Volunteers’ contribution to research is growing, especially since the beginning of the 21st century. Given the constant increase of Citizen Science initiatives, recruiting strategies have to be planned properly. Retention is pivotal as well, especially when time is invested in volunteers’ training. However, practically no follow-up data are available on retention after major Citizen Science initiatives. CSMON-LIFE (Citizen Science MONitoring) was a 42-month project (2014–2017) funded by the European Commission in the framework of the LIFE+ programme (LIFE13 ENV/IT/842). It aimed at increasing awareness on Citizen Science among citizens, researchers, and decision makers in Italy. During CSMON-LIFE, recruitment was based on extensive awareness raising actions on different media. In total, 5558 volunteers were engaged in different field activities during the project and its follow-up. They gathered a total of 30062 geo-referenced observations, each with an image of the reported organism. Their activities were organized in campaigns, each devoted to a different topic. This study aims at investigating volunteers’ performance and retention in the funded period of CSMON-LIFE (December 2014–November 2017) and in its after-LIFE follow-up period (December 2017–November 2020), for a total of 72 months.