Using Bulk Magnetic Susceptibility to Resolve Internal and External Signals in the NMR Spectra of Plant Tissues

Shachar‐Hill, Yair; Befroy, Douglas E.; Pfeffer, Philip E.; Ratcliffe, George

doi:10.1006/jmre.1997.1185

Cited by 12 publications

(9 citation statements)

References 26 publications

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“…Mycorrhizal roots and extraradical mycelia from the same plates were also harvested, extracted, and analyzed. Partitioning of 2 H across the divider was quantified by placing 1.2 mL of solution from the labeled side of the plate in the outer compartment of a dual-compartment NMR tube (Pfeffer et al, 1979) with 10 mm Gd(NO 3 ) 3 added as a shifting agent (Shachar-Hill et al, 1997). The same volume of solution from the unlabeled side was added to the inner compartment.…”

Section: H O Equilibration Measurementsmentioning

confidence: 99%

Carbon Uptake and the Metabolism and Transport of Lipids in an Arbuscular Mycorrhiza1

et al. 1999

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Section: H O Equilibration Measurementsmentioning

confidence: 99%

Carbon Uptake and the Metabolism and Transport of Lipids in an Arbuscular Mycorrhiza1

et al. 1999

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“…The relatively small area for shifted water is expected, since the extracellular volume (intercellular spaces, periplasmic spaces, etc.) is a small fraction of the total water volume of this tissue (see Shachar-Hill et al 8 …”

Section: Resultsmentioning

confidence: 99%

“…'Vacuum infiltration' is a gentle treatment routinely used in root NMR, 13 and is well known not to cause physiological or structural damage. 8 The segment, constrained by placement in a long 1.85 mm i.d. NMR tube filled with 10 mM GdDTPA 2À , was aligned parallel to the magnet B 0 direction.…”

Section: Methodsmentioning

confidence: 99%

“…Shachar-Hill and coworkers first exploited this fact 8 with immature primary maize roots and shoots, leeks and elongated algal cells (Chara), bathed in solutions of the paramagnetic agents GdDTPA 2À or DyDTPA-BMA. Each nuclear species, 1 H 2 O or 2 H 2 O, solutes containing 13 C (glucose, fructose), 14 N (nitrate, ammonium), 23 Na and 35 Cl ions, showed clean, well-resolved doublets with spectral splittings, in dimensionless ppm, independent of the nuclear species, 8 decisive proof that the splitting arises from BMS effects. [5][6][7] The evidence (a 15% decrease in unshifted intracellular water intensity 60-90 min after agent introduction, saturation transfer from the shifted bath water signal to the unshifted water resonance) also suggested that the paramagnetic species did permeate the apoplast (all the regions topologically outside the cell plasma membranes: cell walls, intercellular spaces, xylem channels, etc.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic susceptibility shift selected imaging (MESSI) and localized1H2O spectroscopy in living plant tissues

Shachar‐Hill

Picart

et al. 2000

NMR Biomed.

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“…High spatial resolution can be obtained enabling microscopic features to be distinguished (Veres et al, 1991 ;MacFall & Johnson, 1994 ;Bentrup, 1996 ;Shachar-Hill et al, 1997). MRI studies of, for example, root systems have been performed and have proved to be of great interest in growth studies (Brown et al, 1991 ;Southon & Jones, 1992 ;MacFall & Johnson, 1994).…”

Section: mentioning

confidence: 99%

Nuclear magnetic resonance imaging in studies of gravitropism in soil mixtures

Antonsen¹,

Johnsson²,

Futsæther³

et al. 1999

New Phytologist

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Gravitropic responses of oat coleoptiles were measured in different growth media ; humid air, natural soil and artificial soil (glass beads). The oat coleoptiles in soil and glass beads were monitored by NMR imaging, while those in humid air were imaged in darkness with an infrared-sensitive charge-coupled device (CCD) camera. The present study shows for the first time that gravitropic experiments can be performed in artificial soil using NMR imaging as a convenient and suitable recording method. Not only was it possible to follow the gravitropic curvatures in natural soil, but the artificial soil allowed plant images of sufficient spatial and temporal resolution to be recorded. The advantages of using artificial soil in magnetic resonance imaging studies are that the iron content of glass beads is very low compared with natural soil, and that the artificial soil matrix can easily be standardized with regard to particle size distribution and nutrient content. Two types of glass beads were used, the diameter of the small and the large beads being 300-400 and 420-840 µm, respectively. The growth rate of the coleoptiles in soil and in big beads was roughly the same and only slightly lower than in humid air, whereas small beads reduced the growth rate by approx. 16%. The bending rate of the coleoptiles during the gravitropic response was reduced by c. 65% in soil and 75% in bead mixtures relative to bending in air. It should be noted, however, that the maximum curvature of the coleoptile tip was of the same order in all cases, about 35m. This value may represent the largest possible curvature of the organ. The potential of NMR imaging to study how plant organs penetrate the soil under the influence of gravitropism, mechanical impedance and thigmotropism is also discussed.

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Using Bulk Magnetic Susceptibility to Resolve Internal and External Signals in the NMR Spectra of Plant Tissues

Cited by 12 publications

References 26 publications

Carbon Uptake and the Metabolism and Transport of Lipids in an Arbuscular Mycorrhiza1

Carbon Uptake and the Metabolism and Transport of Lipids in an Arbuscular Mycorrhiza1

Magnetic susceptibility shift selected imaging (MESSI) and localized1H2O spectroscopy in living plant tissues

Nuclear magnetic resonance imaging in studies of gravitropism in soil mixtures

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