Using Bio-Functionalized Magnetic Nanoparticles and Dynamic Nuclear Magnetic Resonance to Characterize the Time-Dependent Spin-Spin Relaxation Time for Sensitive Bio-Detection

Abstract

In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe3O4 particles labeled as Fe3O4-antiCRP. It was found the time-dependent spin-spin relaxation time, T2, of protons decreases as time evolves. Additionally, the ΔT2 of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 μg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins.

Mossbauer research of Fe/Co nanotubes based on track membranes

“…Biomagnetism finds applications in cell separation, biosensing, and therapeutics. The ability to control physical properties of nanostructures by varying shape, size, and chemical composition [7][8][9] provides broad prospects for their use in biocatalysis [10], biodetection [11,12], bioseparation [13], drug delivery [10,14,15], genes [16,17], and study of microrheological processes [18] as well as contrast liquids [10,18].…”

Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges

“…Furthermore, not only small molecules but also larger targets such as bacteria or cells are labeled with MPs; the unbound MPs are removed, and the relaxation time is measured. Liao et al used dynamic magnetic resonance to detect C-reactive protein (CRP), a key indicator of infectious/noninfectious diseases or an acute tissue . Dextran-coated magnetite nanoparticles were conjugated with specific anti-CRP antibodies.…”

“…Liao et al used dynamic magnetic resonance to detect C-reactive protein (CRP), a key indicator of infectious/noninfectious diseases or an acute tissue. 454 Dextran-coated magnetite nanoparticles were conjugated with specific anti-CRP antibodies. The interaction of magnetic nanoparticles with CRP was monitored as a decreasing change of the T 2 relaxation time, which corresponds to the concentration of the analyte in the sample.…”

Degradation mechanism and way of surface protection of nickel nanostructures

“…When PMMA is used, amide and carboxyl groups, which allow to attach proteins and drugs, are formed. This modification of the surface makes it possible to consider "NSs + coatings + cargo" in the context of such applications as biocatalysis [60], bio-detection [61] [62] [63], bioseparation [2], delivery of drugs and genes [64], etc.…”