Using CF0218-ELISA to distinguish Chlamydophila felis-infected cats from vaccinated and uninfected domestic cats

Ohya, Kenji; Okuda, Hideko; Maeda, Sadatoshi; Yamaguchi, Tsuyoshi; Fukushi, Hideto

doi:10.1016/j.vetmic.2010.05.026

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“…The current detection methods for C. felis include isolation and identification of the pathogen, enzyme-linked immunosorbent assay (ELISA), and PCR assay ( Clarkson and Philips 1997 ; Helps et al 2001 ; Ohya et al 2010 ; Wons et al 2017 ; Barimani et al 2019 ) However, these methods need sophisticated equipment for a PCR assay and a relatively lengthy readout time for ELISA. Thus, there is an urgent need for a simple and faster detection method.…”

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confidence: 99%

A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis

Liu,

Qian,

Wang

et al. 2023

Polish Journal of Microbiology

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Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis.

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confidence: 99%