at room temperature (25 °C). When the temperature of the starch solution reached 85 °C, α‐amylase (200 U g−1 starch) was added to the solution. The enzymatic reaction was allowed to proceed while the temperature of the solution was simultaneously dampened to 45 °C within 1 h. The reaction was further maintained at 45 °C for another 2 h. After hydrolysis, 64.8 g L−1 2,3‐BD was produced with a yield of 0.46 g g−1, productivity of 1.25 g L−1 h−1 and few by‐products by Klebsiella oxytoca KMS006. Further improvements of 2,3‐BD (92.5 g L−1) and yield (0.49 g g−1) were observed in fed‐batch mode under a constant feeding rate of 3.5 g starch h−1. CONCLUSION With our developed strategy, costs related to energy consumption and medium preparation were reduced by using heat generated by autoclave as well as by neglecting the use of glucoamylase for hydrolysis of cassava starch. The highest yield of 2,3‐BD (0.49 g g−1) was achieved, which is 98% of theoretical maximum. The process may be applied to the economical production of 2,3‐BD. © 2020 Society of Chemical Industry

show abstract

Strategies to reduce the culture medium costs for a high‐yield and high‐selectivity bio‐based 2,3‐butanediol production

Tinôco,

Seldin,

Coutinho

et al. 2024

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Bio‐based 2,3‐butanediol (2,3‐BDO) production on a large scale depends on critical factors, such as culture medium, oxygen supply, pH and biosafety. In this study, three strategies for reducing culture medium costs were investigated: carbon/nitrogen (C/N) ratio; low‐cost nitrogen sources (crude yeast extract, brewer's yeast extract, corn steep liquor, urea, sodium nitrate, ammonium chloride, ammonium sulfate and dibasic ammonium phosphate); and microbial pH autoregulation. Batch fermentations were performed in a microaerobic environment using wild‐type and safe Paenibacillus peoriae NRRL BD 62. The yield and selectivity of 2,3‐BDO were used as control variables. A ratio between 2,3‐BDO production and glucose consumption (YP/S) of almost 80% and an optical purity of 87% levo‐2,3‐BDO, with no acetoin accumulation, were achieved in an NH4Cl‐based medium at C/N = 8.5 g/g and without external pH control, considering an initial glucose of 10 g/L. Based on Free on Board prices, a 63% savings in culture medium costs was achieved by replacing commercial yeast extract at pH 5. Validation assays with higher initial glucose concentrations showed a YP/S of 0.40 g/g and an optical levo‐/meso‐2,3‐BDO ratio of 1:0.8, with negligible acetoin accumulation. Therefore, the NH4Cl‐based medium at C/N = 8.5 g/g and without pH control was considered economically promising for high‐yield and high‐selectivity bio‐based 2,3‐BDO production.

show abstract

Using a combined oxygen‐supply and substrate‐feeding strategy to improve 2,3‐butanediol production by metabolically engineered Klebsiella oxytoca KMS005

Cited by 5 publications

References 26 publications

Bioprocess Development for 2,3-Butanediol Production from Crude Glycerol and Conceptual Process Design for Aqueous Conversion into Methyl Ethyl Ketone

Bioprocess Development for 2,3-Butanediol Production from Crude Glycerol and Conceptual Process Design for Aqueous Conversion into Methyl Ethyl Ketone

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Strategies to reduce the culture medium costs for a high‐yield and high‐selectivity bio‐based 2,3‐butanediol production

Contact Info

Product

Resources

About