Using biomimetically mineralized collagen membranes with different surface stiffness to guide regeneration of bone defects

Abstract

Because guided bone regeneration (GBR) process is pronouncedly affected by the micro-environment in the defect, the surface stiffness of collagen membranes as a constituent part of the micro-environment was investigated in this study. The objective of this study was to manufacture biomimetically mineralized collagen membranes with controllable surface stiffness based on biomimetic strategy and to investigate the influences of surface stiffness on GBR process. The characterization and biocompatibility of membranes were examined in vitro. The mechanical properties of membranes were evaluated on macro and micro levels using tensile test and atomic force microscope, respectively. The critical-size cranial defect model and ectopic osteogenesis were chosen to employ their performances in vivo. The results indicated that the biomimetically mineralized collagen membranes with controllable surface stiffness were manufactured based on the biomimetic theory. The in vitro experiments showed that the mineralized collagen membrane with satisfactory surface stiffness can better promote the adhesion, proliferation, and osteogenic differentiation of mesenchymal stem cells. The membranes can perform excellently in both osteoinduction and osteoconduction, which results in effective manifestations in aspects of ectopic osteogenesis and GBR in vivo. Therefore, this biomimetically mineralized collagen membrane is a promising candidate for GBR treatment in future.

The Injectable Woven Bone-Like Hydrogel to Perform Alveolar Ridge Preservation With Adapted Remodeling Performance After Tooth Extraction

“…As shown in Figure 3 and Supplementary Figure S2, the product possessed of the typical characteristic of both intrafibrillar and extrafibrillar mineralization. The c-axis alignment of nHA was parallel to the longitudinal direction of the collagen fibril, which was quite similar to the mineralized collagen fibril constructed through bottom-up method and highly alike to that in the natural bone (Zhang et al, 2003;Zhai and Cui, 2006;Qi et al, 2018;Wang Y. et al, 2018). Meantime, the coarse fibrils could be obtained to highly imitate the woven bone.…”

Influence of molecular weight and concentration of carboxymethyl chitosan on biomimetic mineralization of collagen

“…With the increased demands of bone tissue engineering, biomimetic mineralized scaffold boasts its superiority for providing a macro-and micro-environment similar to that of natural bone, and thus extra seed cells or growth factors are non-required for its construction. The potential effects of guided bone regeneration with collagen membrane were proved to be inuenced by different mineralization degree, 22,43 with contributing factors like types of polymeric process-directing agents, MWs and concentrations, mineralization time and pattern. Our results support using CMC to surrogate NCPs in biomimetic mineralization and provide parameters of MWs and concentrations for manufacturing mineralized collagen scaffolds with different mineralization degree, and thus may provide valuable attributes for fabrication of preciselycontrolled "biomimetic" bone gra substitutes.…”

“…20 Recently, the introduction of carboxymethyl chitosan (CMC) as novel analogs of NCPs into biomimetic mineralization has drawn researchers' attention. [21][22][23] As an amphoteric polyelectrolyte derived from chitosan, CMC contains abundant carboxyl and amino groups; when the environmental pH deviates from its isoelectric point, CMC can transform into a cationic/anionic polyelectrolyte through protonation/ deprotonation reactions. 24,25 It can sequester calcium ions and inhibit the transformation of ACP into HAp crystals, with which it can surrogate NCPs function as selective inhibitors or promoters of biomimetic mineralization.…”

Supplemental periodontal regeneration by vertical ridge augmentation around dental implants. A preclinical in vivo experimental study

“…There are several possible explanations for this. First, the choice of membrane may have contributed (Rakhmatia et al, ; Wang et al, ). Specifically, a nonresorbable membrane may have been more effective in space maintaining, and a titanium‐reinforced membrane or titanium mesh may have improved space maintaining and bone regeneration.…”