Using bacteria to express and display anti-parasite molecules in mosquitoes: current and future strategies

Riehle, Michael A.; Jacobs-­Lorena, Marcelo

doi:10.1016/j.ibmb.2005.02.008

Cited by 97 publications

(90 citation statements)

References 46 publications

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“…cme.msu.edu/) revealed the closest match as a recently described bacterium with proposed genus and species names, T. anophelis CCUG 49520 T (Kampfer et al, 2006). Since no similar T. anophelis DGGE bands were evident in the water and larval midgut samples, the results of PCR-DGGE would appear to support the prevailing view that adult mosquito midguts are populated mostly by bacteria obtained opportunistically (Riehle and Jacobs-Lorena, 2005). We sought to confirm this by assaying specifically for T. anophelis 16S rRNA genes in the various environments using T. anophelis-specific PCR primers (Tha226f and Tha1293r), which were designed in this study (see Materials and methods).…”

Section: Resultsmentioning

confidence: 57%

“…Paratransgenics refers to the genetic transformation of a bacterium, typically a vector endosymbiont, to produce antiparasitic molecules, thus inhibiting parasite development (Conte, 1997;Beard et al, 2002). Since Anopheles mosquitoes are currently considered a dead end host for midgut bacteria that are believed to 'perish when the adult mosquito dies' (Riehle and Jacobs-Lorena, 2005), the current paradigm for paratransgenesis in Anopheles focuses on bacteria that opportunistically infect adult mosquitoes Favia et al, 2007;Riehle et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Thorsellia anophelis is the dominant bacterium in a Kenyan population of adult Anopheles gambiae mosquitoes

et al. 2007

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Anopheles gambiae mosquitoes are not known to harbor endosymbiotic bacteria. Here we show, using nucleic acid-based methods, that 16S rRNA gene sequences specific to a recently described mosquito midgut bacterium, Thorsellia anophelis, is predominant in the midgut of adult An. gambiae s.l. mosquitoes captured in residences in central Kenya, and also occurs in the aquatic rice paddy environment nearby. PCR consistently detected T. anophelis in the surface microlayer of rice paddies, which is also consistent with the surface-feeding behavior of A. gambiae s.l. larvae. Phylogenetic analysis of cloned environmental 16S rRNA genes identified four major Thorsellia lineages, which are closely affiliated to an insect endosymbiont of the genus Arsenophonus. Physiological characterizations support the hypothesis that T. anophelis is well adapted to the female anopheline midgut by utilizing blood and tolerating the alkaline conditions in this environment. The results suggest that aquatically derived bacteria such as T. anophelis can persist through mosquito metamorphosis and become well-established in the adult mosquito midgut.

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Section: Resultsmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Thorsellia anophelis is the dominant bacterium in a Kenyan population of adult Anopheles gambiae mosquitoes

et al. 2007

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“…Furthermore, the use of genetically modified bacteria to deliver antiparasite molecules has several advantages over the use of genetically modified vectors (14). Very little is currently known about mosquito-associated microbiota (14)(15)(16). Here, we show that bacteria of the genus Asaia are stably associated with An.…”

mentioning

confidence: 81%

“…Microorganisms associated with vectors could exert a direct pathogenic effect on the host (9), interfere with its reproduction (10,11), or reduce vector competence (12,13). Furthermore, the use of genetically modified bacteria to deliver antiparasite molecules has several advantages over the use of genetically modified vectors (14). Very little is currently known about mosquito-associated microbiota (14)(15)(16).…”

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confidence: 99%

Bacteria of the genus Asaia stably associate with Anopheles stephensi , an Asian malarial mosquito vector

Favia

Ricci

Damiani

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

412

571

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Here, we show that an ␣-proteobacterium of the genus Asaia is stably associated with larvae and adults of Anopheles stephensi, an important mosquito vector of Plasmodium vivax, a main malaria agent in Asia. Asaia bacteria dominate mosquito-associated microbiota, as shown by 16S rRNA gene abundance, quantitative PCR, transmission electron microscopy and in situ-hybridization of 16S rRNA genes. In adult mosquitoes, Asaia sp. is present in high population density in the female gut and in the male reproductive tract. Asaia sp. from An. stephensi has been cultured in cell-free media and then transformed with foreign DNA. A green fluorescent protein-tagged Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission. The larval gut and the male reproductive system were also colonized by the transformed Asaia sp. strain. As an efficient inducible colonizer of mosquitoes that transmit Plasmodium sp., Asaia sp. may be a candidate for malaria control. malaria ͉ symbiotic control ͉ insect vector

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“…Microorganisms associated with the insect may have an important role for human infectious diseases epidemiology. Once a key role bacterium is discovered, it may be modified in order to affect the pathogen development and, consequently, the disease transmission (Azambuja et al 2005, Riehle & Jacobs-Lorena 2005.…”

mentioning

confidence: 99%

First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association

Gusmão

Santos

Marini

et al. 2007

Mem. Inst. Oswaldo Cruz

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We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fedAedes aegypti (Diptera: Culicidae, Aedini) is the main urban vector for the human diseases yellow fever and dengue fever (Nasci & Miller 1996). The vector control, mainly by insecticide application and elimination of oviposition sites, has been used as the best solution to decrease the diseases incidence. Explore new strategies for blocking the insect transmitted diseases such as dengue fever is urgent in Tropical countries (Sperança & Capurro 2007). Microorganisms associated with the insect may have an important role for human infectious diseases epidemiology. Once a key role bacterium is discovered, it may be modified in order to affect the pathogen development and, consequently, the disease transmission (Azambuja et al. 2005, Riehle & Jacobs-Lorena 2005.The alimentary canal of the mosquito is composed by the foregut, midgut and hindgut. The foregut is involved primarily with ingestion, conduction and storage of food (Romoser 1996). In the alimentary canal of the mosquito three diverticula arise near the posterior end of the esophagus: two from the dorso-lateral and one from the ventral wall of the gut, all surrounded by a thin impermeable cuticle. The ventral diverticulum (VD), or crop, is large and may extend into the abdomen (Dapples & Lea 1974). Usually all three diverticula are filled with air bubbles and are used as food reservoirs (Thompson 1905, Consoli & Lourenço-de-Oliveira 1994. The sugar meal, such as floral nectar, is stored in the diverticula and passes slowly to the midgut, where it is digested (Thompson 1905).Microorganisms play important and often essential roles in the growth and development of many insect species. Despite the importance of these microbial associations with insects there are relatively few studies that elucidate their components and their roles in the interactions. Insects that rely on nutritionally poor diets tend to possess bacterial endosymbionts. Aphids, for example, which subsist solely on plant sap, harbor Buchnera spp., believed to provide amino acids and vitamins to their hosts (Douglas 1989). Blood is known to be severely deficient in essential B vitamins and some amino acids. Bloodsucking arthropods such as ticks, lice, bedbugs, reduviid bugs, and tsetse flies usually harbor symbiotic microorganisms (Buchner 1965). Symbiotic relationships have already been reported for blood feeding insects and some are very well established, as in the case of Rhodnius prolixus that maintains an association with the actinomycete bacteria Rhodococcus rhodnii (Dasch et al. 1984); and tsetse flies genus Glossina, which harbor three distinct symbiotic microorganisms: Wigglesworthia glossinidia, Sodalis glossinidius (commensal), and the parasitic microbe Wolbachia pipientis (Aksoy et al. 1997, Chen et al. 1999.Many key questions about bacteria within the mosquito's midgut remain largely unanswered, and ob...

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Using bacteria to express and display anti-parasite molecules in mosquitoes: current and future strategies

Cited by 97 publications

References 46 publications

Thorsellia anophelis is the dominant bacterium in a Kenyan population of adult Anopheles gambiae mosquitoes

Thorsellia anophelis is the dominant bacterium in a Kenyan population of adult Anopheles gambiae mosquitoes

Bacteria of the genus Asaia stably associate with Anopheles stephensi , an Asian malarial mosquito vector

First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association

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