Using a zero-inflated model to assess gene flow risk and coexistence of Brassica napus L. and Brassica rapa L. on a field scale in Taiwan

Su, Yuan-Chih; Wang, Po‐Shung; Yang, Jhih‐Ling; Hong, Hong; Lin, Tzu-Kai; Tu, Yuan-Kai; Kuo, Bo‐Jein

doi:10.1186/s40529-020-00294-2

Cited by 3 publications

(5 citation statements)

References 33 publications

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“…In greenhouse conditions, artificial hand pollination with transgenic B. napus resulted in a 100% outcrossing rate. However, in field conditions, spontaneous hybridization has an outcrossing rate, ranging from 0.02 to 2.78% in field conditions [34,70]. Due to several environmental factors, the outcrossing rate is much lower compared to greenhouse conditions.…”

Section: Discussionmentioning

confidence: 99%

Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp.

Sohn

Thamilarasan

Subramanian

et al. 2022

IJMS

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Interspecific hybridization between transgenic crops and their wild relatives is a major concern for transgene dispersal in the environment. Under controlled conditions, artificial hand pollination experiments were performed in order to assess the hybridization potential and the fitness of interspecific hybrids between Brassica rapa and genetically modified (GM) Brassica napus. Initially, six subspecies of B. rapa were hybridized with GM B. napus through hand pollination. In the resulting F1 hybrids, the combination of B. rapa ssp. narinosa (♀) × GM B. napus (♂) had the highest crossability index (16.9 ± 2.6). However, the F1 selfing progenies of B. rapa ssp. rapa (♀) × GM B. napus were found to be more effective in producing viable future generations with the highest crossability index (1.6 ± 0.69) compared to other subspecies. Consequently, they were used for the generation of F2 and F3 progenies. The 18 different morphological characteristics among the parental cross-combinations and F1 hybrid progenies were measured and visualized through hierarchical clustering. Different generations were found to be grouped based on their different morphological characteristics. The chromosome numbers among the interspecific hybrids ranged from 2n = 29 to 2n = 40. Furthermore, the SSR markers revealed the presence of genomic portions in the hybrids in comparison with their parental lines. There is a high possibility of transgene flow between GM B. napus and B. rapa. The study concluded that the interspecific hybrids between B. napus and B. rapa can be viable and can actively hybridize up to F3 generations and more. This suggests that the GM B. napus can disperse the transgene into B. rapa, and that it can pass through for several generations by hand pollination in a greenhouse environment.

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Section: Discussionmentioning

confidence: 99%

Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp.

Sohn

Thamilarasan

Subramanian

et al. 2022

IJMS

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“…The dispersal models were constructed by combining different observation models and kernels in this study. The zero-inflated models, such as the ZIP mode and zero-inflated negative binomial model, were usually used to describe the zero-excess count data 27 , 30 . Bensadoun et al 27 reported that the cross validation result of the dispersal model with the ZIP observation model (R 2 = 0.582) was better than that of the Poisson observation model (R 2 = 0.511).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous fat-tailed kernels have been used in dispersal studies, such as Cauchy 40 , log-sech 30 , and 2Dt 41 . Paradis et al 42 reported that the Cauchy kernel predicted long-distance dispersal events more frequently than the exponential model.…”

Section: Discussionmentioning

confidence: 99%

“…Because of the high proportions of zero value observations, the present study assumed that the CP grain count followed the zero-inflated Poisson (ZIP) distribution to account for zero-excess condition 28 . The ZIP distribution was first proposed by Lambert 29 , and several studies had applied the ZIP distribution to deal with the CP data 27 , 30 . The ZIP distribution consists of a Dirac distribution in zero and a Poisson distribution.…”

Section: Methodsmentioning

confidence: 99%

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Incorporating the field border effect to reduce the predicted uncertainty of pollen dispersal model in Asia

Lee²,

Yiu

et al. 2021

Sci Rep

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The presence of the field border (FB), such as roadways or unplanted areas, between two fields is common in Asian farming system. This study evaluated the effect of the FB on the cross-pollination (CP) and predicted the CP rate in the field considering and not considering FB. Three experiments including 0, 6.75, and 7.5 m width of the FB respectively were conducted to investigate the effect of distance and the FB on the CP rate. The dispersal models combined kernel and observation model by calculating the parameter of observation model from the output of kernel. These models were employed to predict the CP rate at different distances. The Bayesian method was used to estimate parameters and provided a good prediction with uncertainty. The highest average CP rates in the field with and without FB were 74.29% and 36.12%, respectively. It was found that two dispersal models with the FB effect displayed a higher ability to predict average CP rates. The correlation coefficients between actual CP rates and CP rates predicted by the dispersal model combined zero-inflated Poisson observation model with compound exponential kernel and modified Cauchy kernel were 0.834 and 0.833, respectively. Furthermore, the predictive uncertainty was reducing using the dispersal models with the FB effect.

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“…Another study found that a transgene carried by the C chromosome is less likely to be transferred in a B. rapa background than a gene carried by the A chromosome, and the chance of transfer varies across the C genome [ 52 ]. Even though it is a fact that the A and C genomes share a large degree of similarity, the safe-spot idea has been questioned by several authors, and contradicting evidence suggests that transgene insertion position may not lead to greater biosafety in terms of gene flow [ 9 , 11 , 50 , 58 ]. Subsequently, another piece of statistical evidence with the biased and unbiased model using a mixed population of different C chromosome numbers is the relative fitness of B. rapa BC 1 and F 1 hybrids.…”

Section: Fate Of Transgenes In Interspecific Hybrid Plantsmentioning

confidence: 99%

Interspecific Hybridization of Transgenic Brassica napus and Brassica rapa—An Overview

Sohn

Thamilarasan

Subramanian

et al. 2022

Genes

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In nature, interspecific hybridization occurs frequently and can contribute to the production of new species or the introgression of beneficial adaptive features between species. It has great potential in agricultural systems to boost the process of targeted crop improvement. In the advent of genetically modified (GM) crops, it has a disadvantage that it involves the transgene escaping to unintended plants, which could result in non-specific weedy crops. Several crop species in the Brassica genus have close kinship: canola (Brassica napus) is an ancestral hybrid of B. rapa and B. oleracea and mustard species such as B. juncea, B. carinata, and B. nigra share common genomes. Hence, intraspecific hybridization among the Brassica species is most common, especially between B. napus and B. rapa. In general, interspecific hybrids cause numerous genetic and phenotypic changes in the parental lines. Consequently, their fitness and reproductive ability are also highly varied. In this review, we discuss the interspecific hybridization and reciprocal hybridization studies of B. napus and B. rapa and their potential in the controlled environment. Further, we address the fate of transgenes (herbicide resistance) and their ability to transfer to their progenies or generations. This could help us to understand the environmental influence of interspecific hybrids and how to effectively manage their transgene escape in the future.

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Using a zero-inflated model to assess gene flow risk and coexistence of Brassica napus L. and Brassica rapa L. on a field scale in Taiwan

Cited by 3 publications

References 33 publications

Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp.

Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp.

Incorporating the field border effect to reduce the predicted uncertainty of pollen dispersal model in Asia

Interspecific Hybridization of Transgenic Brassica napus and Brassica rapa—An Overview

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