Using an F2 Screen to Search for Resistance Alleles to Bacillus thuringiensis Toxin in European Corn Borer (Lepidoptera: Crambidae)

Andow, David A.; Alstad, D. N.; Pang, Yan; Bolin, P. C.; Hutchison, W. D.

doi:10.1093/jee/91.3.579

Cited by 83 publications

(41 citation statements)

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“…F 2 screens of 1,418 isofemale lines (5,672 alleles) from Þeld populations in the United States and France, no alleles for resistance to Bt corn that produces Cry1Ab were detected (Andow et al 1998(Andow et al , 2000Bourguet et al 2003). Although laboratory selection of O. nubilalis strains has yielded several strains with moderate levels of resistance (Huang et al 1997, Bolin et al 1999), we are not aware of any that are reported to complete development on Cry1Ab-producing corn (Huang et al 2002).…”

Section: Discussionmentioning

confidence: 98%

Inheritance of Resistance to the Cry1Ab Bacillus thuringiensis Toxin in Ostrinia nubilalis (Lepidoptera: Crambidae)

Alves¹,

Spencer²,

Tabashnik³

et al. 2006

Journal of Economic Entomology

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Section: Discussionmentioning

confidence: 98%

Inheritance of Resistance to the Cry1Ab Bacillus thuringiensis Toxin in Ostrinia nubilalis (Lepidoptera: Crambidae)

Alves¹,

Spencer²,

Tabashnik³

et al. 2006

Journal of Economic Entomology

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“…Bioassays have been used to estimate resistance allele frequencies, but their failure to distinguish between heterozygotes and susceptible homozygotes usually necessitates huge sample sizes or multigenerational experiments to detect rare resistant homozygotes (9)(10)(11). DNA-based detection of resistance alleles in heterozygotes could increase efficiency Ͼ1,000-fold compared with conventional bioassays (11), yet such screening requires knowledge of the molecular genetic basis of resistance to Bt toxins in field populations.…”

mentioning

confidence: 99%

Three cadherin alleles associated with resistance to Bacillus thuringiensis in pink bollworm

Morin

Biggs

Sisterson

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

397

388

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Evolution of resistance by pests is the main threat to long-term insect control by transgenic crops that produce Bacillus thuringiensis (Bt) toxins. Because inheritance of resistance to the Bt toxins in transgenic crops is typically recessive, DNA-based screening for resistance alleles in heterozygotes is potentially much more efficient than detection of resistant homozygotes with bioassays. Such screening, however, requires knowledge of the resistance alleles in field populations of pests that are associated with survival on Bt crops. Here we report that field populations of pink bollworm (Pectinophora gossypiella), a major cotton pest, harbored three mutant alleles of a cadherinencoding gene linked with resistance to Bt toxin Cry1Ac and survival on transgenic Bt cotton. Each of the three resistance alleles has a deletion expected to eliminate at least eight amino acids upstream of the putative toxin-binding region of the cadherin protein. Larvae with two resistance alleles in any combination were resistant, whereas those with one or none were susceptible to Cry1Ac. Together with previous evidence, the results reported here identify the cadherin gene as a leading target for DNA-based screening of resistance to Bt crops in lepidopteran pests.

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“…Gould et al (5) estimated that before widespread use of Bt cotton that produces toxin Cry1Ac, the frequency of a major recessive Bt resistance allele was 1.5 ϫ 10 Ϫ3 in tobacco budworm (Heliothis virescens). Andow et al (6,7) found no individuals of European corn borer (Ostrinia nubilalis) with alleles conferring resistance to Bt corn in 279 isofemale lines (1,116 haplotypes) from two populations.…”

mentioning

confidence: 99%

Frequency of resistance to Bacillus thuringiensis in field populations of pink bollworm

Tabashnik

Patin

Dennehy

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

237

248

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Strategies for delaying pest resistance to genetically modified crops that produce Bacillus thuringiensis (Bt) toxins are based primarily on theoretical models. One key assumption of such models is that genes conferring resistance are rare. Previous estimates for lepidopteran pests targeted by Bt crops seem to meet this assumption. We report here that the estimated frequency of a recessive allele conferring resistance to Bt toxin Cry1Ac was 0.16 (95% confidence interval ‫؍‬ 0.05-0.26) in strains of pink bollworm (Pectinophora gossypiella) derived from 10 Arizona cotton fields during 1997. Unexpectedly, the estimated resistance allele frequency did not increase from 1997 to 1999 and Bt cotton remained extremely effective against pink bollworm. These results demonstrate that the assumptions and predictions of resistance management models must be reexamined.

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Using an F2 Screen to Search for Resistance Alleles to Bacillus thuringiensis Toxin in European Corn Borer (Lepidoptera: Crambidae)

Cited by 83 publications

References 0 publications

Inheritance of Resistance to the Cry1Ab Bacillus thuringiensis Toxin in Ostrinia nubilalis (Lepidoptera: Crambidae)

Inheritance of Resistance to the Cry1Ab Bacillus thuringiensis Toxin in Ostrinia nubilalis (Lepidoptera: Crambidae)

Three cadherin alleles associated with resistance to Bacillus thuringiensis in pink bollworm

Frequency of resistance to Bacillus thuringiensis in field populations of pink bollworm

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