Using an Indicator Displacement Assay to Monitor Glucose Oxidase Activity in Blood Serum

Zhang, Tianzhi; Anslyn, Eric V.

doi:10.1021/ol070280o

Cited by 53 publications

(37 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[33] Confirmation of the mechanism based on the fluorescence quenching of CdTe QDs: It is well known that d-glucose can be oxidized quickly to gluconic acid and H 2 O 2 in the presence of GOx. [26][27][28] To illustrate the mechanism of the fluorescence quenching of the CdTe QDs-GOx nanosensor after the introduction of glucose, catalase, which is a scavenger of H 2 O 2 , was used in the reactive system. Figure 5 shows that the addition of catalase to the nanosensor solution before glucose sensing blocks the decrease in the fluorescence and does not produce any influence on the fluorescence of the nanosensor.…”

Section: Resultsmentioning

confidence: 99%

“…[25] At present, glucose oxidase (GOx) has been widely employed in the determination of glucose based on the enzyme-catalyzed oxidation mechanism using optical and electrical methods. [26][27][28] However, the direct sensing of glucose with high sensitivity accompanied by the considerably enhanced activity of GOx has not yet been realized. Quantum dots (QDs), owing to their good optical characteristics, high catalytic effects, and high electron-transfer efficiency, have been used as a preferable material in enzyme-based biological analyses and applications.…”

Section: Introductionmentioning

confidence: 99%

“…Statistical analysis gave us a value for the detection limit as low as 0.10 mm towards the concentration of glucose, thus the sensitivity of this method is a clear improvement on other GOx-based glucose detection methods. [26][27][28] Therefore, our new assembled CdTe QDs-GOx nanosensor can be applied to the ratiometric detection of glucose with high sensitivity and simplicity.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A New Route to the Considerable Enhancement of Glucose Oxidase (GOx) Activity: The Simple Assembly of a Complex from CdTe Quantum Dots and GOx, and Its Glucose Sensing

Cao¹,

Ye²,

Tong³

et al. 2008

Chemistry A European J

110

View full text Add to dashboard Cite

A new complex consisting of CdTe quantum dots (QDs) and glucose oxidase (GOx) has been facilely assembled to achieve considerably enhanced enzymatic activity and a wide active temperature range of GOx; these characteristics are attributed to the conformational changes of GOx during assembly. The obtained complex can be simultaneously used as a nanosensor for the detection of glucose with high sensitivity. A mechanism is put forward based on the fluorescence quenching of CdTe QDs, which is caused by the hydrogen peroxide (H2O2) that is produced from the GOx-catalyzed oxidation of glucose. When H2O2 gets to the surface of the CdTe QDs, the electron-transfer reaction happens immediately and H2O2 is reduced to O2, which lies in electron hole traps on CdTe QDs and can be used as a good acceptor, thus forming the nonfluorescent CdTe QDs anion. The produced O2 can further participate in the catalyzed reaction of GOx, forming a cyclic electron-transfer mechanism of glucose oxidation, which is favorable for the whole reaction system. The value of the Michaelis-Menton constant of GOx is estimated to be 0.45 mM L(-1), which shows the considerably enhanced enzymatic activity measured by far. In addition, the GOx enzyme conjugated on the CdTe QDs possesses better thermal stability at 20-80 degrees C and keeps the maximum activity in the wide range of 40-50 degrees C. Moreover, the simply assembled complex as a nanosensor can sensitively determine glucose in the wide concentration range from micro- to millimolar with the detection limit of 0.10 microM, which could be used for the direct detection of low levels of glucose in biological systems. Therefore, the established method could provide an approach for the assembly of CdTe QDs with other redox enzymes, to realize enhanced enzymatic activity, and to further the design of novel nanosensors applied in biological systems in the future.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

A New Route to the Considerable Enhancement of Glucose Oxidase (GOx) Activity: The Simple Assembly of a Complex from CdTe Quantum Dots and GOx, and Its Glucose Sensing

Cao¹,

Ye²,

Tong³

et al. 2008

Chemistry A European J

110

View full text Add to dashboard Cite

show abstract

“…For the glucose concentration sensors based on the pH-sensitive hydrogels, the simulation results show that the sensitivity may decrease with the increasing ionic strength. Probably, the reason is that the ionic strength may affect the association state of the pendent fixed charge groups and the corresponding hydration affinity in solutions [55]. Fig.…”

Section: Geometry Of Hydrogel Stripmentioning

confidence: 99%

Simulation analysis of effect of ionic strength on physiochemical and mechanical characteristics of glucose-sensitive hydrogels

Luo

2009

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

“…Manipulation of materials and processes on a nanometer scale is opening a world of creative possibilities. The benefits on improving smart methods for precisely monitoring the glucose level, with high sensitivity, high reliability, fast response, good selectivity, and low cost [27][28][29][30][31][32]. The most widely used methods for the determination of glucose are based on the monitoring of hydrogen peroxide stoichiometrically produced during the oxidation of glucose by oxygen in the presence of glucose oxidase.…”

Section: Introductionmentioning

confidence: 99%

A nanosensor for determination of glucose based on silver nanoparticles as fluorescence probes

et al. 2015

View full text Add to dashboard Cite

afforded by nanoscale technologies are expected to have substantial impacts on almost all industries and areas of society (e.g., medicine, plastics, energy, electronics, and aerospace) [1][2][3][4][5]. The recent advancement of bioanalytical techniques involving the development of highly sensitive or selective analytical methods based on nanoscale materials/molecules have progressed with remarkable success. In this regard, silver nanoparticles (AgNPs) have received significant attention in constructing analytical and bioanalytical sensors. This relevance arises from their unusual optical, electronic, and chemical properties [6][7][8]. They have a high surface area, very small size (<20 nm) and high dispersion ability, spectrally selective coating [9, 10], surface-enhanced Raman scattering [11,12], and antibacterial activity [13]. Moreover, AgNPs exhibit optical properties which could not be observed either in molecular or in bulk metallic form [14][15][16][17].Fluorescence spectroscopy as a sensitive, simple, diverse, and nondestructive method can provide real time, in situ, and dynamic analytical information [18]. However, utilizing nanoparticles in fluorescence analysis has provided significant improvement in this area [19][20][21][22][23]. It should be mentioned that the fluorescence of metal clusters and thin films is well established based on Mooradian's observation of photoluminescence from bulk copper and gold [24]. Also several reports are available related to the fluorescence of silver nanoclusters [25,26].Glucose, a simple sugar (monosaccharide), is an important carbohydrate in biology. Cells use it as a source of energy and a metabolic intermediate and its lack or excess can produce detrimental influence on cellular functions. The glucose level in blood is considered as a clinical indicator for diabetes. Therefore, the monitoring of glucose level in blood with faster and more accurate methods is in great demand and a great deal of effort has been focused Abstract A nanosensor was designed for the enzymatic determination of glucose based on the fluorescence enhancement of silver nanoparticles (AgNPs). This enhancement is the result of the glucose oxidase-catalyzed oxidation of glucose. Silver nanoparticles were prepared through a chemical reduction by using trisodium citrate as the reducing agent and were characterized with UV-visible spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy. The experimental results showed that the increase of the AgNP fluorescence was linearly proportional to the concentration of glucose within its concentration ranges of 2.50 × 10 −5 -7.50 × 10 −3 M and 7.50 × 10 −3 -7.50 × 10 −2 M with a detection limit of 5.53 × 10 −7 M under the optimized experimental conditions. To evaluate the applicability of the nanobiosensor, determination of glucose in real samples was performed according to the developed procedure.

show abstract

Using an Indicator Displacement Assay to Monitor Glucose Oxidase Activity in Blood Serum

Cited by 53 publications

References 20 publications

A New Route to the Considerable Enhancement of Glucose Oxidase (GOx) Activity: The Simple Assembly of a Complex from CdTe Quantum Dots and GOx, and Its Glucose Sensing

A New Route to the Considerable Enhancement of Glucose Oxidase (GOx) Activity: The Simple Assembly of a Complex from CdTe Quantum Dots and GOx, and Its Glucose Sensing

Simulation analysis of effect of ionic strength on physiochemical and mechanical characteristics of glucose-sensitive hydrogels

A nanosensor for determination of glucose based on silver nanoparticles as fluorescence probes

Contact Info

Product

Resources

About