Using a Population Genetics Approach for a Preliminary Investigation concerning Species Boundaries inHerbertia(Iridaceae)

Genetic and ecological niche modeling of Calydorea crocoides (Iridaceae): an endemic species of Subtropical Highland Grasslands

“…At the population level, AMOVA ( Table 4 ) showed that most of the genetic variation was found within populations, suggesting a genetic structure characteristic of outcrossing plants ( Nybom and Bartish, 2000 ; Nybom, 2004 ). Estimates of genetic diversity within populations presented here were lower than estimates for other species of Iridaceae found in South American grasslands ( Tacuatiá et al , 2012a ; Stiehl-Alves et al , 2017 ). Genetic diversity of plant species can be affected by many factors, such as amplitude range, duration of life cycle, mating system, and seed dispersion capacity ( Hamrick and Godt, 1996 ; Nybom and Bartish, 2000 ; Nybom, 2004 ).…”

“…These Iridaceae have some characteristics in common, such as seed dispersal by gravity (i.e., the seeds are dispersed near to the parental) and being probably long lived perennials. In addition, outcrossing was established for species of Iridaceae, and usually cross-pollinated species retain higher levels of genetic polymorphism ( Hannan and Orick, 2000 ; Wróblewska et al , 2003 ; Wang et al , 2009 ; Tacuatiá et al , 2012a ; Stiehl-Alves et al , 2017 ). In turn, species with a narrow geographic range, being adapted to a restricted niche, as well as endangered species, tend to maintain lower levels of genetic variation than species with broad geographic distribution ( Hamrick and Godt, 1996 ; Hannan and Orick, 2000 ; Nybom and Bartish, 2000 ; Nybom, 2004 ; Szczecinska et al , 2016 ), but exceptions may occur ( Lorenz-Lemke et al , 2006 , 2010 ; Sede et al , 2012 ; Turchetto et al , 2016 ).…”

“…The size of the fragments was estimated using Ladder 100 bp (Ludwig Biotec, Porto Alegre, RS). Similar to the approach employed by Stiehl-Alves et al (2016 , 2017 ) and by Ng and Tan (2015) , reproducibility of ISSR banding patterns was tested with three repeated amplifications of five samples per population. These did not show differences in the pattern of amplified DNA fragments.…”

“…The fast pace of technological advances in the genomic and postgenomic eras has been greatly important for fostering knowledge on plant biology, but, when such data are not available, classical genetic analyses are still needed as a first step towards the description of biodiversity, especially for wild species ( Pérez et al , 2015 ). Studies concerning the estimation of genetic diversity have been done for several species of Iridaceae ( Hannan and Orick, 2000 ; Wróblewska et al , 2003 ; Sik et al , 2008 ; Wang et al , 2009 ; Tacuatiá et al , 2012a , b ; Stiehl-Alves et al , 2016 , 2017 ). Many molecular markers can be used as tools for population genetics studies in plants.…”

A taxonomic synopsis of Cypella (Iridaceae) in Brazil

“…The list of Iridaceae reported in this project encompasses 160 species distributed among 18 genera . Subsequently, knowledge on systematics and taxonomy of Iridaceae was expanded through increasing collections, phylogenetic approaches (Chauveau et al 2011;Lovo et al 2012;Alves et al 2014;Inácio et al 2017;Stiehl-Alves et al 2017); multivariate statistical treatments (Pastori et al 2018); and various new species publications (Lovo & Mello-Silva 2011;Deble et al 2012c;2015a;b;Aita et al 2013;Chauveau et al 2014;Gil et al 2014;Oliveira et al 2016;Inácio et al 2018). Despite considerable advances, various genera still need to be broadly and thoroughly explored to assess their taxonomic delimitation and infrageneric classification (Chauveau et al 2012;Pastori et al 2018).…”