Using a liver cell culture from Epinephelus coioides as a model to evaluate the nonylphenol-induced oxidative stress

Derakhshesh, Negin; Movahedinia, Abdolali; Salamat, Negin; Hashemitabar, Mahmoud; Bayati, Vahid

doi:10.1016/j.marpolbul.2017.06.049

Cited by 9 publications

(8 citation statements)

References 51 publications

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“…Considering that most cell lines obtained from tropical fish species grow in temperature range from 20 to 32 °C (Lu, Lannan, Rohovec, & Fryer, ), in the present study, temperature variations in this range were tested. This temperature range has been reported in other species such as Ctenopharyngodon idella (Lu et al, ), Epinephelus awoara ( Lai et al, ) and Epinephelus coioides ( Derakhshesh, Movahedinia, Salamat, Hashemitabar, & Bayati, ), as the most appropriate temperature range for growth of the cells derived from various tissues. In the present study, due to the eurythermal nature of L. klunzingri and its ability to live in a temperature range of 20 to 32 °C under normal conditions (Sheibani, ), there was no significant difference in the growth and proliferation of cultured cells at different temperatures.…”

Section: Discussionsupporting

confidence: 72%

“…FBS contains a variety of factors, including salts, vitamins and hormones, all essential for the cell growth (Aghili & Zarkesh Isfahani, ). In fact, the addition of FBS leads to an increase in mitotic cell division due to the activation of cell growth factors [such as transformation growth factor alpha (TGF‐α), transformation growth factor beta (TGF‐β) and epithelial growth factor (EGF)] and cellular DNA synthesis (Derakhshesh et al, ). Poisa‐Beiro et al () cultured the brain and kidney cells of Sparus aurata in a medium containing 20% FBS at 27–30 °C.…”

Section: Discussionmentioning

confidence: 99%

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Using ovarian and brain cell culture from the Mullet, Liza klunzingeri, to assess the inhibitory effects of benzo[a]pyrene on aromatase activity

Sheibani

Salamat

Movahedinia

et al. 2020

J of Applied Toxicology

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We assessed the toxic effects of benzo[a]pyrene (BaP) on cell viability, aromatase (Aro) activity and steroid production using ovarian and brain cell cultures obtained from Mullet, Liza klunzingeri. The brain and ovary were minced and digested, and the cells were suspended in Leibovitz's L-15 medium supplemented with 15% and 20% fetal bovine serum. The cell suspensions were seeded on 25-cm 2 cell-culture flasks at 1 × 10 6 cells/mL and incubated at 25 C for 2 weeks. A BaP concentration of 10 −5 mol/L was accepted as the half-maximal inhibitory concentration. Ovarian and brain cells were exposed to different concentrations of BaP [0 (control), 10 −6 , 2 × 10 −6 , 3 × 10 −6 mol/L] and incubated at 30 C. At different sampling times (0, 12, 24 and 48 h) 40 ng/10 5 cells of 1,4,6-androstatriene-3,17-dione (ATD) was added to each well. Aro activity, 17β-estradiol (E2) and ATD production were determined. The sensitivity of the cultivated ovarian and brain cells to BaP increased dose dependently. BaP was a potent inhibitor of Aro activity at 2 × 10 −6 and 3 × 10 −6 mol/L, both in the cultivated brain and ovarian cells at different sampling times, with 10 −6 mol/L BaP found to be the least potent Aro inhibitor. E2 production decreased from cultivated ovarian and brain cells treated by different concentrations of BaP. In conclusion, BaP is able to change the activity of Aro and disrupt the biosynthesis of estrogens, and thus affects reproduction in fish. K E Y W O R D Saromatase, benzo (a) pyrene, brain cell culture, Liza klunzingeri, ovarian cell culture, steroid

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Using ovarian and brain cell culture from the Mullet, Liza klunzingeri, to assess the inhibitory effects of benzo[a]pyrene on aromatase activity

Sheibani

Salamat

Movahedinia

et al. 2020

J of Applied Toxicology

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“…The same study linked NP to apoptosis, mediated by activation of poly(ADP-ribose) polymerase (PARP) and caspase 3 [108]. Although not clearly outlined by our bioinformatics analysis, very recent literature has connected NP with liver toxicity in mammals, including humans, as its pro-inflammatory properties negatively affects liver cells [109]. Behavior.…”

Section: Nonyl Phenol and Nonyl Phenol Ethoxylates: Endocrine Disruptormentioning

confidence: 78%

Potential Health Risks Linked to Emerging Contaminants in Major Rivers and Treated Waters

Kessler

Dawley

Crow

et al. 2019

Water

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The presence of endocrine-disrupting chemicals (EDCs) in our local waterways is becoming an increasing threat to the surrounding population. These compounds and their degradation products (found in pesticides, herbicides, and plastic waste) are known to interfere with a range of biological functions from reproduction to differentiation. To better understand these effects, we used an in silico ontological pathway analysis to identify the genes affected by the most commonly detected EDCs in large river water supplies, which we grouped together based on four common functions: Organismal injuries, cell death, cancer, and behavior. In addition to EDCs, we included the opioid buprenorphine in our study, as this similar ecological threat has become increasingly detected in river water supplies. Through the identification of the pleiotropic biological effects associated with both the acute and chronic exposure to EDCs and opioids in local water supplies, our results highlight a serious health threat worthy of additional investigations with a potential emphasis on the effects linked to increased DNA damage.

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“…Consequently, placental cells exposed to HP displayed a lipid signature reflecting oxidative stress (Figure 1). Oxidative stress and significant lipid peroxidation were common responses of fish liver cells and spermatozoa exposed to NP [30,31]. As with the other plasticizers, the endocrine-disruptive action of NP in catfish occurred through ROS formation and oxidative damage [32].…”

Section: Albaladejo Et Al Unpublished Results) Macrophages Of the Fre...mentioning

confidence: 99%

Plastics and plastic additives as inducers of oxidative stress

Pérez-Albaladejo

Solé

Porte

2020

Current Opinion in Toxicology

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Using a liver cell culture from Epinephelus coioides as a model to evaluate the nonylphenol-induced oxidative stress

Cited by 9 publications

References 51 publications

Using ovarian and brain cell culture from the Mullet, Liza klunzingeri, to assess the inhibitory effects of benzo[a]pyrene on aromatase activity

Using ovarian and brain cell culture from the Mullet, Liza klunzingeri, to assess the inhibitory effects of benzo[a]pyrene on aromatase activity

Potential Health Risks Linked to Emerging Contaminants in Major Rivers and Treated Waters

Plastics and plastic additives as inducers of oxidative stress

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