Using CRISPR-Cas9-based genome engineering tools in Drosophila melanogaster

Trivedi, Deepti

doi:10.1016/bs.pmbts.2021.01.006

Cited by 2 publications

(2 citation statements)

References 110 publications

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“…TALENs has been applied in the silkworm BmBLOS2 gene, resulting in an oily silkworm phenotype and succeeding in B. mori clock gene period e knockout; gene knockout of Ostrinia furnacalis sex pheromone related receptor gene resulted in abnormal mating behavior [30,31]. The CRISPR/Cas system has also been used in D. melanogaster [9], A. aegypti [10], Locusta migratoria [32] and Blattella germanica [33]. However, these techniques have not been applied in M. alternatus.…”

Section: Discussionmentioning

confidence: 99%

“…After innovation of zinc finger nucleases (ZFNs), upon emergence of transcription activator-like effecter nuclease (TALENs) and CRISPR/Cas, targeted genome-editing technology is becoming more efficient, less costly and easier to operate. In addition to common model insects such as D. melanogaster, Culex and Tribolium castaneum, the most updated targeted genome-editing technology, the CRISPR/Cas system, is also widely used in Diptera [9,10], Hymenoptera [11], Hemiptera [12], Lepidoptera [13][14][15] and Orthoptera [16]. Therefore, it is urgent to develop a feasible gene-editing system for M. alternatus and expand the system's prevention methods.…”

Section: Introductionmentioning

confidence: 99%

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RNA Interference-Mediated Knockdown of Tryptophan 2,3-Dioxygenase and Kynurenine-3-Monooxygenase in Monochamus Alternatus: Implications for Insect Control

Zhang

Weng

et al. 2023

Forests

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Monochamus alternatus Hope (Coleoptera: Cerambycidae), an invasive beetle that has caused billions of dollars in economic losses, is a serious pest of Pinus massoniana in many Asian countries. Clarifying the eye pigment gene and its knockdown phenotype of M. alternatus can provide functional gene identification and a marker for screening of gene editing, as well as help develop new control ideas. In this study, we first screened the transcriptome and found one homologous gene of tryptophan 2,3-dioxygenase (TDO) and one of kynurenine-3-monooxygenase (KMO). By measuring the expression levels of TDO and KMO in different developmental periods, it was indicated that TDO and KMO were expressed in various stages of M. alternatus. The gene expression of MaKMO was higher than MaTDO, showing high expression after pupation and decreasing at the beginning of eclosion. MaTDO and MaKMO were knocked down using RNA interference technology in different periods of expression, and the temporal expression changes were obtained using RT-qPCR technology. The results showed that the expressions of MaTDO and MaKMO were significantly inhibited by the injection of dsRNA; the expressions of MaTDO at 48 h, 96 h and after pupation were 21.9%, 32.3% and 59.2%, respectively, meanwhile, those of KMO were 23.4%, 25.0% and 69.7%, respectively. There was a significant change in eye color, and the beetles were able to pupate normally without their activity being affected. Therefore, both MaTDO and MaKMO can be used as tag genes for M. alternatus. A dominant marker system based on eye color can be developed for the genetic manipulation and control of M. alternatus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNA Interference-Mediated Knockdown of Tryptophan 2,3-Dioxygenase and Kynurenine-3-Monooxygenase in Monochamus Alternatus: Implications for Insect Control

Zhang

Weng

et al. 2023

Forests

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Classification, biology and entomopathogenic fungi-based management and their mode of action against Drosophila species (Diptera: Drosophilidae): a review

Vivekanandhan,

Swathy,

Sarayut

et al. 2024

Front. Microbiol.

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This review provides a comprehensive analysis of the classification, biology, and management of Drosophila species (Diptera: Drosophilidae) with a focus on entomopathogenic fungi (EPF) as a biocontrol strategy. Drosophila species, particularly Drosophila suzukii, and Drosophila melanogaster have emerged as significant pests in various agricultural systems, causing extensive damage to fruit crops. Understanding their taxonomic classification and biological traits is crucial for developing effective management strategies. This review delves into the life cycle, behavior, and ecological interactions of Drosophila species, highlighting the challenges posed by their rapid reproduction and adaptability. The review further explores the potential of EPF as an eco-friendly alternative to chemical pesticides. The mode of action of EPF against Drosophila species is examined, including spore adhesion, germination, and penetration of the insect cuticle, leading to host death. Factors influencing the efficacy of EPF, such as environmental conditions, fungal virulence, and host specificity, are discussed in detail. By synthesizing current research, this review aims to provide valuable insights into the application of EPF and to identify future research directions for enhancing the effectiveness of EPF-based control measures against Drosophila species.

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Using CRISPR-Cas9-based genome engineering tools in Drosophila melanogaster

Cited by 2 publications

References 110 publications

RNA Interference-Mediated Knockdown of Tryptophan 2,3-Dioxygenase and Kynurenine-3-Monooxygenase in Monochamus Alternatus: Implications for Insect Control

RNA Interference-Mediated Knockdown of Tryptophan 2,3-Dioxygenase and Kynurenine-3-Monooxygenase in Monochamus Alternatus: Implications for Insect Control

Classification, biology and entomopathogenic fungi-based management and their mode of action against Drosophila species (Diptera: Drosophilidae): a review

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