Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

Mahelková, Gabriela; Jirsová, Kateřina; Stangova, Petra Seidler; Palos, Michalis; Vesela, Viera; Fales, Ivan; Jirásková, N; Dotrelová, D

doi:10.1111/cxo.12455

Cited by 25 publications

(13 citation statements)

References 39 publications

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“…Patient symptoms were assessed subjectively using a survey questionnaire symptom score. There was statistically significant improvement in the subjective symptom score in 6 of 10 studies, 7,8,10,13,14,16 and there was improvement that was not statistically significant in 2 studies. 9e11 One study reported no change in symptom scores, 15 and the final study did not report on patient symptoms.…”

Section: Severe Dry Eye Diseasementioning

confidence: 89%

Autologous Serum-Based Eye Drops for Treatment of Ocular Surface Disease

et al. 2020

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Purpose: To describe the safety and effectiveness of using autologous serum-based eye drops for the treatment of severe dry eye and persistent corneal epithelial defect.Methods: Literature searches of the PubMed and Cochrane Library databases were conducted most recently in March 2019. The searches identified 281 citations, which were reviewed in abstract form. Of these, 48 were selected for a full-text review, and 13 met the inclusion criteria and were assigned a quality-of-evidence rating by the panel methodologist. Eight of these studies were rated level II and 5 were rated level III; there were no level I studies.Results: This analysis included 10 studies of the use of autologous serum-based eye drops for severe dry eye disease and 4 studies of persistent epithelial defect. Several studies showed good effectiveness, with some improvement in symptoms, signs, or both. Eight of the studies reported improved symptoms for severe dry eye disease, and all noted improvement in at least 1 clinical sign. For persistent epithelial defects, all of the studies showed improvement, with 3 of the 4 demonstrating an improvement rate of more than 90%. Adverse events were rare.Conclusions: Although autologous serum-based tears may be effective in the treatment of severe dry eye and persistent epithelial defect, conclusions are limited owing to the absence of controlled trials.

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Section: Severe Dry Eye Diseasementioning

confidence: 89%

Autologous Serum-Based Eye Drops for Treatment of Ocular Surface Disease

et al. 2020

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“…The balance of uid in corneal stroma is regulated by multiple tight junctions of endothelial cells and the action of Na + /K + ATPase pump. In this study, we found that corneal thickness increase signi cantly in the control group.Considering that the internal barrier function of the cornea was destroyed to make the uid in matrix unbalance [18].The function of mitochondria was destroyed, ATP production was decreased in corneal endothelial cells and epithelial cells,the permeability of endothelial cells was increased,these pathological changes destroyed the tight junction and Na + /K + ATPase pump function and aggravated matrix edema [19].And these abnormal changes were related to the age of diabetic patients [20].The corneal thickness was signi cantly lower in the treatment group,indicating that the function of corneal internal and external barrier was slightly damaged by dry eye disease and hyperglycemia, and the discomfort was further alleviated and visual function were further improved.…”

Section: Discussionmentioning

confidence: 99%

Effects of Shumu Decoction on Pathological Changes of Cornea in Patients with Diabetic Dry Eye

han

Wei

Tuo

et al. 2020

Preprint

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BACKGROUND:To observe the pathological changes of cornea and to clarify the effect Shumu Decoction on the cornea in diabetic dry eye.METHODS:The study assessed 200 eyes of 100 patients with diabetic dry eye.The treatment group 50 patients(100 eyes) and the control group 50 patients(100 eyes). The control group was treated with sodium hyaluronate eye drops. On the basis of the control group, the treatment group was treated by oral administration of Shumu Decoction.All participants underwent the examination of laser confocal microscope to obtain the image of cornea which were used to evaluate the differences between the two groups before and after treatment.RESULTS:After treatment,The corneal epithelial cell density in the treatment group was significantly higher than that in the control group(P <0.05) .The cell area of the two groups increased compared with that before treatment, but the area in the control group was significantly larger than that in the treatment group (P<0.05).The number of Langerhans cells in the control group were significantly higher than that in the treatment group(P<0.05).The morphology of Langerhans cells was characterized by large area and pseudopodia in the control group.The density, length of central corneal nerve fibers in the two groups were reduced compared to before treatment, but the decrease of nerve fiber in the control group was more obvious than that in the treatment group(P<0.05), and the morphology of nerve fibers was obvious change in the control group(P<0.05).At 4 weeks' treatment,the corneal endothelial cells showed morphological abnormality, there was significant difference in the ratio of endothelial cell hexagon between the two groups, and the corneal thickness increased significantly in the control group(P<0.05).CONCLUSION:Shumu Decoction can delay the loss of corneal cells and nerve fibers and restore the normal function of cornea.Those changes may alleviate the symptoms of patients with diabetic dry eye

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“…Levy et al [64] also reported an increase in corneal sub-basal nerves and a decrease in dendritic cell density after 6 months of treatment with topical 0.05% cyclosporine in Sjögren's syndrome. IVCM also demonstrated decreased corneal basal epithelial cell density and reduced numbers of nerve beadings after treatment with autologous serum eye drops [65,66].…”

Section: In Vivo Confocal Microscopymentioning

confidence: 93%

Objective Imaging Diagnostics for Dry Eye Disease

Han

Liu

Mohamed-Noriega

et al. 2020

Journal of Ophthalmology

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Traditional diagnostic tests for dry eye disease (DED), such as fluorescein tear film break-up time and the Schirmer test, are often associated with poor reproducibility and reliability, which make the diagnosis, follow-up, and management of the disease challenging. Advances in ocular imaging technology enables objective and reproducible measurement of changes in the ocular surface, tear film, and optical quality associated with DED. In this review, the authors will discuss the application of various imaging techniques, such as, noninvasive tear break-up time, anterior segment optical coherence tomography, in vivo confocal microscopy, meibography, interferometry, aberrometry, thermometry, and tear film imager in DED. Many studies have shown these devices to correlate with clinical symptoms and signs of DED, suggesting the potential of these imaging modalities as alternative tests for diagnosis and monitoring of the condition.

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Using corneal confocal microscopy to track changes in the corneal layers of dry eye patients after autologous serum treatment

Cited by 25 publications

References 39 publications

Autologous Serum-Based Eye Drops for Treatment of Ocular Surface Disease

Autologous Serum-Based Eye Drops for Treatment of Ocular Surface Disease

Effects of Shumu Decoction on Pathological Changes of Cornea in Patients with Diabetic Dry Eye

Objective Imaging Diagnostics for Dry Eye Disease

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