2020
DOI: 10.3389/fenvs.2020.00011
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Using Cathodic Poised Potential Experiments to Investigate Extracellular Electron Transport in the Crustal Deep Biosphere of North Pond, Mid-Atlantic Ridge

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Cited by 3 publications
(6 citation statements)
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References 119 publications
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“…Enrichment cultures of electrotrophic communities are obtained on electrodes and show a specific enrichment of Archaea belonging to the Thermococcales and Archaeoglobales orders in microbial electrolysis cells (specially designed bio-electrochemical system) under hyperthermophilic conditions (80 °C) (Pillot et al 2018). In an environmental context other than hydrothermal sources, high-potential cathodic experiments were also conducted to enrich microorganisms in North Pond crustal samples capable of extracellular electron transport (EET) (Jones et al 2020). Subsequent analyses of these incubations, using meta-omic analysis, fluorescence in situ hybridization coupled to secondary ion mass spectrometry (FISH-SIMS), isotope labeling experiments, and chemical analysis were necessary to understand the microbial community and metabolic pathways.…”
Section: Perspectivesmentioning
confidence: 99%
“…Enrichment cultures of electrotrophic communities are obtained on electrodes and show a specific enrichment of Archaea belonging to the Thermococcales and Archaeoglobales orders in microbial electrolysis cells (specially designed bio-electrochemical system) under hyperthermophilic conditions (80 °C) (Pillot et al 2018). In an environmental context other than hydrothermal sources, high-potential cathodic experiments were also conducted to enrich microorganisms in North Pond crustal samples capable of extracellular electron transport (EET) (Jones et al 2020). Subsequent analyses of these incubations, using meta-omic analysis, fluorescence in situ hybridization coupled to secondary ion mass spectrometry (FISH-SIMS), isotope labeling experiments, and chemical analysis were necessary to understand the microbial community and metabolic pathways.…”
Section: Perspectivesmentioning
confidence: 99%
“…Effluents from FLOCS colonization chambers were continuously collected in OsmoSamplers (Jannasch et al ., 2004) to determine solute concentration changes over time and confirm fluid origin and reaction progression. All experiments were recovered in October 2017 and processed as detailed in the Appendix S1 following protocols of recent studies (Ramírez et al ., 2019; Jones et al ., 2020). After verifying DNA extraction protocols on test samples (Table S2, Figs S3 and S4), the microbial communities on the colonized materials were compared to each other, and also to prior community data from rock and fluid samples from this and other study sites, to determine the degree that inoculation origin, redox and nutrient conditions, mineral type, length of exposure or other factors contribute to biogeographic patterning.…”
Section: Introductionmentioning
confidence: 99%
“…Further, we also documented the presence of genes associated with iron reduction [mtrCAB (Pitts et al, 2003;Hartshorne et al, 2007;Edwards et al, 2020) within seven of the timepoints, which span 2 years (Figure 1)]. The mtrCAB genes are most closely related to the dissimilatory iron reducer Shewanella benthica (Supplementary File 2), which was also enriched in mineral colonizations from the North Pond aquifer (Jones et al, 2020). We also identified nine MAGs (Tully et al, 2018), encoding copies of genes linked to respiratory iron oxidation or reduction (Supplementary Table 2).…”
Section: Resultsmentioning
confidence: 90%
“…Tully et al (2018) also reconstructed metagenomeassembled genomes (MAGs) affiliated with the iron-oxidizing Zetaproteobacteria, which are known to adapt to fluctuating O 2 concentrations and advective flow regimes (Chiu et al, 2017;Blackwell et al, 2020), further solidifying the presence and significant contribution that iron-oxidizing bacteria make to the aquifer community. Recent mineral colonization and current generation on poised electrodes also demonstrated the presence of iron oxidizing bacteria and provided evidence for their ability to utilize insoluble electron acceptors (Jones et al, 2020).…”
Section: Resultsmentioning
confidence: 99%
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