Using an Arabidopsis oligonucleotide microarray to analyze the secretory pathway genes' Response to TuMV infection in Brassica rapa

Omics Approach to Identify Factors Involved inBrassicaDisease Resistance

“…Genome sequences included those of Chinese cabbage (B. rapa), cabbage and kale (B. oleracea) and rapeseed (B. napus) (Chalhoub et al, 2014;Liu et al, 2014;Parkin et al, 2014;Wang et al, 2011). The genomes of B. rapa (A) and B. oleracea (C) have suffered a whole genome triplication (WGT), containing each three sub-genomes Parkin et al, 2014;Wang et al, 2011), being this a characteristic of the Brassicaceae tribe (Li et al, 2014). While the genomes of B. oleracea and B. rapa are highly similar in terms of total gene clusters/sequences and the gene number in each cluster, there are also a large number of species-specific genes in the two species.…”

“…early transcriptomic studies took advantage of the high degree of synteny in gene sequences between A. thaliana and Brassica spp. This highly genetic similitude has allowed the use of the A. thaliana microarrays to examine transcriptional changes associated with the response of B. napus cultivars to S. sclerotiorum infection at different time points following pathogen infection (Liu et al, 2005;Yang et al, 2007;Zhao et al, 2007) or to analyze the transcriptomic response to TuMV (turnip mosaic virus) infection in B. rapa (Li et al, 2014). Zhao et al (2009) to monitor gene expression in B. napus in response to S. sclerotiorum insult.…”