Using biomarkers to address the impacts of pollution on limpets (Patella depressa) and their mechanisms to cope with stress

Silva, Cátia S. E.; Novais, Sara C.; Simões, Tiago; Márcia, Caramalho; Gravato, Carlos; Rodrigues, M. J.; Maranhão, Paulo; Lemos, Marco F.L.

doi:10.1016/j.ecolind.2017.09.046

Cited by 23 publications

(13 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showed not only high numbers of intestinal enterococci and E. coli, but also the presence of P. aeruginosa in the water samples collected near the sewage treatment plant (STP) (Table 1, Figure 3). Previous studies reported high loads of E. coli and enterococci that exceeded the acceptable levels in the same coastal waters [24], and high numbers of bacteria were detected in limpets in this same spot [25]. In Europe, before coastline dischargement, sewage can receive secondary (organic matter removal) or tertiary (nutrients and bacteria removal) treatment.…”

Section: Discussionmentioning

confidence: 87%

“…This can still be explained by the discharged waters from the STP and their probably poorly disinfection. Corroborating this, Silva et al [25] performed microbiological analysis of limpet samples near the same STP site and other surrounding areas. Their findings suggest that limpets near the STP discharges have higher levels of E. coli compared with the other two sites in the geographical area, with lower levels of the same bacteria.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

et al. 2019

Self Cite

View full text Add to dashboard Cite

To estimate the quality of coastal waters, European Union Directive 2006/7/EC provides guidelines to assess levels of faecal bacteria, including Escherichia coli and intestinal enterococci. These microbiological criteria are based on studies that determine the risk of bathers having diseases caused by enteric bacteria, not necessarily measuring the potential danger associated with the presence of nonenteric pathogens. The association between the presence of faecal contaminant indicators and nonenteric pathogenic microorganisms has not been well defined yet. The purpose of this study is to establish a relationship between Pseudomonas aeruginosa and microbiological indicators of faecal contamination. Presence of microbiological contamination in the coastal waters near the sewage treatment plant (STP) of Peniche (Portugal) was confirmed (P. aeruginosa 135.8 Colony Forming Unit/100 mL, Escherichia coli 1100.1 Most Probable Number/100 mL, intestinal enterococci 2685.9 MPN/100 mL) with much lower levels in the areas located south of the STP, along the main water coastal current (beach 1: 0.7 CFU/100 mL, 16.5 MPN/100 mL, 100.5 MPN/100 mL; beach 2: 0.3 CFU/100 mL, 74.0 MPN/100 mL, 145.9 MPN/100 mL, respectively). Analysis of Pearson's correlation revealed a strong positive correlation between E. coli and P. aeruginosa, suggesting E. coli as an indicator of P. aeruginosa presence.Pathogens 2020, 9, 13 2 of 10 throat diseases [11]. These indicators were based on conclusions from epidemiological studies that implicate the enteric bacteria E. coli and enterococci in cases of gastroenteritis associated with swimming. However, the majority of recently reported cases of infectious diseases linked with coastal bathing waters are of nonenteric origin [12], creating a requirement for nonenteric indicator organisms that must be additionally evaluated. Opportunistic bacteria, such as Pseudomonas aeruginosa [13], might better serve to indicate the occurrence of some of the nonfaecal pathogens [12], especially in bathing waters [14]. Taking into account the results found in a study from Mariño and coworkers, where a positive correlation was found between skin infections and the presence of P. aeruginosa in seawater [14], this opportunistic bacteria [13] might better serve to indicate the occurrence of some nonfaecal pathogens in bathing waters [12].Being rod-shaped, gram-negative, facultative aerobic bacterium, P. aeruginosa has minimal survival requirements [15,16] and a remarkable adaptation ability towards a variety of environmental conditions, being able to thrive in soil, water, animal hosts, hospital settings, soap, and even distilled water [17][18][19][20]. P. aeruginosa, as the origin of an extensive variety of infections, is a main cause of illness in immunocompromised individuals [21]. Some examples are endocarditis, urinary tract infections, pneumonia, gastrointestinal infections, and meningitis, and it is also a leading cause of septicaemia [19]. P. aeruginosa is the pathogen mostly implicated in folliculitis and ear infec...

show abstract

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Field and biomonitoring studies, concerning environmental pollution, rely on IBR for result support [48][49][50][51][52]. Fewer laboratory studies testify IBR's applicability within the scope of treatment comparison [47,53].…”

Section: Propranololmentioning

confidence: 99%

Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants

Pires¹,

Novais²,

Lemos³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Worldwide anthropogenic activities result in the production and release of poten-tially damaging toxic pollutants into ecosystems, thereby jeopardizing their health and continuity. Research studies and biomonitoring programs attend to this emerging problematic by applying and developing statistically relevant indexes that integrate complex biomarker response data to provide an holistic approach reflecting toxically induced alterations at the organism’s or population level. Ultimately, indexes allow simple result communication, enhancing policy makers understanding, so contributing for better resource and environmental managing policies. In this study three indexes , the Integrated Biomarker Response index (IBR), the Bioeffects Assessment Index (BAI) and the Principal Components Analysis (PCA), were evaluated for their sensitivity in revealing toxically induced stress patterns in cells of the diatom Phaeodactylum tricornutum under contaminant exposure. The set of biomarkers selected for index construction comprise the anti-oxidant enzymes APX, CAT and SOD, and the lipid peroxidation marker TBARS. Several significant inverse correlations with the concentration gradients applied, , was noticed for all indexes, though, the IBR excels for its reliability in delivering statistically significant dose-response patterns for four out of the five compounds tested.

show abstract

“…The obtained results demonstrated 13 and 10 differentiating metabolites in G. umbilicalis and P. elegans, respectively, indicating A. armata exudate altered fatty acid biosynthesis and metabolism in the organisms, although through a different way. Many studies have shown that pollution can change the composition of FAs from organisms in the aquatic environment [20,45,46,47].…”

Section: Discussionmentioning

confidence: 99%

“…Fatty acid profile (FAP) has also been used as a biochemical response to pollutant exposure [18,19,20]. Fatty acids (FAs) are amongst the main constituents of the cell membrane and are involved in a wide range of biological pathways, from the production and permeability of cell membrane to lipids main components, while also being signalling mediators and used as fuel in all metabolic systems [21].…”

Section: Introductionmentioning

confidence: 99%

<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Silva¹,

Simões²,

Félix³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

The red seaweed Asparagopsis armata exhibits a strong invasive behaviour and is included in the list of the “Worst invasive alien species threatening biodiversity in Europe”. This seaweed has been shown to produce a large diversity of halogenated compounds with effective biological effects, deeply affecting rockpool species. Therefore, the present study aimed to investigate the biochemical responses to sublethal concentrations of Asparagopsis armata exudate on two coastal organisms, the marine snail Gibbula umbilicalis and the rockpool shrimp Palaemon elegans. Antioxidant defences superoxide dismutase (SOD) and glutathione-S-transferase (GST), oxidative damage endpoints lipid peroxidation (LPO) and DNA damage, the neuronal parameter acetylcholinesterase (AChE), as well as the fatty acid profile were evaluated. Results revealed different metabolic responses between species, indicating that A. armata exudate affected the organisms through different pathways. Despite previous studies indicating that the exudate effected G. umbilicalis’ survival and behaviour, this does not seem to result from oxidative stress or addressed neurotoxicity. On the other hand, for P. elegans, an inhibition of AChE and the decrease of antioxidant capacity concomitant with the increase of LPO, suggests neurotoxicity and oxidative stress as mechanisms of exudate toxicity for this species. For fatty acids, there were different profile changes between species, also more pronounced for P. elegans with a general increase in PUFA with exudate exposure, which commonly means a defence mechanism protecting from membrane disruption. Nonetheless, the omega-3 PUFAs ARA and DPA were increased in both invertebrates, indicating a common mechanism regulation of inflammation and immunity responses to this stress. This work provides further insight on the mechanisms of invertebrate response and tolerance to an expanding coastal environmental stress as is the marine invader A. armata.

show abstract

Using biomarkers to address the impacts of pollution on limpets (Patella depressa) and their mechanisms to cope with stress

Cited by 23 publications

References 78 publications

Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants

<em>Asparagopsis armata</em> Exudate Cocktail: The Quest for the Mechanisms of Toxic Action of an Invasive Seaweed on Marine Invertebrates

Contact Info

Product

Resources

About