Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics

Batelli, Giorgia; Oh, Dong Ha; D’Urzo, Matilde Paino; Orsini, Francesco; Dassanayake, Maheshi; Zhu, Jian; Bohnert, Hans J.; Bressan, Ray A.; Maggio, Aurelio

doi:10.1007/978-1-62703-580-4_2

Cited by 9 publications

(7 citation statements)

References 67 publications

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“…T. salsuginea is an Arabidopsis -related extremophile, and analysis of the difference in gene expression between T. salsuginea and A. thaliana may promote our understanding of how plants cope with abiotic stresses [22,23]. To achieve this goal, we compared the gene expression data of GPX genes of T. salsuginea to the corresponding data of A. thaliana in the AtGenExpress (Table S4) [31].…”

Section: Resultsmentioning

confidence: 99%

“…Thellungiella salsuginea ( Eutrema salsugineum ), a Brassicaceae species closely related to A. thaliana [22], can survive in environments with high soil salinity, low temperature, or water deficiency [23,24]. Compared to A. thaliana , T. salsuginea was more tolerant not only to high salinity but also to oxidative stress [25], and the level of lipid hydroperoxides in T. salsuginea leaves under salt stress was remarkably lower than that of A. thaliana [26], indicating that the enzymatic antioxidant systems in T. salsuginea behaves more efficiently.…”

Section: Introductionmentioning

confidence: 99%

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The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions

Gao

Chen

et al. 2014

IJMS

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Glutathione peroxidases (GPX) catalyze the reduction of H2O2 or organic hydroperoxides to water or corresponding alcohols using reduced glutathione, which plays an essential role in ROS (reactive oxygen species) homeostasis and stress signaling. Thellungiella salsuginea (Eutrema salsugineum), a relative of Arabidopsis thaliana, displays an extremely high level of tolerance to salt, drought, cold and oxidative stresses. The enzymatic antioxidant systems may contribute to the stress tolerance of T. salsuginea. In the present study, we aimed at understanding the roles of the antioxidant enzymes in T. salsuginea by focusing on the GPX family. We identified the eight GPX genes in T. salsuginea, and the structure of the N-terminal domains indicated their putative chloroplastic, mitochondrial and cytoplasmic location. The exon-intron organization of these genes exhibited a conserved pattern among plant GPX genes. Multiple environmental stresses and hormone response related cis-acting elements were predicted in the promoters of TsGPX genes. The gene and protein expression profiles of TsGPXs in response to high level of salinity and osmotic stresses, in leaves and roots of T. salsuginea were investigated using real-time RT-PCR and western blotting analysis. Our result showed that different members of the GPX gene family were coordinately regulated under specific environmental stress conditions, and supported the important roles of TsGPXs in salt and drought stress response in T. salsuginea.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions

Gao

Chen

et al. 2014

IJMS

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“…As far as current knowledge allows such generalization, plant genomes appear to include most or all functions that are necessary for a halophytic lifestyle, but only a small percentage of all plants are halophytes (Batelli et al, 2014). The requisite functional traits have become modified by diverse influences over approximately 500 million years of evolution on land, generating salt-tolerant species (halophytes) from salt-sensitive plants (mesophytes).…”

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confidence: 99%

A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance

et al. 2016

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“…In the current study, all Arabidopsis accessions performed as winter annuals, which germinate in autumn, overwinter and then bolt and flower the following spring. Compared to A. thaliana , a longer vernalization period is required for flowering in Eutrema (Batelli et al, 2014). Accordingly, the Eutrema accessions in the current study also performed as winter annuals.…”

Section: Resultsmentioning

confidence: 99%

Different metabolic adaptation strategies after overwintering inEutremasp. andArabidopsisaccessions under field conditions in Germany

Zuther,

Erban,

Kopka

et al. 2024

Physiologia Plantarum

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Successful overwintering is a prerequisite for high fitness in temperate perennials and winter annuals and is highly dependent on increased freezing tolerance and timely balancing of deacclimation with growth resumption in spring. To assess fitness costs associated with overwintering and elucidate metabolic mechanisms underlying winter survival and the switch from acclimated freezing tolerance to growth resumption, we performed a comparative field study using 14 Eutrema salsugineum accessions, E. halophilum, E. botschantzevii and 11 Arabidopsis thaliana accessions differing in freezing tolerance. Winter survival and reproductive fitness parameters were recorded and correlated with phenological stage and metabolite status during growth resumption in spring. The results revealed considerable intraspecific variation in winter survival, but survival rates of the extremophyte Eutrema were not inherently better. In both Eutrema and A. thaliana, improved winter survival was associated with reduced reproductive fitness. Metabolic analysis by GC–MS revealed intrinsic differences in the primary metabolism of the two genera during deacclimation. Eutrema contained higher levels of several amino and chlorogenic acids, while Arabidopsis had higher levels of several sugars and sugar conjugates. In both genera, increased levels of several soluble sugars were associated with increased winter survival, whereas myo‐inositol has different roles in overwintering of Eutrema and A. thaliana. In addition, differences in amino acid metabolism and polyhydroxy acids levels after winter survival were found. The results provide strong evidence for a trade‐off between increased winter survival and reproductive fitness in both Eutrema and Arabidopsis and document inherent differences in their metabolic strategies to survive winter.

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Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics

Cited by 9 publications

References 67 publications

The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions

The Glutathione Peroxidase Gene Family in Thellungiella salsuginea: Genome-Wide Identification, Classification, and Gene and Protein Expression Analysis under Stress Conditions

A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance

Different metabolic adaptation strategies after overwintering inEutremasp. andArabidopsisaccessions under field conditions in Germany

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